Fig 1: Acylation-deficient mutant of ZAP-70 is catalytically active.A, Schematic representation of ZAP-70 showing the position of the S-acylated cysteine residue (C564) in the kinase domain. B, In vitro kinase assay performed using ZAP-70 immunoprecipitated from P116 (ZAP-70 null) Jurkat T cells expressing WT or acylation-deficient C564R ZAP-70. Phosphorylation of SLP-76 was detected using phospho-SLP-76 (Y128) antibody. C, Quantified data showing phosphorylation of SLP-76 by WT or C564R ZAP-70. Data shown are representative of three independent biological repeats and represented as mean ± SEM, normalized to total SLP-76.