Recombinant Human VEGF-165 Protein PHC9391 from Thermo Fisher Scientific

Product Specs
ItemRecombinant Human VEGF-165 Protein
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPHC9391
Size100 µg
ApplicationsControl, ELISA, Functional assay, Immunohistochemistry, Western Blot
Formatno preservative
TypeRecombinant
SpeciesHuman
Molecule NameHuman VEGF-165
NCBI Full Gene Namevascular endothelial growth factor A
NCBI Gene AliasesMVCD1, VEGF, VPF
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Somatic cancer variants enriched in Alzheimer's disease microglia-like cells drive inflammatory and proliferative statesCellApril 21, 2026August Yue Huang, Zinan Zhou, Maya Talukdar, Liz Enyenihi, Michael B. Miller, Brian Chhouk, Ila Rosen, Mengyue Zheng, Minye Zhou, Averill Yang, et al.5265-IL MCSF,Biotechne,Cat# 216-MC TGFβ1,Biotechne,Cat# 7754-BH VEGF,Thermo Fisher Scientific,Cat# PHC9391 GlutaMax Supplement,Thermo Fisher Scientific,Cat# 35050061 Insulin,Sigma,Cat # I9278 "DMEM/F-12, HEPES, no phenol red

(2) Assessing Aβ-independent effects of Module 42 on immune function in vitroAlzheimers DementFebruary 25, 2026Ishita Ajith, Souvika Bakshi, Emma Mead, Opher Gileadi, Vittorio L. Katis, Paul E. Brennan, Emory‐Sage‐SGC‐Jax TREAT‐AD Center, Katerina O. Gospodinovamorphogenetic protein‐4; 50 ng/mL, PHC9534, Life Technologies), (Vascular endothelial growth factor; 50 ng/mL, PHC9391, Life Technologies), and (Stem cell factor; 20 ng/mL, 130‐096‐695, Miltenyi Biotec) for 3 days. EBs

(3) Multi-omics analysis reveals metabolic diversity underlying endothelial cell functionsLife Sci AllianceDecember 17, 2025Stephan Durot, Peter F Doubleday, Lydia Schulla, Amélie Sabine, Tatiana V Petrova, Nicola Zamboniwere stimulated with mixed medium containing 350 ng/ml human VEGF-165 recombinant protein (cat. no. PHC9391; Thermo Fisher Scientific), for a final concentration of 50 ng/ml in the gel. Depending on the condition,

(4) AbDesign: database of point mutants of antibodies with associated structures reveals poor generalization of binding predictions from machine learning modelsMAbsOctober 8, 2025Bartosz Janusz, Dawid Chomicz, Samuel Demharter, Marloes Arts, Jurrian de Kanter, Yano Wilke, Helena Britze, Sonia Wrobel, Tomasz Gawłowski, Pawel Dudzic, et al.protein, His tag","AcroBiosystems, Cat# GP4-V15223" Human VEGF-165 Recombinant Protein,"Gibco, Cat# PHC9391" "SIRP alpha Protein, Human, Recombinant (ECD, SIRP alphaV2, His Tag)","SinoBiologicals, Cat# 30014-H08H" Human CTLA-4 His-tag Recombinant Protein

(5) TET2-mutant myeloid cells mitigate Alzheimer’s disease progression via CNS infiltration and enhanced phagocytosis in miceCell Stem CellAugust 7, 2025Katie A. Matatall, Trisha K. Wathan, Minh Nguyen, Hu Chen, Alexandra McDonald, Guantong Qi, Julia A. Belk, Marcus A. Florez, Duy T. Le, Temitope Olarinde, et al.5265-IL MCSF,Biotechne,Cat# 216-MC TGFβ1,Biotechne,Cat# 7754-BH VEGF,Thermo Fisher Scientific,Cat# PHC9391 GlutaMax Supplement,Thermo Fisher Scientific,Cat# 35050061 Insulin,Sigma,Cat # I9278 "DMEM/F-12, HEPES, no phenol red

(6) c-Src-induced vascular malformations require localised matrix degradation at focal adhesionsJournal of cell scienceJuly 1, 2024Essebier P, Keyser M, Yordanov T, Hill B, Yu A, Noordstra I, Yap AS, Stehbens SJ, Lagendijk AK, Schimmel L, et al.overnight in EBM-Plus with 1% FBS prior to addition of 100 ng/ml VEGF-A165 (Thermo Fisher Scientific, PHC9391, in 0.1% BSA in PBS) in starvation medium. For EdU cell proliferation assay, cells were incubated for

(7) Choroid plexus defects in Down syndrome brain organoids enhance neurotropism of SARS-CoV-2Science advancesJune 7, 2024Shaker MR, Slonchak A, Al-Mhanawi B, Morrison SD, Sng JDJ, Cooper-White J, Khromykh AA, Wolvetang EJ.(Sigma-Aldrich, catalog no. SML1046-5MG), and BMP4 (2 ng/ml) (Thermo Fisher Scientific, catalog no. PHC9391). Patterned neural spheroids were then embedded in Matrigel (STEMCELL Technologies, catalog no. 354277)

(8) Vorolanib, sunitinib, and axitinib: A comparative study of vascular endothelial growth factor receptor inhibitors and their anti-angiogenic effectsPLOS ONEJune 4, 2024Sophie J. Bakri, Jeff Lynch, Michelle Howard-Sparks, Stephan Saint-Juste, Said Saimsponge (BloXang, Bausch + Lomb) was administered on the CAM and loaded with 100 ng (10 μL) of VEGF-165 (PHC9391, ThermoFischer). The window was sealed with tape, and eggs were returned to the incubator. At day 9

