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HRP and AP Conjugates
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HRP and AP Conjugates
HRP and AP Conjugates from Bio-Rad
(1)
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Citations:
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Figures:
(2)
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Supplier Page from
Bio-Rad for
HRP and AP Conjugates
Description
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
HRP and AP Conjugates
Company
Bio-Rad
Price
Supplier Page
Catalog Number
1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
Applications
Western Blotting
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Bio-Rad
Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive
Hercules
,
California
94547
United States
Phone
: 800-424 6723
800-879 2289
Company Profile
Website:
http://discover.bio-rad.com
Citations (1950)
(1)
Genetic variants of the transporter SLC22A4 affect the abundance and survival of Fusobacterium nucleatum in colorectal cancer
Gut Microbes
December 31, 2026
Samah Chouaibi, Veronica Fertitta, Silvia Porreca, Leila Njim, Antonella Carcagnì, Gabriele Toietta, Gianluca Canettieri, Khadija Zouari, Maha Mastouri, Yosr Kadri,
et al.
anti-mouse IgG (H + L) and
goat anti-rabbit IgG (H + L
) antisera were obtained from
Bio-Rad
(cat. #
1706516
and #
1706515
, respectively). Cell
Infection
in vitro
and Gentamicin protection
assay
Spheroids were
(2)
ER proteostasis failure in HYOU1 deficiency alters B cells, neutrophils, and interferon signalling
J Hum Immun
November 2, 2026
Aida Idani, Sabrina Bibi-Triki, Tristan Stemmelen, Mirjana Radosavljevic, Pierre Grenot, Aurélie Hirschler, Alice Bernard, Michelle Rosenzwajg, Jianying Yang, Alice Eischen,
et al.
conjugates;
1721019
;
Bio-Rad Laboratories
; RRID:AB_11125143, and
Goat Anti-Mouse IgG [H+L]-HRP
conjugates;
1706516
;,
Bio-Rad Laboratories
; RRID:AB_2921252) at a dilution of
1:10,000
for 1 h. The ChemiDoc XRS+ system
(3)
Targeting biglycan-TLR2/TLR4 interaction with synthetic peptides mitigates inflammation and ameliorates renal function in ischemia-reperfusion injury
Cell Mol Biol Lett
September 25, 2026
Rosetta Merline, Louise Tzung-Harn Hsieh, Jinyang Zeng-Brouwers, Rajkumar Vutukuri, David C. Briggs, Anthony J. Day, Malgorzata Wygrecka, Szymon Nowak, Rafal Bartoszewski, Liliana Schaefer
secondary antibody used was
goat
anti-rabbit immunoglobulin G (IgG) (H + L)-HRP conjugate (Cat. no.
1706515
,
1:10,000
;
Bio-Rad Laboratories
GmbH, Feldkirchen, Germany). RNA isolation and
quantitative real-time polymerase chain reaction
(4)
Microbial GAIN domains undergo autoproteolysis and enable release of diverse cell-surface-associated proteins
Current Biology
September 21, 2026
Anna P. Brogan, David Z. Rudner
nanobody,Nanotag,Cat # N1583 anti-ALFA-Alexa647 nanobody,Nanotag,Cat #N1502-AF647-L HRP-anti-rabbit IgG,
BioRad
,Cat #
1706515
HRP-anti-mouse IgG,
BioRad
,Cat #
1706516
Bacterial and
virus
strains
,Bacterial and
virus
strains
,Bacterial
(5)
The p53-Associated Regulation of ATF4 mRNA Expression Involves p21 and p130 in a Stress-Dependent Manner
Cells
September 20, 2026
Alexandra Dalina, Maria Shilyaeva, Irina Kovaleva, Peter Chumakov
Then,
membranes
were washed with PBST and incubated with either anti-rabbit (Bio-Rad Laboratorie; cat#
1706515
) or anti-
mouse
(
Bio-Rad Laboratories
; cat#
1706516
) HRP-conjugated secondary antibodies for 1 h at room
(6)
TDP-43 dysfunction induces cryptic circular RNAs in ALS/FTD
bioRxiv
September 20, 2026
Dario Dattilo, Flaminia Pellegrini, Simone Barattucci, Anna-Leigh Brown, Jose Norberto S. Vargas, Ariana Gatt, Ryan Morrie, Georgiana Miller, Iris Bachmutsky, Zachary McEachin,
et al.
peroxidase (HRP)-conjugated secondary antibodies diluted in 5% milk in TBST (anti-mouse HRP (
Bio-Rad
,
1706516
)
1:10,000
and anti-rabbit HRP (
Bio-Rad
,
1706515
)
1:10,000
), the
membrane
was developed using Immobilon
(7)
Optimizing plasma-derived small extracellular vesicle purification to enhance downstream applications
iScience
September 18, 2026
Fanxiu Ma, Meng Yuan, Yue Zhao, Xiumei Jin, Chengsi Wu, Xin Fang, Zhaodi Jiang, Shuying Liu, Wen-Quan Zou, Zhen Li,
et al.
