UltraScript® cDNA Synthesis Kit PB30.11-02 from PCR Biosystems

Product Specs
ItemUltraScript® cDNA Synthesis Kit
CompanyPCR Biosystems
Price $157.00Supplier Page View Company Product Page
Catalog NumberPB30.11-02
Size25 reactions
ApplicationscDNA Synthesis for qPCR Analysis, cDNA synthesis from low copy transcripts, Viral RNA Targets
TypeEnzyme
Research AreaGenetics, Microbiology, Molecular Biology, Ecology, Biomedical Science, Virology, Immunology

Specifications/Features

Features:

Thermostable reverse transcriptase 45°C to 55°C
Advanced RNase inhibitor
High cDNA yields from as little as 4pg total RNA
Accurate reverse transcription of GC-rich templates
Sensitive detection of low copy number transcripts
Advanced buffer chemistry including Mg and dNTPs
Both premixed or separate oligo cDNA synthesis kits available

Add to Compare List

PCR Biosystems

Aztec House

397-405 Archway Road

London N6 43R

United Kingdom

Phone: + 44 (0) 20 3936 8101

Email: info@pcrbio.com

Company Profile

Website: www.pcrbio.com

(1) Sex-specific division of labor in termites is linked to differential brain architecture and foraging gene expressioniScienceApril 17, 2026Iago Bueno da Silva, Arthur Roque Justino, Jaqueline Eterna Batista, Lohan Valadares, Klaus Hartfelder, Fabio Santos do NascimentoIllustra GFX PCR DNA and Gel Band Purification Kit,Cytiva,Cat#28903470 Ultrascript cDNA Synthesis Kit,PCR Biosystems,PB30.11–10 qPCRBIO SyGreen Mix Separate-Rox,PCR Biosystems,PB20.14–05 ,, Deposited data,Deposited data,Deposited

(2) Cisplatin Induces Inflammation by Activating IL-6 via Suppressing rno-let-7g-5p and rno-let-7f-5p Expression in Intestinal Epithelial CellsInternational Journal of Medical SciencesMarch 17, 2026Chi-Jen Chang, Tsung-Ming Chang, Ying-Sui Sun, Kuan-Ting Lu, Ju-Fang LiuFirst-strand cDNA synthesis for mRNA analysis was conducted using the qPCRBIO cDNA Synthesis Kit (Cat. #PB30.11-10, PCR Biosystems, UK), and miRNA cDNA synthesis was performed using the Mir-X miRNA First-Strand Synthesis

(3) An innovative in vitro model for studying the biology of cardiac fibroblasts originating from the epicardiumDis Model MechMarch 1, 2026Claudia Müller-Sánchez, María Gertrudis Muñiz-Banciella, Manuel Reina, Francesc X. Soriano, Ofelia M. Martínez-Estradapurified RNA was reverse transcribed into cDNA using UltraScript® Reverse Transcriptase (PCR Biosystems, PB30.11-10). Relative gene expression levels were quantified using SYBR Master Mix (Promega, A6002); real-time PCR

(4) Formation of a membraneless compartment regulates bacterial virulenceNat CommunApril 23, 2025Lior Aroeti, Netanel Elbaz, Raya Faigenbaum-Romm, Oren Yakovian, Yael Altuvia, Liron Argaman, Naama Katsowich, Michal Bejerano-Sagie, Miriam Ravins, Hanah Margalit, et al.using primers #1952 and #1953. cDNA was synthesized using qPCRBIO high-quality cDNA synthesis kit (PB30.11-10, PCR Biosystems) and quantified by Real-Time PCR (RT-PCR) using iTaq Universal SYBR Green Supermix (Cat.

(5) Dysregulation of Leukaemia Inhibitory Factor (LIF) Signalling Pathway by Supraphysiological Dose of Testosterone in Female Sprague Dawley Rats During Development of Endometrial ReceptivityBiomedicinesJanuary 24, 2025Allia Najmie Muhammad Yusuf, Mohd Fariz Amri, Azizah Ugusman, Adila A Hamid, Izzat Zulhilmi Abd Rahman, Mohd Helmy Mokhtar400 ng of RNA was converted to complementary DNA (cDNA) utilising the qPCRBio cDNA synthesis kit (CAT PB30.11-10, PCR Biosystems, London, UK). As a control, this material was amplified without the addition of reverse

(6) Optimizing approaches for targeted integration of transgenic cassettes by integrase-mediated cassette exchange in mouse and human stem cellsStem CellsJanuary 17, 2025Phalguni Rath, Philipp Kramer, Daniel Biggs, Chris Preece, Nicole Hortin, Rebeca Diaz, Marta Perez-Alcantara, Xiang Li, Arnaud Bolard, Nicola Beer, et al.#15596026), and 400 ng of RNA was reverse transcribed using the qPCRBIO cDNA synthesis kit (PCR Biosystems, #PB30.11-02) according to manufacturer’s instructions. Quantitative PCR was carried out using PowerUp SYBR Green Master Mix

