HRP and AP Conjugates 1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403 from Bio-Rad

Product Specs
ItemHRP and AP Conjugates
CompanyBio-Rad
Price Supplier Page
Catalog Number1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
ApplicationsWestern Blotting

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

(1) Genetic variants of the transporter SLC22A4 affect the abundance and survival of Fusobacterium nucleatum in colorectal cancerGut MicrobesDecember 31, 2026Samah Chouaibi, Veronica Fertitta, Silvia Porreca, Leila Njim, Antonella Carcagnì, Gabriele Toietta, Gianluca Canettieri, Khadija Zouari, Maha Mastouri, Yosr Kadri, et al.anti-mouse IgG (H + L) and goat anti-rabbit IgG (H + L) antisera were obtained from Bio-Rad (cat. #1706516 and #1706515, respectively). Cell Infection in vitro and Gentamicin protection assay Spheroids were

(2) Therapeutic rescue of pathogenic asparaginyl-tRNA synthetase allelesMolecular Therapy Nucleic AcidsSeptember 8, 2026Tristan N. Samuels, Jenica H. Kakadia, Cian Ward, Fatema Abdullatif, Marisa I. Mendes, Desiree E. Smith, Rachel Heilmann, Fanqi Wu, Jakub M. Swiercz, Gajja S. Salamons, et al.Secondary antibodies IRDye 800CW Donkey anti-rabbit IgG, goat anti-mouse IgG (H + L)-HRP conjugate (#1706516, Bio-Rad), and 600CW goat anti-mouse (LI-COR) were used at 1:5,000 and 1:10,000 dilutions, respectively.

(3) Nanoscale junctional membrane curvatures recruit BIN1 and SNX9 for endothelial collective migrationJ Cell BiolSeptember 7, 2026Vera Janssen, Hannah de Kraker, Jesse S. Aaron, Annett de Haan, Iris de Heer, Satya Khuon, Jason Da Silva, Amber J.M. Driessen, Teng-Leong Chew, Josephine M.E. Tan, et al.For western blotting, secondary anti-mouse and rabbit antibodies coupled to HRP were obtained from Bio-Rad (Cat #1706515, #1721011, 1:1,000 for WB). Western blots were imaged using enhanced chemiluminescence detection (#34580;

(4) Differential toll-like receptor signaling pathways shape IL-1β-dependent inflammatory responses in chicken macrophagesPoultry ScienceSeptember 1, 2026Seungyoon Roh, Tina Sørensen Dalgaard, Ngo Thuy Bao Tran, Tae Sub Park, Cheol-Heui Yun(HRP)-conjugated secondary antibodies for one hour at room temperature. Goat anti-mouse IgG-HRP (Bio-Rad, 1706516) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen, 31460) were used as secondary antibodies. After five

(5) DNAJC12 Stabilizes Phenylalanine Hydroxylase and Facilitates Its Substrate‐Dependent ActivationFASEB JJuly 24, 2026Mary Dayne S. Tai, Trond‐André Kråkenes, Gloria Gamiz‐Arco, Christer F. Didriksen, Juha P. Kallio, Marte I. Flydal, Fernando Moro, Aurora Martinezanti‐rabbit antibody (1:1000 and 1:5000 for DNAJC12 and PAH detection, respectively; Bio‐Rad, cat. no. 1706515) for 1 h. The membrane was washed again prior to visualization using enhanced Luminata Immobilon Crescendo

(6) Anticancer effects of tangeretin associated with reactive oxygen species generation, mitochondrial dysfunction and apoptosis in CaSki cellsOncol RepJuly 24, 2026Seung-Hyeon Ahn, Zeeshan Ahmad Bhutta, Hwayoung Na, Hong Kyu Lee, Kyung-Chul Choihorseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:3,000; cat. no. 1706515; Bio-Rad Laboratories, Inc.) for 1 h at room temperature. Protein bands were visualized using an enhanced

(7) Thiol-Based Neuroprotective Copolymers Acutely Restore Redox Metabolism and Mediate Vasogenic Edema in a Mouse Model of Traumatic Brain InjuryMacromol BiosciJuly 21, 2026Evan T. Curtis, Brandon Z. McDonald, Aria W. Tarudji, Aaron M. Priester, Anthony J. Convertine, Forrest M. Kievitantibody,Host species,Antibody dilution,"Company, Catalog No." Anti‐Rabbit,Goat,1:10 000,"BioRad, 1705046" Anti‐Mouse,Goat,1:10 000,"BioRad, 1705047" Primary and secondary antibodies used for immunoblotting

