Fig 1: Immature CD10neg neutrophils from patients with AMI express high amounts of MMP-9 and S100A9 and display resistance to apoptosis.(A) May-Grünwald Giemsa stained cytospin preparations of CD16+CD66b+CD10neg (CD10neg) and CD16+CD66b+CD10pos (CD10pos) neutrophils. (B) Linear regression analysis between the percentages of CD16+CD66b+CD10neg neutrophils and circulating levels of G-CSF in patients with acute coronary syndrome (n=71). (C) Mean fluorescence intensity (MFI) of CD11b, CD101, CD114, and CD177 on CD10neg versus CD10pos neutrophils (n=25). (D) Relative RNA expression of MMP-9 and S100A9 in CD10neg versus CD10pos neutrophils. (E) Percentage of apoptotic neutrophils assessed by flow cytometry using Vybrant DyeCycle Violet stain and SYTOX AADvanced stain. CD10neg/CD10pos neutrophils were isolated by flow-cytometric sorting from patients with AMI (n=4–5). *p<0.05.
Fig 2: CD14+HLA-DRneg/low monocytes from patients with AMI are not immunosuppressive but exhibit an inflammatory phenotype.(A) May-Grünwald Giemsa stained cytospin preparations of CD14+HLA-DRneg/low and CD14+HLA-DRhigh monocytes. (B) Relative RNA expression of S100A9 and IL1R1 in CD14+HLA-DRneg/low versus CD14+HLA-DRhigh monocytes. (C) T-cell proliferation in the presence of CD14+HLA-DRneg/low or CD14+HLA-DRhigh monocytes assessed by CellTrace Violet dilution after 96 hr of co-culture. (D) Macrophages differentiated from CD14+HLA-DRneg/low monocytes and (E) CD14+HLA-DRhigh cells by 4-day culture with M-CSF. (F) TNF-a, IL-6, and IL-1ß in supernatants of macrophage cultures upon stimulation with IFN-?. Mb=baseline. CD14+HLA-DRneg/low/CD14+HLA-DRhigh cells were isolated by flow-cytometric sorting from patients with AMI (n=5–6). Data are presented as mean ± SEM. *p<0.05.
Fig 3: Multiple intercorrelations among CD10neg neutrophils, HLA-DRneg/low monocytes and immune-inflammation markers.Spearman-correlation matrix of CD16+CD66b+CD10neg neutrophils (%), CD14+HLA-DRneg/low monocytes (%) and circulating levels of MMP-9, S100A9/S100A8, IL-6, IL-1ß, TNF-a, MPO, and NGAL (levels). UA (n=11), NSTEMI (n=10), and STEMI (n=26). Each circle illustrates a significant correlation between different couples of parameters (p<0.05). The correlation coefficient is colored and sized up according to the value; square leaved blank indicates not significant correlation.
Supplier Page from Thermo Fisher Scientific for Recombinant Human S100A9 Protein