TEM of Human Recombinant Alpha Synuclein Oligomers (Dopamine HCL Stabilized) (SPR-466)

HPLC of Alpha Synuclein Protein Oligomers (SPR-466) ran down a size exclusion column. Wavelength: 280 nm.

Evaluation of a-syn toxicity on primary mouse cortical neurons. Lactate dehydrogenase (LDH) is a soluble enzyme present in the cytosol that is released upon cell death. Toxicity was assessed with an LDH assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. *** p<0.001. Treatment with dopamine-stabilized oligomers was accompanied by a significant increase of LDH release.

Evaluation of a-syn toxicity on primary mouse cortical neurons. Mitochondrial dehydrogenase activity, which reduces yellow MTT to dark blue formazan crystals, is a reaction that is catalyzed in living cells. Cell viability was assessed with an MTT assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. ** p<0.01, *** p<0.001. Both types of PFFs led to a decrease of viability of approximately 25% independent of sonication. Dopamine-stabilized oligomers led to the strongest decrease of viability.