PepTivator® SARS-CoV-2 Prot_S 130-126-700 from Miltenyi Biotec

(1) Direct pharmacological AMPK activation inhibits mucosal SARS-CoV-2 infection by reducing lipid metabolism, restoring autophagy flux and the type I IFN responseJournal of VirologyJuly 22, 2025Andrea Cottignies-Calamarte, Flora Marteau, Feifan He, Sandrine Belouzard, Jean Dubuisson, Daniela Tudor, Benoit Viollet, Morgane BomselT cell epitope peptide pools from either Spike or Nucleocapsid of SARS-CoV-2, respectively (Miltenyi 130–126-700 and 130–126-698, respectively), were used as stimulation sources at a final concentration of 500 ng/mL

(2) Preserved efficacy of lyophilized SARS-CoV-2 mRNA vaccine incorporating novel ionizable lipids after one year at 25 °CNPJ VaccinesJuly 1, 2025Elena Mata, Esther Broset, Carlos Matute, Andrei Stoian, Susana Adame, Teresa Alejo, Alexandre López, Beatriz Andrés, Juan Heredero, Diego de Miguel, et al.50 µg/mL of a peptide pool covering the immunodominant sequence domains of the S protein (Miltenyi, 130-126-700). Phorbol-12-myristate-13-acetate (PMA) 50 ng/mL (Merck, 524400) and ionomycin 500 ng/mL (Merck, 407953)

(3) Optimization of hypo-alloimmunogenic multispecific CAR-T and SARS-CoV-2-specific T cells for off-the-shelf adoptive cell therapyMolecular therapy. Methods & clinical developmentJune 12, 2025Ng KP, Seng MS, Leung W.PepTivators (Miltenyi Biotech), the overlapping peptide pools of the SARS-CoV-2 spike protein (S; cat. 130-126-700), membrane glycoprotein (M; cat. 130-126-702), and nucleocapsid phosphoprotein (N; cat. 130-126-698).

(4) Protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker and Th1 cytokine detectionSTAR ProtocolsMarch 1, 2025Gianluca Rotta, Valentina Achille, Scott J. Bornheimer, Melanie Duenas, Marco Fernandez, Arianna Gatti, Sergio Haro Giron, Irene Martinez Rio, Marta Massanella, Diego J. Jiménez, et al.grade",Miltenyi Biotec,130-127-311 "PepTivator™ SARS-CoV-2 Prot_S, research grade",Miltenyi Biotec,130-126-700 "PepTivator™ SARS-CoV-2 Prot_S1, research grade",Miltenyi Biotec,130-127-041 Gibco™ RPMI 1640 medium,Thermo Fisher Scientific

(5) Mass cytometry reveals cellular correlates of immune response heterogeneity to SARS-CoV-2 vaccination in the elderlynpj VaccinesNovember 29, 2024Ratnadeep Mukherjee, Linn Margrethe Eggesbø, Asia-Sophia Wolf, Ingrid Fadum Kjønstad, Guri Solum, Anthony Ravussin, Sabin Bhandari, Anna Hayman Robertson, Per Magnus, Lill Trogstad, et al.and then stimulated for 22 h with either SARS-CoV-2 spike peptides (Wuhan-Hu-1 strain; immunogenic (130-126-700) and complete (130-127-953), 0.3 nM/ml, Miltenyi Biotech, Germany), Cytostim (Miltenyi Biotech, Germany)

(6) Three SARS-CoV-2 spike protein variants delivered intranasally by measles and mumps vaccines are broadly protectiveNature communicationsJuly 3, 2024Zhang Y, Chamblee M, Xu J, Qu P, Shamseldin MM, Yoo SJ, Misny J, Thongpan I, Kc M, Hall JM, et al.(PepTivator SARS-CoV-2 Prot_S1 and PepTivator SARS-CoV-2 Prot_S + ) (Miltenyi Biotec catalog no. 130-126-701& 130-126-700), Peptide pools were used at a final concentration of 1 μg/ml each peptide. Cells incubated with DMSO

(7) Antibody-independent protection against heterologous SARS-CoV-2 challenge conferred by prior infection or vaccinationNature immunologyApril 1, 2024Fumagalli V, Ravà M, Marotta D, Di Lucia P, Bono EB, Giustini L, De Leo F, Casalgrandi M, Monteleone E, Mouro V, et al.peptides (9–22 amino acids) covering the S, S1, S+, M and N proteins of SARS-CoV-2 (Miltenyi Biotec, 130-126-700, 130-127-041, 130-127-311, 130-126-702 and 130-126-698)31. As a positive control for IFN-γ and TNF production,

