Annexin V Recombinant Protein, FITC, eBioscience BMS306FI-100 from Thermo Fisher Scientific

Product Specs
ItemAnnexin V Recombinant Protein, FITC, eBioscience
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberBMS306FI-100
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size100 Tests
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) ATM knock out alters calcium signalling and augments contraction in skeletal muscle cells differentiated from human urine-derived stem cellsCell Death DiscovApril 15, 2025Giulia Dematteis, Giulia Lecchi, Giulia Boni, Diana Pendin, Carla Distasi, Mariagrazia Grilli, Dmitry Lim, Luigia Grazia Fresu, Maria Talmon(PI) staining cells were incubated with Annexin V Recombinant Protein 1x (FITC; eBioscience™, Cat. # BMS306FI-100) and PI 1 mg/ml in Annexin V binding buffer (PBS 1x, Hepes 10 mM, NaCl 150 mm, CaCl2 2.5 mM) 15 min

(2) Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological MalignanciesMoleculesFebruary 16, 2023Paulina Biniecka, Saki Matsumoto, Axel Belotti, Jessie Joussot, Jian Fei Bai, Somi Reddy Majjigapu, Paul Thoueille, Dany Spaggiari, Vincent Desfontaine, Francesco Piacente, et al.death induced by NAMPT inhibitors, malignant cells were stained with ANNEXIN-V (ANXN, eBioscience, BMS306FI/300) and 7-aminoactinomycin D (7AAD, Immunotech, A07704) as described by the manufacturer and analyzed using

(3) Nicotinaldehyde, a Novel Precursor of NAD Biosynthesis, Abrogates the Anti-Cancer Activity of an NAD-Lowering Agent in LeukemiaCancers (Basel)January 27, 2023Saki Matsumoto, Paulina Biniecka, Axel Bellotti, Michel A. Duchosal, Aimable NahimanaAPO866 in the absence or presence of 10 μM of nicotinaldehyde and stained with annexin-V (eBioscience, BMS306FI/300, Life Technologies) and 7-aminoactinomycin D (7AAD, A07704, Beckman Coulter, Nyon, Switzerland) as described

(4) Highly Cytotoxic Molybdenocenes with Strong Metabolic Effects Inhibit Tumour Growth in MiceChemistry – A European JournalJanuary 18, 2023Valentin Fuchs, Klaudia Cseh, Michaela Hejl, Petra Vician, Benjamin Neuditschko, Samuel M. Meier‐Menches, Lukas Janker, Andrea Bileck, Natalie Gajic, Julia Kronberger, et al.discarded and the cell pellet resuspended with 1.5 μL FITC‐conjugated annexin V (Thermo Fisher, Catalog# BMS306FI‐300) in 150 mL binding buffer (10 mM HEPES/NaOH pH 7.4, 140 mM NaCl, 2.5 mM CaCl2) and incubated at 37 °C

(5) Circular RNA_HIPK3-Targeting miR-93-5p Regulates KLF9 Expression Level to Control Acute Kidney InjuryComputational and mathematical methods in medicineJanuary 1, 2023Zhengbiao Z, Liang C, Zhi Z, Youmin P.propidium iodide (PI) (P1304MP, Thermo Fisher Scientific, Waltham, MA, USA) and 5 μL of Annexin V/FITC (BMS306FI-100, Thermo Fisher Scientific) at room temperature for 15 min. The cell apoptosis was determined on a FACScan

(6) Chlamydia trachomatis inhibits apoptosis in infected cells by targeting the pro-apoptotic proteins Bax and BakCell death and differentiationOctober 1, 2022Waguia Kontchou C, Gentle IE, Weber A, Schoeniger A, Edlich F, Häcker G.were collected and wash in annexin binding-buffer, then incubated with Annexin V-FITC (Invitrogen, #BMS306FI-300) and Live Dead Fixable Far Red (Life Technologies, #L10120) for 30 min on ice. Prior to adding of 2%

