Recombinant Human TGF-beta 1 Protein PHG9214 from Thermo Fisher Scientific

Product Specs
ItemRecombinant Human TGF-beta 1 Protein
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPHG9214
Size10 µg
ApplicationsControl, ELISA, Functional assay, Immunohistochemistry, Western Blot
Format100mM acetic acid with no preservative
TypeRecombinant
SpeciesHuman
Molecule NameHuman TGF-beta 1
NCBI Full Gene Nametransforming growth factor beta 1
NCBI Gene AliasesCED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta
Storage4° C

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) miR-424(322)~503 impairs colon cancer progression driven by PTEN deficiencyCell Death & DiseaseFebruary 24, 2026Maria Vidal-Sabanés, Núria Bonifaci, Raúl Navaridas, Joaquim EGEA, Mario Encinas, Ruth Rodriguez-Barrueco, Jose M. Silva, Xavier Matias-Guiu, Jordi Tarragona, David Llobet-Navas, et al.were completely formed. Organoid treatments were performed two days after medium replacement. TGF-β (PHG9214, Gibco) treatments were performed at 10 ng/μl for 16 h. miRNA extraction and real-time qPCR miRNA

(2) Endometrial cancer progression driven by PTEN-deficiency requires miR-424(322)~503Cell Death DisOctober 6, 2025Maria Vidal-Sabanés, Núria Bonifaci, Raúl Navaridas, Joaquim Egea, Mario Encinas, Ruth Rodriguez-Barrueco, Jose M. Silva, Xavier Matias-Guiu, David Llobet-Navas, Xavier Dolcetwere completely formed. Organoid treatments were performed two days after medium replacement. TGF-β (#PHG9214, Gibco) treatments were performed at the indicated doses and times. Whole genome miRNA sequencing TruSeq

(3) Response splicing quantitative trait loci in primary human chondrocytes identify putative osteoarthritis risk genesNature communicationsAugust 26, 2025Byun S, Shine J, Coryell P, Kramer NE, D'Costa S, Thulson E, Parkus SM, Chubinskaya S, Loeser RF, Diekman BO, et al.as passage 1 cells, then cultured in media supplemented with 1 ng/mL TGF-β1 (Life Technologies, cat PHG9214) and 5 ng/mL bFGF (Life Technologies, cat PHG0264) to expand. All 46 selected single colonies were confirmed

(4) Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironmentiScienceApril 18, 2025Maximilian Wess, Manuel Rogg, Constance Gueib-Picard, Annika Merz, Anna L. Kössinger, Tobias Feilen, Grigor Andreev, Martin Werner, Ian J. Frew, Markus Grabbert, et al.supplemented with 10% FCS. TGFβ treatment was performed via addition of 5 ng/mL recombinant human TGFβ (PHG9214, Thermo Fisher Scientific Inc., Waltham, MA, USA) for 24h until harvesting of the cells for further

(5) Generation of self-renewing neuromesodermal progenitors with neuronal and skeletal muscle bipotential from human embryonic stem cellsCell Rep MethodsNovember 18, 2024Sun P, Yuan Y, Lv Z, Yu X, Ma H, Liang S, Zhang J, Zhu J, Lu J, Wang C, et al.Human FGF-basic (FGF-2/bFGF) Recombinant Protein,Invitrogen,Cat# PHG0263 Human TGF-beta 1 Recombinant Protein,Invitrogen,Cat# PHG9214 Recombinant Human EGF,PeproTech,Cat# AF-100-15 Recombinant Human HGF,PeproTech,Cat# 100-39 Recombinant Human IGF-I

(6) Matricellular protein CCN1 promotes collagen alignment and scar integrity after myocardial infarctionMatrix BiologyNovember 1, 2024Annalara G. Fischer, Erin M. Elliott, Kenneth R. Brittian, Lauren Garrett, Ghazal Sadri, Julia Aebersold, Richa A. Singhal, Yibing Nong, Andrew Leask, Steven P. Jones, et al.treated with varying concentrations (ranging from 1 to 20 ng/mL) of recombinant TGFβ1 (Life Technologies; PHG9214) or equivalent concentrations of bFGF (Peprotech; 100–18B) as a control, all in DMEM/F12 base medium.