(8) Association between SARS-CoV-2 RNAemia, skewed T cell responses, inflammation, and severity in hospitalized COVID-19 people living with HIViScienceJanuary 19, 2024Matteo Augello, Valeria Bono, Roberta Rovito, Camilla Tincati, Silvia Bianchi, Lucia Taramasso, Antonio Di Biagio, Annapaola Callegaro, Franco Maggiolo, Elisa Borghi, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, PepTivator® SARS-CoV-2 Prot_S,Miltenyi Biotec,130-126-700 PepTivator® SARS-CoV-2 Prot_N,Miltenyi Biotec,130-126-698 PepTivator® SARS-CoV-2 Prot_M,Miltenyi Biotec,130-126-702 Phorbol

(9) Breakthrough infections by SARS-CoV-2 variants boost cross-reactive hybrid immune responses in mRNA-vaccinated Golden Syrian hamstersPLOS PathogensJanuary 10, 2024Juan García-Bernalt Diego, Gagandeep Singh, Sonia Jangra, Kim Handrejk, Manon Laporte, Lauren A. Chang, Sara S. El Zahed, Lars Pache, Max W. Chang, Prajakta Warang, et al.,Miltenyi Biotec,130-126-698 PepTivator SARS-CoV-2 Prot_S complete research grade (60nmol/peptide),Miltenyi Biotec,130-126-700 Oxoid Agar Bacteriological,Thermo Fisher,LP0011B Phosphate Buffered Saline,Corning,21-040-CMR Penicillin

(10) Sex-biased immunogenicity of a mucosal subunit vaccine against SARS-CoV-2 in miceFrontiers in immunologyJanuary 1, 2024Li J, Hsu KS, Howe SE, Hoang T, Xia Z, Berzofsky JA, Sui Y.Cells were stimulated with 2 μg/mL SARS−CoV−2 PepTivator Peptide Pools (Miltenyi Biotec, catalog # 130–126-700) in the presence of 0.15 μg/mL brefeldin A (BioLegend, catalog # 420601) at 37°C 5% CO2 overnight. The

(11) Immunogenicity and Efficacy of TNX-1800, A Live Virus Recombinant Poxvirus Vaccine Candidate, against SARS-CoV-2 Challenge in Nonhuman PrimatesVaccines (Basel)November 2, 2023Mayanka Awasthi, Anthony Macaluso, Dawn Myscofski, Jon Prigge, Fusataka Koide, Ryan S. Noyce, Siobhan Fogarty, Helen Stillwell, Scott J. Goebel, Bruce Daugherty, et al.control), synthetic peptide pools spanning the SARS-CoV-2 spike protein (Peptivator S Peptide Miltenyi cat#130-126-700, Peptivator S1 Peptide Miltenyi cat#130-127-04 at a final concentration of approximately 1 µg/mL of

(12) BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccineiScienceSeptember 15, 2023Perera DJ, Domenech P, Babuadze GG, Naghibosadat M, Alvarez F, Koger-Pease C, Labrie L, Stuible M, Durocher Y, Piccirillo CA, et al.554723 PepTivator SARS-CoV-2 Prot_S,"Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany",Cat: 130-126-700 PepTivator SARS-CoV-2 Prot_S Complete,Miltenyi Biotec,Cat: 130-127-951 SARS-CoV-2 Neutralization Antibody

(13) Growth hormone-releasing hormone antagonist MIA-602 inhibits inflammation induced by SARS-CoV-2 spike protein and bacterial lipopolysaccharide synergism in macrophages and human peripheral blood mononuclear cellsFrontiers in ImmunologyAugust 15, 2023Giuseppina Granato, Iacopo Gesmundo, Francesca Pedrolli, Ramesh Kasarla, Laura Begani, Dana Banfi, Stefania Bruno, Tatiana Lopatina, Maria Felice Brizzi, Renzhi Cai, et al.Technologies (Thermo Fisher Scientific, Milan, Italy). PepTivator SARS-CoV-2 Spike protein (S protein, code: 130-126-700) was purchased from Miltenyi Biotec (Bologna, Italy). Western blot reagents were purchased from Bio-Rad

(14) Breakthrough infections by SARS-CoV-2 variants boost cross-reactive hybrid immune responses in mRNA-vaccinated Golden Syrian HamstersbioRxivJune 28, 2023Juan García-Bernalt García-Bernalt Diego, Gagandeep Singh, Sonia Jangra, Kim Handrejk, Manon Laporte, Lauren A. Chang, Sara S. El Zahed, Lars Pache, Max W. Chang, Prajakta Warang, et al.,Miltenyi Biotec,130-126-698 PepTivator® SARS-CoV-2 Prot_S complete research grade (60nmol/peptide),Miltenyi Biotec,130-126-700 Oxoid™ Agar Bacteriological,Thermo Fisher,LP0011B Phosphate Buffered Saline,Corning,21–040-CMR Penicillin

