Recombinant Human TNF-alpha Protein PHC3011 from Thermo Fisher Scientific

Product Specs
ItemRecombinant Human TNF-alpha Protein
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPHC3011
Size100 µg
ApplicationsControl, ELISA, Functional assay, Immunohistochemistry, Western Blot
Formatno preservative
TypeRecombinant
SpeciesHuman
Molecule NameHuman TNF-alpha
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21†Biology of ReproductionApril 13, 2026Yuliya Klymenko, Jessica L Kersey, Hunter D Quigley, Shannon M Hawkinsin serum-free media overnight. Cells were treated with 15 ng/ml tumor necrosis factor-alpha (TNF-α; #PHC3011, Gibco) or vehicle (DI water) for 24 h. Steroid hormonal treatment For two-dimensional (2D) experiments,

(2) A deep learning approach to assess transendothelial cell trafficking performanceSci RepApril 3, 2026Thomas Michael Schumacher, Elisabeth Marie Gottloeber, Eric Koziel, Mehmet Sacma, Kaya Eichhorn, Luana Raiber, Johann Gout, Jessica Lindenmayer, Elodie Roger, Michael Karl Melzer, et al.replaced with growth medium containing TNF-α (Gibco, ThermoFisher Scientific, Grand Island, NY, USA, #PHC3011) at 10 ng/ml following incubation for 24 h at 37 °C with 5 % CO2 prior to flow-assay. The TNF-α containing

(3) High‐Content SRS Imaging Unveils Altered Cholesterol Metabolism in Ovarian Cancers Under CAR‐T TreatmentAdvanced ScienceFebruary 15, 2026Chinmayee V. Prabhu Dessai, Zhuoying Huang, Haonan Lin, Guangrui Ding, Hongjian He, Menna Siddiqui, Meng Zhang, Wilson Wong, Ji‐Xin Chengsupplemented with 100 IU/mL IL‐2 (NCI BRB Preclinical Repository), 10 ng/mL TNF‐𝛼 (Thermo Fisher Scientific, PHC3011), 5 ng/mL IFN‐𝛾 (Thermo Fisher Scientific, PHC4033) respectively for 24 h. The cells were then fixed

(4) Beyond antipsychotic efficacy and toward an individualized therapeutic strategy: analysis of the systemic, metabolic, and inflammatory effects of olanzapine, haloperidol, and their combination in schizophrenic patientsFrontiers in PharmacologyFebruary 3, 2026Nayef Samah Alharbi, Noha Alaa Hamdy, Esam M. Aboubakr, Mansour Alharbi, Mostafa A. Ali, Ghaleb Alharbi, Ahmed Ibrahim ElMallah1:300), eBioscience, Thermosientific, USA, and Human TNF-α Recombinant Protein (MW: 17.5 kDa), Catalog #PHC3011 (dilution 1:500), (Gibco, Thermosientific, USA). The membrane was examined at 340 nm using the Analytik

(5) CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistanceCell Reports MedicineJanuary 20, 2026Alex Watterson, Gabriele Picco, Vivien Veninga, Youhani Samarakoon, Chiara M. Cattaneo, Sara F. Vieira, Emre Karakoc, Shriram Bhosle, Thomas W. Battaglia, Sarah Consonni, et al.proteins" ,, IFN-γ,Thermo Fisher,Cat.# 300-02-100 μg IFN-β,STEMCELL Technologies,Cat.# 78113.1 TNF-α,Thermo Fisher,Cat.# PHC3011 IL-6,Thermo Fisher,Cat.# PHC0064 Basement membrane extract (BME),R&D Systems,Cat.# 3433-010-R1 Fixable

(6) Decoding necrosome assembly: harmonizing signal amplification and attenuation through optimal RIP3 stoichiometryNature CommunicationsDecember 23, 2025Xiang Li, Yating Cao, Fei Xu, Yiting Zhang, Yue Kong, Chengjie Lan, Rongfeng Zhu, Cheng Lin, Chuan-Qi Zhong, Zhilong Liu, et al.Qinxi Li at Xiamen University). All plasmids were verified by DNA sequencing. Recombinant human TNF-α (PHC3011) was from Thermo Fisher Scientific and used at 0.1–10 ng/mL. The following compounds were used: Smac

