Three independent electrophoretic mobility shift assay experiments indicate no specific binding of CTCF at MAP2K5 cis-eQTL SNP rs4776984. Biotinylated (labeled probe) 31-bp oligonucleotide complexes with +/-15 bp flanking the reference or alternate allele for variant rs4776984 were incubated with CTCF protein (OriGene TP720882) and/or human white adipocyte (HWA) nuclear protein and resolved on a 6% polyacrylamide gel. Competitor assays were performed by incubating the reaction with 100X excess of unlabeled (no biotin) oligonucleotide complexes with the identical sequences. Figure cited from Nat Commun, PMID: 29666371
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