(15) Nirmatrelvir treatment of SARS-CoV-2-infected mice blunts antiviral adaptive immune responsesEMBO molecular medicineMay 8, 2023Fumagalli V, Di Lucia P, Ravà M, Marotta D, Bono E, Grassi S, Donnici L, Cannalire R, Stefanelli I, Ferraro A, et al.II‐restricted peptides (9–22 amino acids) covering the S, S1, S+, M, and N protein of SARS‐CoV‐2 (Miltenyi, #130‐126‐700; #130‐127‐041; #130‐127‐311; #130‐126‐702, #130‐126‐698; Silva‐Cayetano et al, 2021). In selected experiments

(16) Prime-Pull Immunization of Mice with a BcfA-Adjuvanted Vaccine Elicits Sustained Mucosal Immunity That Prevents SARS-CoV-2 Infection and PathologyJournal of immunology (Baltimore, Md. : 1950)May 1, 2023Shamseldin MM, Kenney A, Zani A, Evans JP, Zeng C, Read KA, Hall JM, Chaiwatpongsakorn S, Mahesh KC, Lu M, et al.(PepTivator SARS-CoV-2 Prot_S1 and PepTivator SARS-CoV-2 Prot_S+), (Miltenyi Biotec Ref. 130-126-701& 130-126-700) for 5–6 hr at 37°C in the presence of protein transport inhibitor cocktail (eBioscience). Peptide pools

(17) Immunological response after SARS-CoV-2 infection and mRNA vaccines in patients with myasthenia gravis treated with RituximabNeuromuscular DisordersMarch 1, 2023Valentina Damato, Gregorio Spagni, Gabriele Monte, Letizia Scandiffio, Paola Cavalcante, Nicole Zampetti, Marco Fossati, Silvia Falso, Renato Mantegazza, Alessandra Battaglia, et al.and CD8+ T cells, we employed PepTivator SARS-CoV-2 Prot N, S and S1 (Miltenyi Biotec 130–126–698; 130–126–700; 130–127–311 respectively). PBMCs were seeded at a density of 5 × 10⁶ cells/cm² in a 96-multiwell flat-bottom

(18) A high CMV-specific T cell response associates with SARS-CoV-2-specific IL-17 T cell productionMedical Microbiology and ImmunologyFebruary 1, 2023Fernanda Tereza Bovi Frozza, Tiago Fazolo, Priscila Oliveira de Souza, Karina Lima, Julia Crispim da Fontoura, Théo Souza Borba, Márcia Polese-Bonatto, Luciane Beatriz Kern, Renato T. Stein, Graham Pawelec, et al.IE2, and envelope glycoprotein B—Mabtech, Sweden) or 1 µg/mL of peptide PepTivator SARS-CoV-2 Prot S (130–126-700—Miltenyi Biotec, Germany), PepTivator SARS-CoV-2 Prot N (130–126-698—Miltenyi Biotec, Germany) or PepTivator

(19) SARS-CoV-2 mRNA Dual Immunization Induces Innate Transcriptional Signatures, Establishes T-Cell Memory and Coordinates the Recall ResponseVaccines (Basel)January 1, 2023Ioanna Papadatou, Maria Geropeppa, Kleio-Maria Verrou, Marianna Tzanoudaki, Theano Lagousi, Emmanouil Liatsis, Vana Spouloustimulated for 2 h with SARS-CoV-2 S-protein PepTivator® SARS-CoV-2 Prot_S—research grade 6 nmol/peptide (#130-126-700) (Miltenyi, Bergisch Gladbach, Germany), containing a pool of lyophilized peptides, consisting mainly

(20) Robust spike-specific CD4(+) and CD8(+) T cell responses in SARS-CoV-2 vaccinated hematopoietic cell transplantation recipients: a prospective, cohort studyFrontiers in immunologyJanuary 1, 2023Federico L, Tvedt THA, Gainullin M, Osen JR, Chaban V, Lund KP, Tietze L, Tran TT, Lund-Johansen F, Kared H, et al.(PepTivator® SARS-CoV-2 Prot_S Complete; Miltenyi, # 130-127-953), and Spike-I (PepTivator® SARS-CoV-2 Prot_S; Militenyi, # 130-126-700) pools are collections of lyophilized 15-mers peptides with 11 aa overlaps spanning either the immunodominant

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PepTivator® SARS-CoV-2 Prot_S from Miltenyi Biotec

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PepTivator® SARS-CoV-2 Prot_S from Miltenyi Biotec

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