(7) Comparison of wild-type and high-risk PNPLA3 variants in a human biomimetic liver microphysiology system for metabolic dysfunction-associated steatotic liver disease precision therapyFrontiers in Cell and Developmental BiologySeptember 11, 2024Mengying Xia, Mahboubeh Varmazyad, Iris Pla-Palacín, Dillon C. Gavlock, Richard DeBiasio, Gregory LaRocca, Celeste Reese, Rodrigo M. Florentino, Lanuza A. P. Faccioli, Jacquelyn A. Brown, et al.(Cayman Chemical, 29558), and molecular drivers of disease, including TGF-β1 (Thermo Fisher Scientific, PHG9214) and lipopolysaccharide (Sigma-Aldrich, L2654), as shown in Supplementary Table S1. 96-well plate LAMPS

(8) TSPYL1 as a Critical Regulator of TGFβ Signaling through Repression of TGFBR1 and TSPYL2Advanced ScienceJune 1, 2024Huiqi Tan, Mia Xinfang Miao, Rylee Xu Luo, Joan So, Lei Peng, Xiaoxuan Zhu, Eva Hin Wa Leung, Lina Zhu, Kui Ming Chan, Martin Cheung, et al.primers, and antibodies used in this study were listed in Tables S2–S6 (Supporting Information). TGFβ1 (PHG9214), Pierce Protease Inhibitor Tablets, EDTA‐free (A32965), Halt Protease and Phosphatase Inhibitor Single‐Use Cocktail (100X

(9) Wnt5a/β-catenin-mediated epithelial-mesenchymal transition: a key driver of subretinal fibrosis in neovascular age-related macular degenerationJ NeuroinflammationMarch 26, 2024Dandan Liu, Jingxiao Du, Hai Xie, Haibin Tian, Lixia Lu, Chaoyang Zhang, Guo-Tong Xu, Jingfa Zhang(Wuxi, China). Recombinant transforming growth factor beta 1 (TGFβ1) was purchased from ThermoFisher (PHG9214, Shanghai, China) and was used at the final concentration of 10 ng/mL. FH535 was obtained from MedChemExpress

(10) TGF‐β1 Inhibits Osteoclast Differentiation and Abnormal Angiogenesis in Intervertebral Disc Degeneration: Evidence from RNA Sequencing and Animal StudiesOrthopaedic SurgeryJanuary 1, 2024Keping Wang, Zuolong Wu, Chaoyang Gong, Guanghai Zhao, Haihong Zhanggroup (n = 15). During IDD modeling, the IDD + TGF‐β1 group mice were injected with 0.5 μg of TGF‐β1 (PHG9214, Invitrogen, Thermo Fisher) daily in the IVD, while the TGF‐β1 + Vegf mice were additionally injected

(11) Modulating mechanobiology as a therapeutic target for synovial fibrosis to restore joint lubricationOsteoarthritis and CartilageJanuary 1, 2024Edward D. Bonnevie, Carla R. Scanzello, Robert L. Mauckpenicillin/streptomycin/fungizone, 1.25 mg/mL bovine serum albumin, with or without 10 ng/mL TGFβ1 (ThermoFisher, PHG9214). In vitro imaging and analysis Immunofluorescence imaging for αSMA and YAP/TAZ was conducted on synovial

(12) Circular RNA circSMAD4 regulates cardiac fibrosis by targeting miR-671-5p and FGFR2 in cardiac fibroblastsMolecular Therapy - Nucleic AcidsDecember 12, 2023Anna Jeong, Yongwoon Lim, Taewon Kook, Duk-Hwa Kwon, Young Kuk Cho, Juhee Ryu, Yun-Gyeong Lee, Sera Shin, Nakwon Choe, Yong Sook Kim, et al.ventricular cardiac fibroblasts were starved in FBM for 16 h followed by treatment with human TGF-β1 (#PHG9214, Gibco, MA, USA) for 24 h. Vector constructs The whole length of circRNA was cloned into the overexpression

(13) Chemotherapeutic Activity of Imidazolium-Supported Pd(II) o-Vanillylidene Diaminocyclohexane Complexes Immobilized in Nanolipid as Inhibitors for HER2/neu and FGFR2/FGF2 Axis Overexpression in Breast Cancer CellsPharmaceuticals (Basel, Switzerland)December 11, 2023Awaji AA, Rizk MA, Alsaiari RA, Alqahtani NF, Al-Qadri FA, Alkorbi AS, Hafez HS, Elshaarawy RFM.USA, Cat#EHERBB2), FGFR2 (Cat# 12828 cell signaling technology, USA), TGFβ1 (Thermo Fisher USA, Cat#PHG9214), and P53 (Novus Biologicals, Centennial, CO, USA, NB200-103). An ELISA was carried out following the