(7) Comparing the Metabolic, Systemic, and Neuropsychiatric Impacts of Olanzapine and Clozapine in Patients with SchizophreniaPharmaceuticals (Basel)September 1, 2025Nayef Samah Alharbi, Noha Alaa Hamdy, Esam M. Aboubakr, Mansour Alharbi, Mostafa A. Ali, Ghaleb Alharbi, Ahmed Ibrahim ElMallaheBioscience, Thermo Scientific, Waltham, MA, USA), and Human TNF-α Recombinant Protein (MW: 17.5 kDa; catalog #PHC3011; dilution 1:500; Gibco, Thermo Scientific, Waltham, MA, USA) were used to determine the IL-6, IL-1β,

(8) Regulation of p65 nuclear localization and chromatin states by compressive forceMolecular Biology of the CellApril 1, 2025Rajshikhar Gupta, Paulina Schärer, Yawen Liao, Bibhas Roy, Roger M. Benoit, G. V. Shivashankarcompressive force (Material and Methods) for 60 min. Recombinant TNFα (Life Technologies, Catalogue No. PHC3011) was added to cell culture media to create 20 ng/ml solution. The HMF3A cells cultured in a 3D collagen

(9) Histidine-rich glycoprotein inhibits TNF-α–induced tube formation in human vascular endothelial cellsFrontiers in PharmacologyMarch 21, 2025Omer Faruk Hatipoglu, Takashi Nishinaka, Kursat Oguz Yaykasli, Shuji Mori, Masahiro Watanabe, Takao Toyomura, Masahiro Nishibori, Satoshi Hirohata, Hidenori Wake, Hideo Takahashiconditions. 2 Materials and methods 2.1 Reagents and antibodies TNF-α was purchased from Gibco (PHC3011, Waltham, MA, United States). The integrin alpha V antibody was obtained from R&D Systems (AF1219-SP,

(10) Simple, streamlined, cost-effective cDNA synthesis method from cell culturesOpen BiolMarch 12, 2025Daniel Stránský, Monika Šteigerová, Markéta Kuklová, Veronika Hanzíková, Nikolina Canová, Jiří Novotný, Ladislav Šenolt, Ondřej Slanař(Sigma-Aldrich; L6529) in the final concentration of 100 ng ml−1 or TNFα (Thermo Fisher Scientific; PHC3011) in final concentrations of 1, 10 or 100 ng ml−1. After 6 h of incubation, cultivation was terminated,

(11) Sevoflurane pretreatment alleviates hypoxia-reoxygenation-induced myocardial cell injury by upregulating miR-21-5pFrontiers in cardiovascular medicineJanuary 1, 2025Qiu S, Chen H, Jiang Q.China), H-FABP (ab108842, Abcam, Shanghai, China), IL-1β (KE00021, Proteintech, Wuhan, China), TNF-α (PHC3011, Thermo Fisher Scientific Inc, Rockford, Illinois, USA), IL-6 (88-7066-88, Thermo Fisher Scientific

(12) Cytokine screening identifies TNF to potentially enhance immunogenicity of pediatric sarcomasFrontiers in ImmunologyDecember 11, 2024Hendrik Gassmann, Melanie Thiede, Jennifer Weiß, Emilie Biele, Luisa Flohé, Helena Lachermaier, Carolin Prexler, Valentina Evdokimova, Laszlo Radvanyi, Irfan Akhtar, et al.treated with 10 ng/ml IL-1β (#IL038, Millipore), 10 ng/ml IL-6 (#GF338, Millipore) or 10 ng/ml TNF (#PHC3011, Thermo Fisher), or 10 ng/ml IFNα (#8927, Cell Signaling) as positive control. Where indicated, EwS

(13) Unveiling impaired vascular function and cellular heterogeneity in diabetic donor-derived vascular organoidsStem CellsSeptember 10, 2024Naderi-Meshkin H, Wahyu Setyaningsih WA, Yacoub A, Carney G, Cornelius VA, Nelson CA, Kelaini S, Donaghy C, Dunne PD, Amirkhah R, et al.concentration (33 mM, adapted from our previous publication11), plus 1 ng/mL human TNF [Invitrogen, PHC3011] and 1 ng/mL IL-6 [Invitrogen, PHC3011]). As a control, to maintain the same osmotic pressure in ND-VOs

(14) Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assembliesCellJuly 11, 2024Natasha I. Edman, Ashish Phal, Rachel L. Redler, Thomas Schlichthaerle, Sanjay R. Srivatsan, Devon Duron Ehnes, Ali Etemadi, Seong J. An, Andrew Favor, Zhe Li, et al.coverslips coated with 0.1% gelatin, following which they were treated with 10 ng/mL TNF-a [Gibco, #PHC3011] in DMEM low glucose (1 g/L) for 24 hours. After 24 hours, the cells were fixed with 4% paraformaldehyde

