Alt-R S.p. dCas9 Protein V3 1081066 from Integrated DNA Technologies

Product Specs
ItemAlt-R S.p. dCas9 Protein V3
CompanyIntegrated DNA Technologies
Price Supplier Page View Company Product Page
Catalog Number1081066
Applicationsgenome editing, CRISPR
TypeEnzyme

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Integrated DNA Technologies

1710 Commercial Park

Coralville, Iowa 52241

United States

Phone: 800-328-2661

Company Profile

Website: http://www.idtdna.com

(1) Aberrant CDK4/6-driven cell-cycle reentry drives neuronal loss and defines a therapeutic target in C9orf72 ALS/FTDiScienceFebruary 20, 2026Ling Lian, Hayley Robinson, Noah Daniels, G. Aleph Prieto, Gunnar H.D. Poplawski, Rodrigo Lopez-Gonzalez,STEMCELL Technologies,Cat# 73954 Accutase,Gibco,Cat# A1110501 Cas9 recombinant protein,Integrated DNA Technologies,Cat# 1081066 Propidium Iodide,Invitrogen™,Cat# P43566 RNase A (DNase-free),Thermo Scientific,Cat# EN0531 "Poly(GR),

(2) A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactionsNucleic Acids ResJune 28, 2025Huijin Lee, Fahad Rashid, Jihee Hwang, James A London, Richard Fishel, James M Berger, Sua Myong, Taekjip Hatemperature (1°C/min). The dCas9–gRNA complex was assembled by mixing guide RNA and dCas9 protein (IDT, 1081066) at a ratio of 2:1 in Cas9 dilution buffer (30 mM 2-(4-(2-hydroxyethyl)-1-piperazinyl)-ethanesulfonic

(3) Transient EZH2 Suppression by Tazemetostat during In Vitro Expansion Maintains T-Cell Stemness and Improves Adoptive T-Cell TherapyCancer Immunology ResearchJanuary 9, 2025Yingqin Hou, Jaroslav Zak, Yujie Shi, Isaraphorn Pratumchai, Brandon Dinner, Wenjian Wang, Ke Qin, Evan W. Weber, John R. Teijaro, Peng Wuribonucleoprotein (RNP) complexes preparation, 0.6 uL Cas9 protein (IDT, Alt-R™ S.p. Cas9 Nuclease V3, Cat#1081066), 1 uL sgRNA (0.15 nmol in total of the mixture of three sgRNAs), and 3.4 uL Rnase free water were mixed

(4) Global loss of promoter–enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesisNature CancerOctober 30, 2024Yizhou Zhu, Hayan Lee, Shannon White, Annika K. Weimer, Emma Monte, Aaron Horning, Stephanie A. Nevins, Edward D. Esplin, Kristina Paul, Gat Krieger, et al.4D nucleofector X unit (Lonza) with or without preincubation with equal molar Cas9/dCas9 protein (IDT, 1081058 and 1081066). We followed the protocol provided by IDT for the Lonza nucleofector system. Nucleofection of the cells

(5) A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactionsbioRxivOctober 29, 2024Huijin Lee, Jihee Hwang, Fahad Rashid, James A. London, Richard Fishel, James M. Berger, Sua Myong, Taekjip Hatemperature (1 °C/min). The dCas9-gRNA complex was assembled by mixing guide-RNA and dCas9 protein (IDT, 1081066) at a ratio of 2:1 in Cas9 dilution buffer (30 mM HEPES, 150 mM KCl, pH 7.5). RNA sequences are available

(6) Understanding Electrophoresis and Electroosmosis in Nanopore Sensing with the Help of the Nanopore Electro-Osmotic TrapACS NanoAugust 6, 2024Chenyu Wen, Sonja Schmid, Cees DekkerPreparation dCas9 proteins were purchased from Integrated DNA Technologies, Inc. (Alt-R S.p. dCas9 Protein V3,1081066, USA). All of the RNA and DNA were purchased from Ella Biotech GmbH (Germany). The sequences of all

(7) A frugal CRISPR kit for equitable and accessible education in gene editing and synthetic biologyNature CommunicationsAugust 3, 2024Marvin Collins, Matthew B. Lau, William Ma, Aidan Shen, Brenda Wang, Sa Cai, Marie La Russa, Michael C. Jewett, Lei S. Qireaction, were added to the following target concentrations: 2.1 ng uL−1 dCas9 V3 protein (IDT, cat no. 1081066); 5 nM chromoprotein plasmid DNA; 2.5 nM sgRNA plasmid DNA. Water was added to bring the final reaction

(8) Selection of extended CRISPR RNAs with enhanced targeting and specificityCommunications BiologyJanuary 12, 2024Ashley Herring-Nicholas, Hillary Dimig, Miranda R. Roesing, Eric A. Josephs#CAS9PROT-250UG), eCas9 enzyme (Sigma Aldrich #ESPCAS9PRO-50UG), or dCas9 enzyme (IDT Alt-R® S.p. dCas9 Protein V3 #1081066) and sgRNA were combined in equimolar amounts in Phosphate buffered saline, pH 7.4 - PBS (ThermoFisher,

(9) Selection of Extended CRISPR RNAs with Enhanced Targeting and SpecificitybioRxivJanuary 15, 2023Ashley Herring-Nicholas, Hillary Dimig, Miranda Roesing, Eric A. Josephs#CAS9PROT-250UG), eCas9 enzyme (Sigma Aldrich #ESPCAS9PRO-50UG), or dCas9 enzyme (IDT Alt-R® S.p. dCas9 Protein V3 #1081066) and sgRNA were combined in equimolar amounts in Phosphate buffered saline, pH 7.4 - PBS (ThermoFisher,

(10) A genetically engineered, stem-cell-derived cellular vaccineCell reports. MedicineDecember 20, 2022Cooper A, Sidaway A, Chandrashekar A, Latta E, Chakraborty K, Yu J, McMahan K, Giffin V, Manickam C, Kroll K, et al.and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Cas9 protein,IDT,Cat# 1081066 VEG-F protein,Peprotech,Cat# 100-20 Leukocyte activation cocktail,BD Biosciences,Cat# 550583 Vitronectin,StemCell Technologies

(11) HP1 proteins compact DNA into mechanically and positionally stable phase separated domainseLifeMarch 4, 2021Madeline M Keenen, David Brown, Lucy D Brennan, Roman Renger, Harrison Khoo, Christopher R Carlson, Bo Huang, Stephan W Grill, Geeta J Narlikar, Sy Reddingduring experiments. End-labeling of DNA was accomplished using dCas9 molecules. Specifically, dCas9 (IDT 1081066), Alt-R CRISPR-Cas9 tracrRNA (IDT 1072532), and an Alt-R CRISPR-Cas9 crRNA targeting bacteriophage λ

(12) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. WalterS.p. Cas9 H840A Nickase V3, 100 μg",IDT,Cat# 1081064 "Alt-R S.p. dCas9 Protein V3, 100 μg",IDT,Cat# 1081066 Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Bac-to-Bac Baculovirus Expression System

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Alt-R S.p. dCas9 Protein V3 from Integrated DNA Technologies

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Integrated DNA Technologies

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Alt-R S.p. dCas9 Protein V3 from Integrated DNA Technologies

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