Fig 2: Localization of MALT1 and phos-NF-κB (p65) in different type of cells in the spinal cord of rats. (A) Double immunofluorescent staining showed colocalization of MALT1 or phos-NF-κB (p65) expression in astrocytes (GFAP positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (B, C) The quantified colocalization of MALT1 or phos-NF-κB (p65) with GFAP were assessed using the Pearson coefficient. (D) Double immunofluorescent staining to check colocalization of MALT1 or phos-NF-κB (p65) expression in microglia (Iba-1 positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (E, F) The quantified colocalization of MALT1 or phos-NF-κB (p65) with Iba-1 were assessed using the Pearson coefficient. (G) Double immunofluorescent staining showed MALT1 or phos-NF-κB (p65) expression in neurons (NeuN positive cells) in the anterior horn of spinal cord. Scar bar = 40μm. (H, I) The quantified colocalization of MALT1 or phos-NF-κB (p65) with NeuN were assessed using the Pearson coefficient. (J) Double immunofluorescent staining to colocalize MALT1 or phos-NF-κB (p65) with endothelial cells (CD31 positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (K, L) The quantified colocalization of MALT1 or phos-NF-κB (p65) with CD31 were assessed using the Pearson coefficient. Data are presented as the mean ± SEM. n = 6 per group. *P < 0.05 vs sham group, #P < 0.05 vs SCI/R group.

Fig 3: Effects of MI-2 on the spinal MALT1 expression and phos-NF-κB (p65) activation in SCI/R rats. (A) Immunofluorescent staining of MALT1 in the anterior horn of rat’s spinal cord. Scale bar = 50μm. (B) Immunofluorescent density analysis to quantify the expression of MALT1. (C) Immunofluorescent staining of [phos-NF-κB (p65)]-positivities. Scale bar = 50μm. (D) Immunofluorescent density analysis to quantify the expression of phos-NF-κB (p65). Data are presented as the mean ± SEM. n = 6 per group. *P < 0.05 vs sham group, #P < 0.05 vs SCI/R group.

Fig 4: Localization of MALT1 and phos-NF-κB (p65) in different type of cells in the spinal cord of rats. (A) Double immunofluorescent staining showed colocalization of MALT1 or phos-NF-κB (p65) expression in astrocytes (GFAP positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (B, C) The quantified colocalization of MALT1 or phos-NF-κB (p65) with GFAP were assessed using the Pearson coefficient. (D) Double immunofluorescent staining to check colocalization of MALT1 or phos-NF-κB (p65) expression in microglia (Iba-1 positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (E, F) The quantified colocalization of MALT1 or phos-NF-κB (p65) with Iba-1 were assessed using the Pearson coefficient. (G) Double immunofluorescent staining showed MALT1 or phos-NF-κB (p65) expression in neurons (NeuN positive cells) in the anterior horn of spinal cord. Scar bar = 40μm. (H, I) The quantified colocalization of MALT1 or phos-NF-κB (p65) with NeuN were assessed using the Pearson coefficient. (J) Double immunofluorescent staining to colocalize MALT1 or phos-NF-κB (p65) with endothelial cells (CD31 positive cells) in the anterior horn of spinal cord. Scar bar = 20μm. (K, L) The quantified colocalization of MALT1 or phos-NF-κB (p65) with CD31 were assessed using the Pearson coefficient. Data are presented as the mean ± SEM. n = 6 per group. *P < 0.05 vs sham group, #P < 0.05 vs SCI/R group.