(8) A newly developed eGFP-traceable recombinant rhesus lymphocryptovirus, a tool to study viral infection and replication in vitro and ex vivo , confirms gp350 as key for viral entryJournal of VirologyJuly 21, 2026Erasmus N. Kotey, Preeti Sharma, Esther Rodriguez, Jean Patrick Gonzalez-Dahua, Brenda A. Tello, Ivana G. Reidel, Gabriela M. Escalante, Yuqi Zhao, Xiwei Wu, Rodney P. Kincaid, et al.temperature with 100 µL of HRP-conjugated goat anti-mouse IgG secondary antibody (1:2,000 in PBS; BioRad 1706516). The plates were washed three times, and 100 µL ABTS 2-component microwell peroxidase chromogenic substrate

(9) Pharmacological modulation of p75 neurotrophin receptor in microglial cells improves resilience to rotenone cytotoxicityFront PharmacolJuly 20, 2026Alessio Valenza, Daniele Pensabene, Francesca Rendina, Maurizio Muzzi, Anna Fracassi, Sandra Moreno, Marco Segattoincubated for 1 h at RT with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse, 1706516, Bio-Rad Laboratories; anti-rabbit, 1706515, Bio-Rad Laboratories). Immunoreactive bands were detected

(10) Identification of novel drug-specific PARP inhibitor resistance mechanisms in ovarian cancer–implications for clinical practiceBritish Journal of CancerJuly 20, 2026Caitlin J. Macdonald, Amber McWhirter, Aparajitha Vaidyanathan, Michelle J. Ferguson, H. Christian Eberl, Euan A. Stronach, Lynne Sawers, Gillian SmithTBS-T before incubation in either HRP-goat anti-rabbit polyclonal secondary antibody (1:2000, BioRad, #1706515) or HRP-goat anti-mouse polyclonal antibody (1:2000, BioRad, #1706516). Blots were developed using an

(11) Red blood cell differentiation using canine-induced pluripotent stem cellsStem Cells Transl MedJuly 20, 2026Kazuto Kimura, Masaya Tsukamoto, Kohei Shishida, Hiroko Sugisaki, Jun Katahira, Miyuu Tanaka, Mitsuru Kuwamura, Amir Kol, Mika Okada, Minoru Iijima, et al.5 min each with TBST, followed by incubation with goat anti–rabbit HRP-conjugated antibody (1:3000, 1706515, Bio-Rad Laboratories) diluted in TBST containing 3% skim milk for 1 hr at room temperature. After incubation

(12) Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactomeiScienceJuly 17, 2026Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo, et al.goat anti-rabbit IgG,ThermoFisher,Cat# A21428; RRID: AB_2535849 HRP goat anti-mouse IgG,BioRad,Cat# 1706516; RRID: AB_2921252 HRP goat anti-rabbit IgG,BioRad,Cat# 1706515; RRID: AB_11125142 Duolink® In Situ

(13) The NLRP3 Inflammasome Increases Pulmonary Vascular Remodeling in Experimental Pulmonary Arterial HypertensionPulm CircJuly 17, 2026Emmanouil Mavrogiannis, Rebeca Weldeghebreal, Iris R. Schilthuis, Zain K. Fal, Niels J. Kloosterhuis, Mirjam H. Koster, Wim Timens, Johannes M. Douwes, Rolf M. F. Berger, Marit Westerterpanti‐GAPDH (1:10000) (ab8245, Abcam plc) were used as primary antibodies and anti‐rabbit IgG HRP (1:2000) (#1706515; Bio‐Rad Laboratories Inc.) or anti‐goat IgG HRP‐linked antibody (1:2000) (sc‐2020; Santa Cruz Biotechnology Inc

(14) Cepharanthine inhibits tick‑borne encephalitis virus infection through modulation of the stress and inflammation responseInt J Mol MedJuly 16, 2026Wan-Da Tang, Lan-Juan Zhaomembranes were incubated for 2 h with horseradish peroxidase-conjugated goat anti-mouse (1:2,000; cat. no. 1706516; Bio-Rad Laboratories, Inc.) or goat anti-rabbit secondary antibodies (1:2,000; cat. no. 7074; Cell Signaling Technology

