Recombinant SARS-CoV-2 Spike His Protein, CF 10549-CV-100 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant SARS-CoV-2 Spike His Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $617.00Supplier Page View Company Product Page
Catalog Number10549-CV-100
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose
ApplicationsBioactivity
Size100 ug (also 1 mg)
Conjugate/TagUnconjugated
Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecule NameSpike
Source/Expression SystemHEK293-derived Recombinant SARS-CoV-2 Spike His Protein, CF
Molecular WeightObserved: 144-175 kDa, under reducing conditions
Theoretical: 134 kDa
SpeciesSARS-CoV-2
NCBI Gene Aliasesspike glycoprotein
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The monomeric but not homotrimeric spike protein of SARS-CoV-2 activates TLR4 signalingMolecular biology reportsJune 23, 2025Lübow C, Weindl G.Wiesbaden, Germany) were used: S1 subunit (10569-CV-100), S2 subunit (10594-CV-100), spike protein (10549-CV-100), spike (GCN4-IZ) protein (10561-CV-100), B.1.1.7 spike (GCN4-IZ) protein (10796-CV-100), and B.1.617.2

(2) Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potencyNature biotechnologyMay 1, 2025McGee JE, Kirsch JR, Kenney D, Cerbo F, Chavez EC, Shih TY, Douam F, Wong WW, Grinstaff MW.

(3) The influence of SARS-CoV-2 spike protein exposure on retinal development in the human retinal organoidsCell BiosciApril 11, 2025Jing Gong, Lingling Ge, Yuxiao Zeng, Cao Yang, Yushan Luo, Jiahui Kang, Ting Zou, Haiwei Xuculture medium to obtain mature retinal organoids. Spike protein exposure The S protein (R&D Systems, 10549-CV-100) was applied in present study according to the manufacturer’s instructions. Similar to the S protein

(4) Potent neutralization by a receptor binding domain monoclonal antibody with broad specificity for SARS-CoV-2 JN.1 and other variantsbioRxiv : the preprint server for biologyApril 29, 2024Piepenbrink MS, Khalil AM, Chang A, Mostafa A, Basu M, Sarkar S, Panjwani S, Ha YH, Ma Y, Ye C, et al.

(5) Human surfactant protein A inhibits SARS-CoV-2 infectivity and alleviates lung injury in a mouse infection modelFrontiers in ImmunologyMarch 26, 2024Ikechukwu B. Jacob, Amanda Gemmiti, Weichuan Xiong, Erin Reynolds, Brian Nicholas, Saravanan Thangamani, Hongpeng Jia, Guirong Wangmicrotiter plates were coated with 1 µg/ml recombinant SARS-CoV-2 spike protein produced in HEK293T cells (10549-CV-100, R&D Systems and Biotech, NE, MN, USA) while some plates were coated with 50 ng/ml SARS-CoV-2 S protein

(6) Choice of adjuvant and antigen composition alters the immunogenic profile of a SARS-CoV-2 subunit vaccineFrontiers in Drug DeliveryFebruary 7, 2024William R. Lykins, Jeroen Pollet, Jessica A. White, Brian Keegan, Leroy Versteeg, Ulrich Strych, Wen-Hsiang Chen, Raodoh Mohamath, Gabi Ramer-Denisoff, Sierra Reed, et al.recombinant pre-fusion stabilized SARS-CoV-2 full-length wt Spike His Protein (R&D Systems, Minneapolis, MN, #10549-CV-100) in coating buffer (eBioscience #00004459) and incubated at 4°C overnight. Plates were then washed with

(7) Investigation of SARS-CoV-2 individual proteins reveals the in vitro and in vivo immunogenicity of membrane proteinScientific reportsDecember 18, 2023Haystead T, Lee E, Cho K, Gullickson G, Hughes P, Krafsur G, Freeze R, Scarneo S.Spike Protein RBD Subunit,R&D Systems,10500-CV-100 SARS-CoV-2 Spike Protein Full Length,R&D Systems,10549-CV-100 SARS-CoV-2 Nucleocapsid,R&D Systems,10474-CV-050 Reagents.

(8) Class switch towards spike protein-specific IgG4 antibodies after SARS-CoV-2 mRNA vaccination depends on prior infection historyScientific reportsAugust 13, 2023Kiszel P, Sík P, Miklós J, Kajdácsi E, Sinkovits G, Cervenak L, Prohászka Z.