subsequently incubated with
horseradish
peroxidase-conjugated secondary antibodies (
1:5,000
) (
Biorad
#
1706515
,
1706516
), and the immunoreactive proteins were detected using an ECL kit (Tannon #180–501). Afterward,
(8)
Angiopoietin-Like 4 Induces Upregulation of VEGF and sVCAM1 Through JNK/c-Jun and JAK2/STAT5 Signaling Pathways
Invest Ophthalmol Vis Sci
September 17, 2026
Derek L. Osorio, Ajay Kumar, Alexander Choi, Isabella Sodhi, Chuanyu Guo, Silvia Montaner
Santa Cruz Biotechnology
(Dallas, TX, USA); Tie2Y992 (
AF2720
) from
R&D Systems
; and anti-rabbit HRP (
1706515
) and anti-mouse HRP (
1706516
) secondary antibodies from
Bio-Rad Laboratories
(Hercules, CA, USA). Densitometric
(9)
Honokiol Suppresses Stemness and Sensitizes Triple-Negative Breast Cancer to Chemotherapy via YAP/TAZ-TEAD Inhibition
Oncology Research
September 14, 2026
Jiang-Nan Xia, Shan-Dong Zhu, Wei-Ling Qu, Yi-Lin Hu, Wen-Yi Ma, Wenyan Wang, Qian-Lan Huang, Bing-Yuan Lin, Jia-En Guo, Ying-Wei Li
5% BSA and incubated overnight at 4°C. Secondary antibodies consisted of HRP-conjugated anti-
mouse
(#
1706516
) and HRP-conjugated anti-rabbit (#
1706515
), both from
Bio-Rad
(Hercules, CA, USA). We detected protein
(10)
Targeting the DNA-repair chromatin-modifier protein SMYD3 as a novel epigenetics-based therapy for gastric cancer
J Exp Clin Cancer Res
September 12, 2026
Katia De Marco, Marialaura Latrofa, Giovanna Forte, Martina Lepore Signorile, Elisabetta Di Nicola, Paola Sanese, Candida Fasano, Vittoria Disciglio, Erica Candela, Sergio Coletta,
et al.
Cell Signaling Technology
), and anti-
SMYD3
(
12859
, Cell Signaling Technology). Rabbit IgG HRP and Mouse IgG HRP (
1706515
and
1706516
, respectively,
Bio-Rad Laboratories
) were used as secondary antibodies and were revealed
(11)
Small molecule inhibition of the mitochondrial lipid transfer protein STARD7 attenuates influenza viral replication
PLoS Pathog
September 11, 2026
Shipra Sharma, Oyahida Khatun, Jihaan Adonis, Shaochen You, Kevin Hartenbower, Vedita Anand Singh, Namir Shaabani, Saikat De, Emi R. Matsuo, Rachel Y. Sattler,
et al.
STARD7
(
PA5–112822
,
Thermo Fisher
), FLAG (
F3165
,
Sigma
),
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
(
1706515
,
BIO-RAD
),
Goat Anti-Mouse IgG (H + L)-HRP Conjugate
(
1706516
,
BIO-RAD
), IRDye 680RD Donkey anti-Mouse
(12)
Lean adipocyte oxylipin signaling restrains breast cancer through ferroptosis
Science
September 10, 2026
Meghan C. Curtin, Abigail E. Jackson, Mark D. Lee, Elisabeth A. Brown, J. Alan Maschek, David H. Lum, James E. Cox, Alana L. Welm, Keren I. Hilgendorf
TBST buffer for 10 min and incubated with
Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate
(
Bio-rad
,
1705046
) or
Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate
(
Bio-rad
,
1705047
) in 1% BSA, TBST for 1 h at room
(13)
Therapeutic rescue of pathogenic asparaginyl-tRNA synthetase alleles
Molecular Therapy Nucleic Acids
September 8, 2026
Tristan N. Samuels, Jenica H. Kakadia, Cian Ward, Fatema Abdullatif, Marisa I. Mendes, Desiree E. Smith, Rachel Heilmann, Fanqi Wu, Jakub M. Swiercz, Gajja S. Salamons,
et al.
Secondary antibodies IRDye 800CW
Donkey anti-rabbit IgG
,
goat anti-mouse IgG (H + L)-HRP conjugate
(#
1706516
,
Bio-Rad
), and 600CW
goat
anti-
mouse
(LI-COR) were used at
1:5,000
and
1:10,000
dilutions, respectively.
(14)
Nanoscale junctional membrane curvatures recruit BIN1 and SNX9 for endothelial collective migration
J Cell Biol
September 7, 2026
Vera Janssen, Hannah de Kraker, Jesse S. Aaron, Annett de Haan, Iris de Heer, Satya Khuon, Jason Da Silva, Amber J.M. Driessen, Teng-Leong Chew, Josephine M.E. Tan,
et al.