(15) Proteome of CD9+ Plasma Small Extracellular Vesicles Differentiates Stages of HPV-Associated Cervical Neoplasia from Normal Epithelium to Invasive CancerLife (Basel)July 16, 2026Alexander M. Yurin, Natalia L. Starodubtseva, Anna E. Bugrova, Alexey S. Kononikhin, Denis N. Silachev, Vladimir E. Frankevich, Alisa O. Tokareva, Maria I. Indeykina, Ekaterina A. Evtushenko, Alexander A. Yakovlev, et al.horseradish peroxidase-conjugated secondary antibody (goat anti-rabbit IgG (H + L)-HRP conjugate, #1706515, Bio-Rad, Hercules, CA, USA) diluted 1:10,000. Membranes were washed again five times with TNT and developed

(16) Iron Deficiency-Induced Hair Loss Is Associated with ROS-Mediated Disruption of Wnt/β-Catenin SignalingNutrientsJuly 15, 2026Sang-Ah Kwon, So Young Bu, Yeon-Hee Kim, Joo Weon Lim, Christopher D. Vulpe, Seung-Min LeeMillipore-2884131, Burlington, MA, USA) or a secondary goat anti-mouse antibody (Bio-Rad Laboratories-1706516, Hercules, CA, USA) in PBS/0.05% Tween 20 containing 2.5% skim milk for 2 h. The blots were developed

(17) Individual contributions of exotoxins S and T on internalized P. aeruginosaInfection and ImmunityJuly 14, 2026Zachary J. Resko, Adam V. Thota, Christopher J. Corcoran, David G. Glanville, Seby Edassery, Keith A. Klein, Jonathan P. Allen, Jonathan A. Kirk, Andrew T. Ulijasz, Abby R. Krokenreagent (Millipore Sigma; MABE1076) and then 1:3,000 goat-α-rabbit IgG (H + L) HRP conjugate (Bio-Rad; #1706515). For detection of HA-tagged ExoS, membranes were incubated with 1:5,000 rat α-HA (Millipore Sigma;

(18) Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregatesInfection and ImmunityJuly 14, 2026Jonah Lanier, Jaden J. Skelly, Freddie Salsbury, Volkan K. Köseoğlu66006-2-Ig; 1:10,000) in 5% BSA-PBST, washed, and probed with HRP-conjugated goat anti-mouse IgG (BioRad, 1706516; 1:50,000) for 1 h at room temperature. Protein bands were visualized using Pierce ECL Western Blotting Substrate

(19) Comparative Analysis of Endothelial Cell Culture Models Under Altered Mechanical Conditions and Glucose VariationsInt J Mol SciJuly 13, 2026Augusta Volkevičiūtė, Jayashree Sahana, Estéfano Pinilla, Daniela Melnik, Luis Fernando González-Torres, Markus Wehland, Edgaras Stankevicius, Daniela Grimm, Ulf Simonsen(VE-Cadherin),Rabbit,CST,#2500S,WB,1:1000 Secondary,Goat Anti-rabbit (GAR)-HRP Conjugate,Goat anti-rabbit,Bio Rad,#1705046,WB,"1:10,000" Secondary,Anti-rabbit IgG (Alexa Fluor 488 Conjugate),Goat anti-rabbit,CST,#4412,IF,"1:10,000" Secondary,Anti-mouse

(20) Design and Immunogenicity of a Nanoparticle Combination Vaccine for Salmonella in PoultryPlant Biotechnol JJuly 13, 2026Shabnam Shamriz, Angelo Kaldis, Carly A. Charron, Jordan T. VanderBurgt, Christopher P. Garnham, Moussa S. Diarra, Rima Menassaanti‐mouse horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad, Hercules, USA, Cat. No. 1706516) diluted at 1:5000 in 0.5% blocking solution. Chemiluminescence was detected using Enhanced the Chemiluminescent

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9/13/2022Reliable Secondary Antibody with Clear Detection Arianna NapoliI used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ... Read Review

HRP and AP Conjugates

HRP and AP Conjugates from Bio-Rad

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HRP and AP Conjugates from Bio-Rad

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