Human / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag) 90182-C08H from Sino Biological, Inc.

Description

A DNA sequence encoding the human/cynomolgus/rhesus CD28 (P10747-1 & Q0PDN3-1 & NP_001036106.2) (Asn19-Pro152) was expressed with a polyhistidine tag at the C-terminus and a signal peptide (Q0PDN3-1/NP_001036106.2, Met1-Gly18) at the N-terminus. Human, Cynomolgus and Rhesus CD28 sequences (Asn19-Pro152) are identical

Product Specs
ItemHuman / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag)
CompanySino Biological, Inc.
Price $218.00Supplier Page View Company Product Page
Catalog Number90182-C08H
Size50 µg
FormatLyophilized from sterile PBS, pH 7.4
Conjugate/TagC-His
Purity≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested)
Activity1.Immobilized Recombinant Human / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag) (Cat: 90182-C08H) at 2 µg/mL (100 µL/well) can bind Recombinant Cynomolgus / Rhesus CD80 / B7-1 Protein (Fc Tag) (Cat: 90268-C02H), the EC50 is 150-600 ng/mL. 2.Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on proA Biosensor, can bind Recombinant Human, Cynomolgus, Rhesus CD28 Protein, His Tag (Cat. No. 90182-C08H) with an affinity constant of 0.895 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
TypeRecombinant protein
Molecule NameCD28
SpeciesHuman, Cynomolgus, Rhesus
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human/cynomolgus/rhesus CD28 consists of 145 amino acids and predicts a molecular mass of 16.6 kDa. The molecular weight of this protein is around 64.5 kDa verified by SEC-MALS (Routinely tested)
NCBI Gene AliasesTp44
NCBI Full Gene NameCD28 molecule