Fig 1: The fluorescent and confocal microscopy images of neurons differentiated from PC12 cells incubated with a primary antibody against neurofilaments and Alexa Fluor 488 as the secondary antibody(A and D) The images were captured in phase contrast.(B and E) Fluorescent microscopy images.(C and F) Merged images of phase contrast and fluorescent microscopy images.(G) The confocal light images of the positive control (PC12 cells treated with NGF).(H) Fluorescent confocal image of the positive control.(I) Merged images of light and fluorescent confocal microscopy images.
Supplier Page from Thermo Fisher Scientific for Recombinant Mouse NGF Protein