Fig 1: Effects of HGF on proteins involved in iron metabolism. (A) Hepcidin mRNA expression was raised by ethanol stimulation and decreased by HGF treatment; (B,C) alterations in Fpn1, DMT1 and FTH1 caused by Nfe2l2 knockdown after HGF pretreatment detected by western blotting under ethanol exposure. **, P<0.01; ns, P>0.05. DMT1, divalent metal-ion transporter 1; Fpn1, ferroportin; FTH1, ferritin heavy chain 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HGF, hepatocyte growth factor; PBS, phosphate buffered saline; scramble, randomly interrupted sequence; siRNA, small interfering RNA.
Fig 2: Effects of exogenous HGF on ferroptosis in cardiomyocytes. (A) The effect of HGF on cell viability (CCK8); (B) the effect of HGF on GPX4; (C) the effect of HGF on LIP; (D) the effect of HGF on cell survival by calcein staining; (E) MDA concentrations of different HGF doses; (F) GPX4 concentrations of different HGF doses; (G) LIP levels of different HGF doses; (H) MDA concentrations following Nfe2l2 knockdown in ethanol-treated cells; (I) GPX4 concentrations following Nfe2l2 knockdown in ethanol-treated cells; (J) LIP levels following Nfe2l2 knockdown in ethanol-treated cells; (K) Cell survivals by calcein staining following Nfe2l2 knockdown in ethanol-treated cells. **, P<0.01; ns, P>0.05. CCK8, Cell Counting Kit-8; GPX4, glutathione peroxidase 4; HGF, hepatocyte growth factor; LIP, labile iron pool; MDA, malondialdehyde; MFI, mean fluorescence intensity; PBS, phosphate buffered saline; scramble, randomly interrupted sequence; siRNA, small interfering RNA.
Fig 3: Levels of serum HGF and effects of ethanol on cardiomyocytes. (A) Violin map displaying HGF concentrations; (B) CCK8 test for cardiomyocyte viability in response to various strategies for alcohol concentration; (C) effects of various alcohol intervention doses on cell survival by calcein staining. **, P<0.01. ACM, alcoholic cardiomyopathy; CCK8, Cell Counting Kit-8; Ctrl, control; HGF, hepatocyte growth factor.
Fig 4: Investigating the key node of HGF against ferroptosis in cardiomyocytes. (A) The intersection of two datasets; (B) subnetwork analysis and critical target mining; (C) Nrf2 expression in the cytoplasm and nucleus following HGF treatment. HGF, hepatocyte growth factor.
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