Fig 1: NLRP3 overexpression rescues the effects of NAT10 knockdown on OV cell malignancy. a. RT-qPCR confirmed successful NLRP3 overexpression in two OV cell lines (P < 0.001). (n = 3, biological replicates). b, c. CCK-8 tests revealed that NLRP3 overexpression revived cell proliferation in OV cells with NAT10 silencing. (P < 0.001). (n = 3, biological replicates). d, e. Transwell assays indicated that NLRP3 overexpression enhanced cell migration in NAT10-silenced OV cells (P < 0.001). (n = 3, biological replicates). f, g. Transwell assays also showed that NLRP3 overexpression increased cell invasion in NAT10-silenced OV cells (P < 0.001). (n = 3, biological replicates). h, i. Western blot analysis (h) and quantitative analysis (i) of migratory and invasive markers (N-cadherin, Vimentin, MMP2, MMP9) in OV cells from different groups. GAPDH served as the internal loading control, and data are presented as mean ± SD (P < 0.001). (n = 3, biological replicates)