(7) Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunityNature communicationsJune 27, 2022Wiernicki B, Maschalidi S, Pinney J, Adjemian S, Vanden Berghe T, Ravichandran KS, Vandenabeele P.Binding Buffer and stained with Annexin V-APC (#BMS306APC/100, eBioscience, 1:100) or Annexin-FITC (#BMS306FI, ThermoFisher, 1:100), 1.25 µM SytoxBlue® (#S11348, Molecular Probes,) and alternatively 1 µg/ml 7-AAD

(8) Gut microbiota severely hampers the efficacy of NAD-lowering therapy in leukemiaCell Death & DiseaseApril 8, 2022Oussama ElMokh et al.absence of NAD+ precursors. APO866-induced cell death was determined using ANNEXIN-V (ANN; eBioscience, BMS306FI/300) and 7 aminoactinomycin D (7AAD; Immunotech, A07704) stainings as described by the manufacturer and

(9) Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic LeukemiaCancer Immunology ResearchMarch 1, 2022Natalia Colomar-Carando, Laurent Gauthier, Pietro Merli, Fabrizio Loiacono, Paolo Canevali, Michela Falco, Federica Galaverna, Benjamin Rossi, Frédéric Bosco, Mélody Caratini, et al.was analyzed by assessing 7AAD (catalog no.: 51-68981E; BD Biosciences) and AnnV-FITC (catalog no.: BMS306FI-300; Thermo Fisher Scientific) by flow cytometry. Percentage of specific lysis was calculated following

(10) Oxidized Low-Density Lipoprotein Induces Macrophage Production of Prothrombotic MicroparticlesJournal of the American Heart AssociationAugust 4, 2020Marchini JF, Manica A, Crestani P, Dutzmann J, Folco EJ, Weber H, Libby P, Croce K.(1X Tris Buffered Saline with 2.5 mmol/L CaCl2), and 2.5 µL of annexin V-fluorescein isothiocyanate (BMS306FI, eBioscience, San Diego, CA) to enable quantification of phosphatidylserine-positive microparticles.

(11) TFAP2A Induced ITPKA Serves as an Oncogene and Interacts with DBN1 in Lung AdenocarcinomaInternational Journal of Biological SciencesJanuary 1, 2020Zhou Guoren, Fan Zhaohui, Zhu Wei, Wang Mei, Wu Yuan, Shi Lin, Xu Xiaoyue, Zhang Xiaomei, Shen Bo× 106 cells/ml, then cells were stained with 7-AAD (Invitrogen, A1310) and Annexin V (eBioscience, BMS306FI-100). After 20min incubation at room temperature, cells were analyzed by a BD FACSCanto™ II flow cytometer.

(12) Reactive oxygen/nitrogen species contribute substantially to the antileukemia effect of APO866, a NAD lowering agentOncotargetNovember 19, 2019Anne-Julie Cloux et al.potential. Cell death analysis APO866-induced cell death was determined using ANNEXIN-V (ANN; eBioscience, BMS306FI/300) and 7-aminoactinomycin D (7AAD; Immunotech, A07704) stainings as described by the manufacturer and

(13) TFAP2A Induced KRT16 as an Oncogene in Lung Adenocarcinoma via EMTInternational Journal of Biological SciencesJune 2, 2019Liu Yuanhua, Qian Pudong, Zhu Wei, Wu Yuan, Liu Delin, Zhang Yan, Liang Geyu, Shen Bo× 106 cells/ml, then cells were stained with 7-AAD (Invitrogen, A1310) and Annexin V (eBioscience, BMS306FI-100). After 20min incubation at room temperature, cells were analyzed by a BD FACSCanto™ II flow cytometer.

(14) Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesisJournal of Biomedical ScienceDecember 1, 2016Daria Belik, Hilda Tsang, John Wharton, Luke Howard, Carmelo Bernabeu, Beata Wojciak-StothardeBioscience, USA) was used to label endothelium- derived MPs. Human Annexin 5-FITC antibody (cat. no BMS306FI/20, eBioscience) was also used to label the phosphatidylserine-rich MPs. Scanning electron microscopy Human

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Annexin V Recombinant Protein, FITC, eBioscience from Thermo Fisher Scientific

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Annexin V Recombinant Protein, FITC, eBioscience from Thermo Fisher Scientific

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