Fig 1: KCTD7 is a component of the Cullin-3 E3 ligase complex and interacts with calpains.a Co-IP analysis of Flag-tagged KCTD7 and myc-tagged Cullin-3 in HEK293 cells. b Co-IP analysis of KCTD7 mutant constructs and Cullin-3-Myc in HEK293 cells. c Co-IP analysis of KCTD7 deletion constructs and Cullin-3-Myc in HEK293 cells. d Co-IP analyses of Flag-tagged KCTD7, myc-tagged Cullin-3, and HA-tagged Rbx1 in HEK293 cells. e Co-IP analyses of GFP-tagged KCTD7 and Flag-tagged calpain 1, calpain 2, and CAPNS1 in HEK293 cells. f Confocal microscopy of HeLa cells showing KCTD7-GFP co-distribute with calpain 1-Flag, calpain 2-Flag, and CAPNS1-Flag. Trace outline is used for line-scan (white dashed line) analysis of relative fluorescence intensity of indicated protein signals. Scale bar, 10 μm. g Co-IP analyses of myc-tagged Cullin-3 with Flag-tagged calpain 1, calpain 2 and CAPNS1 in HEK293 cells. h Co-IP analyses of Flag-tagged KCTD7 mutant constructs with endogenous calpain 1, calpain 2, and CAPNS1 in HEK293 cells.
Fig 2: Schematic model of calpain regulation by the Cullin-3–RBX1–KCTD7 complex.Shown is a comparison between WT conditions and deficiency of KCTD7 leading to calpain dysregulation and activation of a pathogenic cascade.
Supplier Page from R&D Systems, a Bio-Techne Brand for CUL3/RBX1 Complex His-tag (Neddylated) Protein
Available conjugates: Sizes Available: 25 ug