(15) Anthrax lethal toxin and tumor necrosis factor-α synergize on intestinal epithelia to induce mouse deathProtein & CellFebruary 1, 2024Xinhe Gao, Teng Teng, Yifei Liu, Tingting Ai, Rui Zhao, Yilong Fu, Peipei Zhang, Jiahuai Han, Yingying ZhangUSA), 100 units/mL of penicillin, and 100 μg/mL of streptomycin. 2 μg/mL of LT, 30 ng/mL of human TNF (PHC3011, Gibco), and 10 μmol/L of LCL-161 were used to induce cell death in HT-29 cells. Inhibitors used in

(16) Extracellular Matrix Protein-1 as a Mediator of Inflammation-Induced Fibrosis After Myocardial InfarctionJACC: Basic to Translational ScienceDecember 1, 2023Sean A. Hardy, Laura Liesinger, Ralph Patrick, Maria Poettler, Lavinia Rech, Juergen Gindlhuber, Nishani S. Mabotuwana, DiyaaEldin Ashour, Verena Stangl, Mark Bigland, et al.concentrations: ECM-1 (20 ng/mL; R&D systems; catno. 3937-EC), and TNF-α (20 ng/mL; Gibco; cat no. PHC3011). Protein and mRNA were then extracted from cells (Supplemental Methods). Alternatively, MTT Cell Proliferation

(17) Bispecific soluble cytokine receptor-nanobody fusions inhibit Interleukin (IL-)6 trans-signaling and IL-12/23 or tumor necrosis factor (TNF) signalingThe Journal of biological chemistryNovember 1, 2023Gesiorowski A, Ettich J, Werner J, Wittich C, Pieper S, Padrini G, Behnke K, Floss DM, Lang PA, Moll JM, et al.(Thermo Fisher Scientific, cat. #15452969). Afterwards, 1 ng/ml hTNFα (Thermo Fisher Scientific, cat. # PHC3011) was added with or without indicated concentrations of cs130-TNFVHHFc, TNFVHHFc, and Etanercept and

(18) N,N-Dimethylglycine Sodium Salt Exerts Marked Anti-Inflammatory Effects in Various Dermatitis Models and Activates Human Epidermal Keratinocytes by Increasing Proliferation, Migration, and Growth Factor ReleaseInternational Journal of Molecular SciencesJuly 9, 2023Alexandra Lendvai, Gabriella Béke, Erika Hollósi, Maike Becker, Jörn Michael Völker, Erik Schulze zur Wiesche, Attila Bácsi, Tamás Bíró, Johanna MihályIFNγ recombinant proteins were purchased from Thermo Fisher Scientific (Waltham, MA, USA; Cat. No. PHC3011 and PHC4031), whereas Poly-IC was bought from Merck KGaA (Darmstadt, Germany; Cat. No. P0913). All agents

(19) Vascular Regulation by Super Enhancer-Derived LINC00607Frontiers in Cardiovascular MedicineJune 28, 2022Kiran Sriram, Yingjun Luo, Dongqiang Yuan, Naseeb Kaur Malhi, Alonso Tapia, Vishnu Amaram Samara, Rama Natarajan, Zhen Bouman Chenhigh glucose (25 mM final concentration) + TNFα treatment (5 ng/ml; Thermo Fisher Scientific Cat # PHC3011) (abbreviated as HT) for 3 days as in our previous study (28). For the reversal experiment, ECs were

(20) NPT1220-312, a TLR2/TLR9 Small Molecule Antagonist, Inhibits Pro-Inflammatory Signaling, Cytokine Release, and NLRP3 Inflammasome ActivationInternational Journal of InflammationFebruary 27, 2022Agata Habas, Srinivasa Reddy Natala, Jon K. Bowden-Verhoek, Emily M. Stocking, Diana L. Price, Wolfgang Wrasidlo, Douglas W. Bonhaus, Martin B. Gillwere purchased from InvivoGen (San Diego, CA USA). Recombinant human tumor necrosis factor-α (TNFα, PHC3011) was purchased from Fisher Scientific. TLR9 inhibitor (E6446) was purchased from Selleckchem (Houston,

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Recombinant Human TNF-alpha Protein from Thermo Fisher Scientific

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Recombinant Human TNF-alpha Protein from Thermo Fisher Scientific

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