Fig 1: LLL12 inhibits IL-6 and LIF induced STAT3 phosphorylation.(A) D283 and (B) U373 cells were pretreated with LLL12 for 2 hours and then treated with IL-6 for 30 minutes to induce p-STAT3. LLL12 was able to block STAT3 activation by IL-6 and did not have an effect of p-AKT or p-ERK. (C) D283 cells were pretreated with LLL12 for 2 hours and then treated with LIF for 24 hours to activate STAT3. LLL12 was able to inhibit the phosphorylation of STAT3 induced by LIF and did not inhibit p-AKT or p-ERK expression.
Fig 2: IL-6 secretion in medulloblastoma and glioblastoma cell lines.IL-6 secretion was measured by ELISA after 48 hours. The cell lines UW288-1, UW426 and U87Δ secreted elevated levels of IL-6.
Fig 3: LLL12 inhibition of IL-6 secretion.(A–C) Cells were treated with LLL12 for 8, 16 and 24 hours and IL-6 levels were measured using an ELISA. IL-6 secretion was reduced at all three time points. (D) LLL12 inhibits the expression of IL-6 mRNA.
Supplier Page from Thermo Fisher Scientific for Recombinant Human IL-6 Protein