For western blotting
, secondary anti-
mouse
and rabbit antibodies coupled to HRP were obtained from
Bio-Rad
(Cat #
1706515
, #
1721011
,
1:1,000
for WB
).
Western blots
were imaged using enhanced chemiluminescence detection (#34580;
(15)
Cryo-EM structures of the SurA-BAM complex reveal conformational changes in outer membrane protein assembly
Nat Commun
September 4, 2026
Ryoji Miyazaki, Hidetaka Kohga, Nami Matsuoka, Yuki Maruno, Wataru Yoshimoto, Yutaro S. Takahashi, Dede Heri Yuli Yanto, Yudhi Nugraha, Hideki Shigematsu, Takuya Shiota,
et al.
(HRP)-conjugated secondary antibody (
goat anti-rabbit IgG (H + L)-HRP conjugate
;
Bio-Rad
, catalog no.
1706515
; 1/5000) in PBST. After washing with PBST, the proteins were visualized using Chemi-Lumi One (Nacalai
(16)
Pseudomonas aeruginosa Impairs Mitochondrial Function During Infection of Corneal Epithelial Cells
Invest Ophthalmol Vis Sci
September 3, 2026
Rajalakshmy Ayilam Ramachandran, Joelle T. Abdallah, Abigail M. Blanton, Mahad Rehman, Hamid Baniasadi, Danielle M. Robertson
tag,
membranes
were incubated in an appropriate secondary antibody: anti-rabbit IgG-HRP conjugate (#
1706515
) and anti-mouse IgG (H + L)–HRP conjugate (#
1706516
) (
Bio-Rad
). Image Quant TL Toolbox v8.1 software
(17)
Herpes simplex virus 1 subverts the mitochondrial network to support the infection: A lesson on mitochondrial versatility
bioRxiv
September 2, 2026
Rabina Saud, Kimberly Foster-Lemieur, Brett Duguay, Russell Swerdlow, Maria Kalamvoki
Weller (63–65),N/A
Mouse
-HRP,
Sigma-Aldrich
,
A4416
Rabbit-HRP,
Sigma-Aldrich
,
A0545
Mouse
-AP,
Bio-Rad
,
1706520
Rabbit-AP,
Bio-Rad
,
1706518
Alexa405-α-
mouse
,
Invitrogen
,
A31553
Alexa488-α-
mouse
,
Invitrogen
,
A11001
Alexa595-α-
mouse
,
Invitrogen
,
A11005
Alexa680-α-
mouse
,
Invitrogen
,
A21057
Alexa405-
(18)
STX1B variant-specific synaptic dysfunction is associated with network hyperexcitability in human iPSC-derived neurons
eBioMedicine
September 1, 2026
Carolin Haag, Felix Gsell, Oleg Vinogradov, Morgana Barroso Oquendo, Yuanyuan Liu, Betül Uysal, Heidi Löffler, Fabienne Stehle, Fabian Klopfer, Jan Crönlein,
et al.
incubated for 1 h at room temperature with HRP-conjugated secondary antibodies:
goat
anti-rabbit (
Bio-Rad
1706515
, RRID:AB_3752686),
goat
anti-
chicken
(Promega G135A, RRID:AB_430845), or
goat
anti-
mouse
(
Bio-Rad
172-1011
,
(19)
Leveraging the dominant-negative effect of the kuru-protective G127V prion protein variant as a novel therapeutic strategy
Neurobiology of Disease
September 1, 2026
Jean R.P. Gatdula, Nadia A. Mirza-Romero, Isabel C. Orbe, Linnea M. Saunders, Samantha G. Tolton, Janelle S. Vultaggio, Robert C.C. Mercer, Jason C. Bartz, Glenn C. Telling, Hasier Eraña,
et al.
A2228
). The secondary antibodies used were
goat anti-human IgG (H + L) HRP
conjugate (
1:10000
;
BioRad
,
1721050
) and
goat anti-mouse IgG (H + L) HRP conjugate
(
1:10000
;
BioRad
,
1706516
). 2.11. PIPLC treatment of
(20)
Differential toll-like receptor signaling pathways shape IL-1β-dependent inflammatory responses in chicken macrophages
Poultry Science
September 1, 2026
Seungyoon Roh, Tina Sørensen Dalgaard, Ngo Thuy Bao Tran, Tae Sub Park, Cheol-Heui Yun
(HRP)-conjugated secondary antibodies for one hour at room temperature.
Goat anti-mouse IgG-HRP
(
Bio-Rad
,
1706516
) and
goat anti-rabbit IgG (H+L)-HRP
(
Invitrogen
,
31460
) were used as secondary antibodies. After five
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Reviews
Write a Review
(1)
9/13/2022
Reliable Secondary Antibody with Clear Detection
Arianna Napoli
I used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ...
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HRP and AP Conjugates from Bio-Rad
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