Fig 1: P. anaerobius attenuated the therapeutic effect of oxaliplatin in CRC mice. (A) Representative morphologies and histological images (H&E) of CRC tissues. Scale bar: 100 µm (left), 25µm (right). (B) Schematic diagram of experimental design and timeline of CRC mice model (n = 6). PA: P. anaerobius; OXA: oxaliplatin; anti-Gr-1mab: anti-mouse Gr-1 monoclonal antibody; anti-IL-23 mAb: anti-mouse IL-23 monoclonal antibody. (C) The amount of P. anaerobius in stool samples of CRC mice determined by qPCR (mean ± SD, two-tailed unpaired Student’s t-test). (D) Representative TEM images of P. anaerobius (red arrows) attaching to colon tumor tissue. Scale bars: 500 nm for PA images, 2 μm for colon tumor tissue images. (E) Representative tumor images and statistical analysis of tumor weights of CRC in different groups (n = 6, mean ± SD, one-way analysis of variance).
Fig 2: IL-23 activated the EMT signaling pathway. (A) The morphology of MC-38 cells after incubation (48 h) with MDSCs-CM, IL-23, anti-IL-23+MDSCs-CM, respectively. (B) Representative images of wound healing assay of MC-38 cells. (C) Representative images of invasive MC-38 cells. (D) The expression of N-cadherin, E-cadherin, Snail, Slug, Twist, Stat3, and P-stat3 in MC-38 cells detected by Western blot. (B–D) Data were presented as the mean ± SD; p-values were determined by one-way analysis of variance. Three independent experiments were performed with consistent results.
Fig 3: IL-23 released by MDSCs promoted chemoresistance of CRC. (A) The expression levels of IL-6, VEGF, HGF, and IL-23 in culture medium of MDSCs determined by ELISA. (B) IL-23 levels in tumor tissues with different treatments. (C) The viability of MC-38 cells treated with different concentrations of oxaliplatin (CCK-8 assay). (D) The viability of MC-38 cells treated with oxaliplatin after pretreatment with MDSCs-CM, recombinant IL-23, or anti-IL-23+MDSCs-CM, respectively. (E) The expression of chemoresistance biomarkers of ABCB1, ABCC1, and ERCC1 determined by Western blotting (mean ± SD, one-way analysis of variance, triplicated).
Supplier Page from Abcam for Recombinant mouse IL-23 protein (Active)