Fig 1: Colocalization of Lin28 with Akt in the VMH neurons of 6-month-old (a–c), 12-month-old (d–f) and 24-month-old (g–i) male rats. Double labeling of Lin28 (a,d,g, green, left panel), Akt (b,e,h, red, middle) and composite image (c,f,i, right). Bar, 25 µm.
Fig 2: Percentage of Lin28-IR neurons, colocalizing AkT, PI3K, mTOR and Raptor in the VMH of rats of different age group. n = 5 per group (* p < 0.05, comparing 2-month-old rats; ● p < 0.05, comparing 12-month-old rats; ♦ p < 0.05, comparing 24-month-old rats).
Fig 3: Immunostaining of Lin28-IR neurons in the VMH of 6-month-old (a–c), 12-month-old (d–f) and 24-month-old (g–i) male rats. Double labeling of neuronal marker NGreen (a,d,g, green, left panel), Lin28 (b,e,h, red, middle) and composite image (c,f,i, right). Bar, 25 µm.
Fig 4: Colocalization of Lin28 with mTOR in the VMH neurons of 6-month-old (a–c), 12-month-old (d–f) and 24-month-old (g–i) male rats. Double labeling of Lin28 (a,d,g, green, left panel), mTOR (b,e,h, red, middle) and composite image (c,f,i, right). Bar, 25 µm.
Fig 5: Colocalization of Lin28 with PI3K in the VMH neurons of 6-month-old (a–c), 12-month-old (d–f) and 24-month-old (g–i) male rats. Double labeling of Lin28 (a,d,g, green, left panel), PI3K (b,e,h, red, middle) and composite image (c,f,i, right). Bar, 25 µm.
Supplier Page from Abcam for Recombinant Human Lin28A protein (DDDDK tag N-Terminus + Avi tag N-Terminus)