(9) Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primatesMolecular therapy. Nucleic acidsDecember 13, 2022Suzuki Y, Miyazaki T, Muto H, Kubara K, Mukai Y, Watari R, Sato S, Kondo K, Tsukumo SI, Yasutomo K, et al.containing MC3, or ALUM-adjuvanted (Thermo Scientific #77161) SARS-CoV-2 spike protein (S-2P) (R&D Systems #10549-CV-100) with mRNA and protein doses of 0.2–1 μg and 0.2–5 μg, respectively. Mice were euthanized at day 35

(10) Evidence, detailed characterization and clinical context of complement activation in acute multisystem inflammatory syndrome in childrenScientific reportsNovember 17, 2022Sinkovits G, Schnur J, Hurler L, Kiszel P, Prohászka ZZ, Sík P, Kajdácsi E, Cervenak L, Maráczi V, Dávid M, et al.

(11) A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stabilityNPJ vaccinesNovember 2, 2022Voigt EA, Gerhardt A, Hanson D, Jennewein MF, Battisti P, Reed S, Singh J, Mohamath R, Bakken J, Beaver S, et al.plates were coated with 100 µL of Recombinant SARS-CoV-2 Spike His Protein, Carrier Free, (R&D Systems, #10549-CV-100) at a concentration of 2 µg/mL diluted in DPBS (Gibco). Plates were incubated overnight at 4 °C, washed

(12) An experimental test of the nicotinic hypothesis of COVID-19Proceedings of the National Academy of Sciences of the United States of AmericaNovember 1, 2022Godellas NE, Cymes GD, Grosman C.

(13) Calcium dobesilate reduces SARS-CoV-2 entry into endothelial cells by inhibiting virus binding to heparan sulfateScientific reportsOctober 7, 2022Kiyan Y, Schultalbers A, Chernobrivaia E, Tkachuk S, Rong S, Shushakova N, Haller H.

(14) Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensorsScientific reportsSeptember 15, 2022Li H, Zhu B, Li B, Chen L, Ning X, Dong H, Liang J, Yang X, Dong J, Ueda H.on the plate at the concentration of 5 µg/ml spike proteins (Spike protein of wild-type SARS-CoV-2, 10549-CV-100, R&D Systems; Spike protein of SARS-CoV-2 previous variant of concern alpha, 40589-V08B6, Sino Biological;

(15) Waning of SARS-CoV-2 Seropositivity among Healthy Young Adults over Seven MonthsVaccinesSeptember 15, 2022Lea CS, Simeonsson K, Kipp AM, McNeill C, Wilcox L, Irish W, Morris H, Diaz OM, Fallon JT, Roper RL.

(16) SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy PathogenesisInternational journal of molecular sciencesSeptember 9, 2022Bhargavan B, Kanmogne GD.thiol-based drugs. 4. Materials and Methods 4.1. Reagents Recombinant SARS-CoV-2 S-proteins, SW (cat. #10549-CV-100), SD (cat. #10878-CV-100), rhACE2 (cat. #933-ZN-010), rTFPI (cat. #2974-PI-010), and TM (BDCA3, cat.

(17) Potent universal beta-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibodyPLoS pathogensJuly 1, 2022Piepenbrink MS, Park JG, Deshpande A, Loos A, Ye C, Basu M, Sarkar S, Khalil AM, Chauvin D, Woo J, et al.

(18) Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccineJournal of controlled release : official journal of the Controlled Release SocietyJuly 1, 2022Atalis A, Keenum MC, Pandey B, Beach A, Pradhan P, Vantucci C, O'Farrell L, Noel R, Jain R, Hosten J, et al.mice received 1 μg of unformulated (i.e., soluble) stabilized SARS-CoV-2 spike protein (R&D Systems, #10549-CV-100) and 4 mg adjuvant-NPs with total adjuvant doses of MPLA (24 μg), CpG (20 μg), PUUC (17 μg), CpG+PUUC

(19) Cross-reactive antibodies elicited to conserved epitopes on SARS-CoV-2 spike protein after infection and vaccinationScientific reportsApril 20, 2022Geanes ES, LeMaster C, Fraley ER, Khanal S, McLennan R, Grundberg E, Selvarangan R, Bradley T.

(20) The spike protein of SARS-CoV-2 induces endothelial inflammation through integrin α5β1 and NF-κB signalingThe Journal of biological chemistryMarch 1, 2022Robles JP, Zamora M, Adan-Castro E, Siqueiros-Marquez L, Martinez de la Escalera G, Clapp C.

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(1)

7/6/2022Spike Protein Effectively Stimulates Immune Cells Scott ScarneoHere, we stimulated human macrophages with this protein to measure downstream inflammatory markers. The protein was pure with no evidence of LPS.

Spike His-tag Protein

Spike His-tag Protein

Spike His-tag Protein

Spike His-tag Protein

Spike His-tag Protein

Recombinant SARS-CoV-2 Spike His Protein, CF from R&D Systems, a Bio-Techne Brand

Description

R&D Systems, a Bio-Techne Brand

Citations (24)

Reviews

Recombinant SARS-CoV-2 Spike His Protein, CF from R&D Systems, a Bio-Techne Brand

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