Fig 1: The effects of estrogen receptor beta (EsRb) gene knockdown on mRNA expression of IL3RA, estrogen receptor alpha (EsRa), MMPs, and COL11A2 in chondrocytes from human females. Fold change in gene expression in each knockdown (KD) group compared with that in the non-KD control was determined using qPCR. For each group, the assay was performed in triplicate, and chondrocytes were treated with 10 nM of IL-3. The effects are presented for the 10 nM estrogen groups. n = 3 each group. * p < 0.05, ** p < 0.01. Independent t-test was used for statistical analysis. The values are shown as mean with a 95% confidence interval (CI).
Fig 2: The effects of IL3RA knockdown on mRNA expression of MMPs and COL11A2 in chondrocytes from human males. Fold change in gene expression in each knockdown (KD) group compared with that in the non-KD control was determined using qPCR. For each group, the assay was performed in triplicate, and chondrocytes were treated with 10 nM of IL-3. The effects are presented for different concentrations of testosterone groups. n = 3 each group. * p < 0.05, ** p < 0.01, and *** p < 0.001. Independent t-test was used for statistical analysis. The values are shown as mean with a 95% confidence interval (CI).
Fig 3: The effects of IL3RA knockdown on mRNA expression of estrogen receptors (EsRa and EsRb), MMPs, and COL11A2 in chondrocytes from human females. The fold change in gene expression in each knockdown (KD) group compared with that in the non-KD control was determined using qPCR. For each group, the assay was performed in triplicate, and chondrocytes were treated with 10 nM of IL-3. The effects are presented for different concentrations of estrogen or testosterone. n = 3 each group. * p < 0.05, ** p < 0.01. Independent t-test was used for statistical analysis. The values are shown as mean with a 95% confidence interval (CI).
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