Fig 1: Metabolic flow cytometry identifies distinct phenotypes between macrophages in vitro.a Schematic of metabolic targets for flow-based analysis. b Relative change in geometric MFI of metabolic targets by stimulated BMDM relative to media control, and representative histograms below. Data points represent BMDM cultures from individual mice, pooled from 3–4 experiments (n = 12–16) with mean shown. c Fold change of metabolic target expression (gMFI) in GM-CSF relative to M-CSF differentiated human macrophages, datapoints represent individual donors (n = 6–9). Statistics calculated with (b) RM one-way ANOVA with Geisser-Greenhouse correction and Dunnett Test to correct for multiple comparisons, or (c) Wilcoxon two-tailed matched-pairs signed rank test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Source data are provided as a Source Data file.