bFGF-target and off-target gene expression was comparable in cells treated with native bFGF and Heat Stable bFGF. The TaqMan Array Human Signal Transduction Pathways array (Product # 4418775) and the TaqMan Array Human FGF Pathway (Product # 4414136) were used to assess expression of genes in the androgen, calcium, CREB, estrogen, hedgehog, insulin, JAK-STAT, LDL, mitogenic, NFAT, NFκB, p53, phospholipase C, protein kinase C, retinoic acid, stress, survival, TGF-β Wnt, and FGF-associated pathways, respectively. Expression was assessed using human induced pluripotent stem cells treated with 10 ng/mL native bFGF or HS bFGF. Genes from both arrays were separated into 'bFGF-target' or 'off-target' based on their downstream relationship to bFGF, as is currently indicated by literature. The bFGF-target and off-target panels show the results from 43 and 71 genes, respectively.
Supplier Page from Thermo Fisher Scientific for Recombinant Human Heat Stable bFGF Protein