(14) Mouse Memory CD8 T Cell Subsets Defined by Tissue-Resident Memory Integrin Expression Exhibit Distinct Metabolic ProfilesImmunoHorizonsOctober 1, 2023Sportiello M, Poindexter A, Reilly EC, Geber A, Lambert Emo K, Jones TN, Topham DJ.TRM-like culture media with 10 ng/ml mouse IL-7 (Cat. 577804; BioLegend) and 10 ng/ml human TGF-β1 (PHG9214; Fisher Scientific) for 6 d. Additional glucose (Cat. A2494001; Fisher Scientific) and lipid supplement

(15) Identification of a comprehensive alternative splicing function during epithelial-mesenchymal transitioniScienceApril 21, 2023Qiu Y, Zhang Y, Tian L, Zou Q, Zhao P.final concentration of 100 ng/mL. MCF10A cells were treated for 3 days with TGF-β1 (5 or 10 ng/mL, PHG9214, Life Technologies, Beijing, China). Method details Data collections We evaluated the performance

(16) MicroRNA-494 augments fibrotic transformation of human retinal pigment epithelial cells and targets p27 with cell-type specificityFront Ophthalmol (Lausanne)April 14, 2023Theodore Leng, Georgia Kamboj, Xiaoyun Sun, Heather Chang, Prisha Davda, Majesty Greer, Creed M. Starytransformed into a fibroproliferative state via incubation with 10 ng/ml recombinant human TGFβ1 (#PHG9214, Thermofisher Scientific, Waltham, MA) for 72h. In parallel experiments Müller and RPE cultures were

(17) The Foundational Data Initiative for Parkinson Disease: Enabling efficient translation from genetic maps to mechanismCell GenomMarch 8, 2023Bressan E, Reed X, Bansal V, Hutchins E, Cobb MM, Webb MG, Alsop E, Grenn FP, Illarionova A, Savytska N, et al.(C24II) N-Terminus),R&D,1845-SH TGFβ1 (recombinant human transforming growth factor-beta 1),Gibco,PHG9214 TGFβ3 (recombinant human transforming growth factor-beta 3),R&D,243-B3 UltraPure 0.5M EDTA,Invitrogen,15575020 Y-27632,Cayman,1000558310 ,, Critical

(18) Upregulation of PD-L1 by SARS-CoV-2 promotes immune evasionJournal of medical virologyFebruary 1, 2023Huang HC, Wang SH, Fang GC, Chou WC, Liao CC, Sun CP, Jan JT, Ma HH, Ko HY, Ko YA, et al.HP DNA Transfection Reagent (6366236001; Roche). After 6 h, cells were treated with 10 ng/ml TGF‐β (PHG9214; Fisher Scientific), 10 ng/ml IL‐1β (PHC0811; Fisher Scientific), 10 ng/ml IL‐6 (PHC0061; Fisher Scientific),

(19) Blocking connexin 43 hemichannel-mediated ATP release reduces communication within and between tubular epithelial cells and medullary fibroblasts in a model of diabetic nephropathyDiabetic medicine : a journal of the British Diabetic AssociationDecember 1, 2022Williams BM, Cliff CL, Demirel I, Squires PE, Hills CE.membranes, buffers and equipment were obtained from Licor Biosciences (Lincoln, USA). Recombinant TGFβ1 (PHG9214) was from Fisher Scientific (Loughborough, UK), while Tonabersat was purchased from Cambridge Bioscience

(20) Regulation of TGF-β1-Induced EMT by Autophagy-Dependent Energy Metabolism in Cancer CellsCancersOctober 4, 2022Hwang JS, Lai TH, Ahmed M, Pham TM, Elashkar O, Bahar E, Kim DR.Pierce BCA Protein Assay Kit, RIPA Lysis and Extraction buffer (89900), and TGF-β1 recombinant Human (INV-PHG9214) were from Thermo Scientific (Waltham, MA, USA); Seahorse XF Real-Time ATP Rate Assay Kit (103592-100),

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Recombinant Human TGF-beta 1 Protein from Thermo Fisher Scientific

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Recombinant Human TGF-beta 1 Protein from Thermo Fisher Scientific

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