(7) Supraphysiological Dose of Testosterone Impairs the Expression and Distribution of Sex Steroid Receptors during Endometrial Receptivity Development in Female Sprague–Dawley RatsInternational Journal of Molecular SciencesSeptember 23, 2024Allia Najmie Muhammad Yusuf, Mohd Fariz Amri, Azizah Ugusman, Adila A Hamid, Mohd Helmy MokhtarConversion of RNA to complementary DNA (cDNA) was performed using the qPCRBio cDNA synthesis kit (CAT PB30.11-10 PCR Biosystems, London, UK). Amplification of samples without the addition of reverse transcriptase

(8) Proteasome gene expression is controlled by coordinated functions of multiple transcription factorsJournal of Cell BiologyAugust 5, 2024Jennifer E. Gilda, Asrafun Nahar, Dharanibalan Kasiviswanathan, Nadav Tropp, Tamar Gilinski, Tamar Lahav, Dina Alexandrovich, Yael Mandel-Gutfreund, Soyeon Park, Shenhav ShemerSigma-Aldrich) and served as a platform for cDNA synthesis using qPCRBIO cDNA Synthesis Kit (Cat. #PB30.11-10; PCR Biosystems). Real-time PCR was performed with primers to mouse genes (Table S2) using PerfeCTa

(9) A transcriptomics-based drug repositioning approach to identify drugs with similar activities for the treatment of muscle pathologies in spinal muscular atrophy (SMA) modelsHuman Molecular GeneticsFebruary 18, 2024Joseph M Hoolachan, Eve McCallion, Emma R Sutton, Özge Çetin, Paloma Pacheco-Torres, Maria Dimitriadi, Suat Sari, Gavin J Miller, Magnus Okoh, Lisa M Walter, et al.(ThermoScientific) before reverse transcription was performed using the qPCRBIO cDNA Synthesis Kit (#PB30.11-10, PCR Biosystems) as per manufacturer’s instructions. The cDNA was then diluted by 1:5 in nuclease-free

(10) Cholesterol modulates type I/II TGF-β receptor complexes and alters the balance between Smad and Akt signaling in hepatocytesCommunications BiologyJanuary 2, 2024Roohi Chaudhary, Laureen S. Goodman, Sai Wang, Anastasia Asimakopoulos, Ralf Weiskirchen, Steven Dooley, Marcelo Ehrlich, Yoav I. Henismanufacturer’s instructions. RNA was reverse transcribed to cDNA using qPCRBIO cDNA Synthesis kit (cat. #PB30.11, PCR Biosystems, London, UK). The mRNA levels of endogenous TGFBR1 were determined in triplicate by

(11) Abnormal adipogenic signaling in the bone marrow mesenchymal stem cells contributes to supportive microenvironment for leukemia developmentCell communication and signaling : CCSOctober 10, 2023Sabbah R, Saadi S, Shahar-Gabay T, Gerassy S, Yehudai-Resheff S, Zuckerman T.cat. #35,300). cDNA was synthesized using the qPCRBIO cDNA Synthesis kit (PCR Biosystems Ltd, cat. #PB30.11–10, London, UK). qPCR was performed using Absolute Blue qPCR SYBR Green ROX Mix (Thermo Fisher Scientific,

(12) A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of GenesBio ProtocAugust 5, 2023William Bezodis, Helen Prescott, Daniela Vlad, Hugh Dickinson(Thermo Fisher Scientific, catalog number: ER1011)qPCRBIO cDNA synthesis kit (PCR Biosystems Ltd, catalog number: PB30.11-10)SuperScriptTM IV First-Strand Synthesis System (Thermo Fisher Scientific, catalog number: 18091050)qPCR

(13) Molecular components of the circadian clock regulate HIV-1 replicationiScienceJuly 21, 2023Helene Borrmann, Görkem Ulkar, Anna E. Kliszczak, Dini Ismed, Mirjam Schilling, Andrea Magri, James M. Harris, Peter Balfe, Sridhar Vasudevan, Persephone Borrow, et al.assays ,, CytoTox 96 Non-Radioactive Cytotoxicity Assay,Promega,G1780 cDNA Synthesis Kit,PCR Biosystems Ltd,PB30.11-10 qPCRBIO SyGreen Blue Mix,PCR Biosystems Ltd,PB20.17-20 Firefly Luciferase Assay kit,Promega,E1501 ,, Experimental models

