Fig 1: Inhibition of CXCR6 limits proinflammatory SASP in senescent HSCs. (a) qPCR analysis of proinflammatory SASP mRNA levels (IL1A, IL1B, IL6, and IL8) in CXCR6-depleted LX-2 cells (n = 3 per group). (b) ELISA of IL-1α secretion (n = 3 per group). (c) WB analysis of NF-κB signaling proteins. (d) qPCR analysis of IL1A, IL1B, IL6, and IL8 in CXCR6-inhibited LX-2 cells treated with rIL-1α and the NF-κB inhibitor PDTC (n = 3 per group). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.