(9) Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprintingLab on a chipMay 14, 2024Shrestha S, Lekkala VKR, Acharya P, Kang SY, Vanga MG, Lee MY.recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system; 236-EG), 0.5 μM A 83-01 (R&D Systems; 2939), 50 μg/mL

(10) Heparin‐modified alginate microspheres enhance neovessel formation in hiPSC ‐derived endothelial cells and heterocellular in vitro models by controlled release of vascular endothelial growth factor Journal of Biomedical Materials Research Part ASeptember 1, 2021Fabiola Munarin, Carly Kabelac, Kareen L.K. Coulombe2-morpholinoethane sulfonic acid (Sigma M3671). For in vitro and ex vivo assays, VEGF (Life Technologies, PHC9391) was reconstituted in 0.1% bovine serum albumin (BSA) and used without further modifications. iCell®

(11) Capturing Cardiogenesis in GastruloidsCell stem cellFebruary 4, 2021Rossi G, Broguiere N, Miyamoto M, Boni A, Guiet R, Girgin M, Kelly RG, Kwon C, Lutolf MP.

(12) CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fatStem cells translational medicineNovember 1, 2020Borrelli MR, Patel RA, Blackshear C, Vistnes S, Diaz Deleon NM, Adem S, Shen AH, Sokol J, Momeni A, Nguyen D, et al.separate wells of endothelial cells were treated with 10 ng/mL recombinant VEGF (ThermoFisher, Cat#PHC9391) alone as a positive control. Endothelial‐ACS cultures were incubated for 18 hours and then the endothelial cells

(13) Endothelial Cells Derived From Patients With Diabetic Macular Edema Recapitulate Clinical Evaluations of Anti-VEGF Responsiveness Through the Neuronal Pentraxin 2 PathwayDiabetesOctober 1, 2020Marta Vilà González et al.replaced with StemPro™-34 SFM (10639011, Thermo Fisher Scientific) supplemented with 200 ng/ml VEGF (PHC9391, Thermo Fisher Scientific) and 2 μM forskolin (F6886, SIGMA). This step was repeated after 24 hours. On

(14) Targeting QKI-7 in vivo restores endothelial cell function in diabetesNature communicationsJuly 30, 2020Yang C, Eleftheriadou M, Kelaini S, Morrison T, González MV, Caines R, Edwards N, Yacoub A, Edgar K, Moez A, et al.34 medium (Thermo Fisher Scientific 10639011) supplemented with 200 ng/ml hVEGF (Life Technologies PHC9391) and 2 µM Forskolin (Sigma-Aldrich F6886). On day 6, CD144 positive cells were magnetically sorted using

(15) Human Umbilical Cord Mesenchymal Stem Cell Differentiation Into Odontoblast-Like Cells and Endothelial Cells: A Potential Cell Source for Dental Pulp Tissue EngineeringFrontiers in physiologyJanuary 1, 2020Zhang S, Zhang W, Li Y, Ren L, Deng H, Yin X, Gao X, Pan S, Niu Y.

(16) Monocarbonyl curcumin analog A2 potently inhibits angiogenesis by inducing ROS-dependent endothelial cell deathActa pharmacologica SinicaNovember 1, 2019Liu B, Cui LS, Zhou B, Zhang LL, Liu ZH, Zhang L.

(17) Sustained Anti-Vascular Endothelial Growth Factor Activity of Aflibercept (Eylea) After Storage in Polycarbonate Syringes Used for Intravitreal Injection: A Pathway to Safety and EfficiencyJournal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and TherapeuticsJune 1, 2019Han DP, Skumatz C, Besharse JC, Kassem IS.phosphate-buffered saline (PBS) to increase sensitivity of the experiment. Recombinant human VEGF (rhVEGF, cat no. PHC9391; Fisher Scientific) was diluted to 100 ng/mL with PBS containing 0.1% bovine serum albumin (BSA) and

(18) iPSC-Derived Human Microglia-like Cells to Study Neurological DiseasesNeuronApril 19, 2017Abud EM, Ramirez RN, Martinez ES, Healy LM, Nguyen CHH, Newman SA, Yeromin AV, Scarfone VM, Marsh SE, Fimbres C, et al.PHC0061 Human IL3,Thermo Fisher Scientific,Cat# PHC0031 Human VEGF,Thermo Fisher Scientific,Cat# PHC9391 Human SCF,Thermo Fisher Scientific,Cat# PHC2111 Human Fractalkine (CX3CL1),Peprotech,Cat# 300-31 Human

(19) The docking protein FRS2α is a critical regulator of VEGF receptors signalingProceedings of the National Academy of Sciences of the United States of AmericaApril 15, 2014Chen PY, Qin L, Zhuang ZW, Tellides G, Lax I, Schlessinger J, Simons M.matrigel plug assay. Growth factor reduced Matrigel (354230; BD Biosciences) were premixed with VEGF-A165 (PHC9391, Invitrogen) and heparin (20 U/mL). The samples were injected into the s.c. tissues of 10-wk-old mice. Once

(20) Clinical impact of pentraxin family expression on prognosis of pancreatic carcinomaBritish journal of cancerAugust 6, 2013Kondo S, Ueno H, Hosoi H, Hashimoto J, Morizane C, Koizumi F, Tamura K, Okusaka T.medium in the upper chamber at 1 × 105 cells ml−1, and the bottom chamber contained VEGF (Cat. no. PHC9391; Life Technologies Corp.), PTX3 (Cat. no. 1826-TS-025; R&D System, Minneapolis, MN, USA), and CRP (Cat.

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Recombinant Human VEGF-165 Protein from Thermo Fisher Scientific

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Recombinant Human VEGF-165 Protein from Thermo Fisher Scientific

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