(14) CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathwayInternational journal of biological sciencesJanuary 1, 2023Chang TM, Chiang YC, Lee CW, Lin CM, Fang ML, Chi MC, Liu JF, Kou YR.Seongnam-si, Gyeonggi-do, KOR). First-stand cDNA was obtained using a qPCRBIO cDNA Synthesis Kit (Cat. No. PB30.11-10, PCR biosystems, London, England, UK). Human CXCL14, CDH1, CDH2, VIM, SNAI1, and GAPDH mRNA expression

(15) Androgen-Independent Prostate Cancer Is Sensitive to CDC42-PAK7 Kinase InhibitionBiomedicinesDecember 30, 2022Hyunho Han, Cheol Keun Park, Young-Deuk Choi, Nam Hoon Cho, Jongsoo Lee, Kang Su ChoRT-PCR blot, we first performed reverse transcription on total RNA samples using the cDNA synthesis kit (PB30.11 PCR Biosystems Ltd., Plymouth, PA, USA). The cDNA samples were then amplified using specific primers

(16) Phytocannabinoids Act Synergistically with Non-Steroidal Anti-Inflammatory Drugs Reducing Inflammation in 2D and 3D In Vitro ModelsPharmaceuticals (Basel)December 14, 2022Ajjampura C. Vinayaka, Nurit Shalev, Seegehalli M. Anil, Sudeep Tiwari, Navin Kumar, Eduard Belausov, Karthik Ananth Mani, Guy Mechrez, Hinanit KoltaiThermo Scientific, Waltham, MA, USA). RNA was reverse-transcribed using qPCRBIO cDNA Synthesis Kit (PB30.11-10; PCR Biosystems IncqPCRBIO, Wayne, PA, USA). Primers were designed and PCR was performed using qPCRBIO SyGreen Blue Mix Hi-ROX

(17) Transcriptome Analysis Reveals Vimentin-Induced Disruption of Cell-Cell Associations Augments Breast Cancer Cell MigrationCellsDecember 13, 2022Usman S, Jamal A, Bushaala A, Waseem NH, Al-Dehlawi H, Yeudall WA, Teh MT, Tummala H, Waseem A.the cDNA with a mixture of oligo-dT and random hexamers using the qPCRBIO cDNA Synthesis Kit (Cat # PB30.11-10, PCR Biosystems, London, UK) in triplicates according to the manufacturer’s instructions. For total

(18) Effect of hydroalcoholic seed extract of Nigella sativa on hepatic and pancreatic factors of Nrf2 and FGF21 in the regulation of insulin transcription factors of MafA and PDX-1 in streptozotocin-treated diabetic ratsNutrition & metabolismSeptember 15, 2022Soleimani-Dodran M, Alipanah-Moghadam R, Jeddi F, Babaei M, Salimnejad R, Bahreini E.Hamburg, Germany). Total RNA was reverse transcribed into cDNA using the qPCRBIO cDNA synthesis kit (PB30.11-10, PCR Biosystems, UK) according to the manufacturer's instructions. The GAPDH gene (glyceraldehyde-3-phosphate

(19) A mind in motion: Exercise improves cognitive flexibility, impulsivity and alters dopamine receptor gene expression in a Parkinsonian rat modelCurrent Research in NeurobiologyMay 1, 2022Wang Zhuo, Adam J. Lundquist, Erin K. Donahue, Yumei Guo, Derek Phillips, Giselle M. Petzinger, Michael W. Jakowec, Daniel P. HolschneiderComplementary DNA (cDNA) was synthesized from 1 μg isolated RNA using the qPCRBIO cDNA Synthesis Kit (Cat. No. PB30.11–10, PCR Biosystems, Wayne, PA, USA) following manufacturer's guidelines before being diluted 1:5 in DNAse/RNAse

(20) Specific Compositions of Cannabis sativa Compounds Have Cytotoxic Activity and Inhibit Motility and Colony Formation of Human Glioblastoma Cells In VitroCancersApril 5, 2021Peeri H, Shalev N, Vinayaka AC, Nizar R, Kazimirsky G, Namdar D, Anil SM, Belausov E, Brodie C, Koltai H.(T9424; Sigma Aldrich, St. Louis, MO, USA). RNA was reverse-transcribed in a total volume of 20 μL (PB30.11-10; PCR Biosystems IncqPCRBIO, Wayne, PA, USA) according to the manufacturer’s protocol. PCR was performed

Be the First to Write a Review!

UltraScript® cDNA Synthesis Kit

qPCRBIO cDNA Synthesis Kit

UltraScript® cDNA Synthesis Kit from PCR Biosystems

Description

Specifications/Features

PCR Biosystems

Citations (30)

Reviews

UltraScript® cDNA Synthesis Kit from PCR Biosystems

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map