HRP and AP Conjugates 1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403 from Bio-Rad

Product Specs
ItemHRP and AP Conjugates
CompanyBio-Rad
Price Supplier Page
Catalog Number1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
ApplicationsWestern Blotting

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

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Website: http://discover.bio-rad.com

(1) A comparative toxicological evaluation of nicotine and its analog 6-methyl nicotine in E-cigarette aerosol utilizing a 3D in vitro human respiratory modelToxicologyMay 1, 2026Felix Effah, Mohana Sengupta, Yehao Sun, Md Imam Faizan, Gagandeep Kaur, Rhea Raghu, Atallah Elzein, Tim Marczylo, Robert M. Strongin, Irfan Rahmantemperature with HRP-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Bio-Rad, Cat.1706515) at a 1:10,000 dilution. Following three 5-minute washes in TBS-T, immunoreactive bands were visualized

(2) Regulation of Endoplasmic Reticulum Stress by Empagliflozin in Doxorubicin-Induced Cardiotoxicity in RatsJ Cell Mol MedApril 29, 2026Akshi Malik, David C. Y. Cheung, D. Allison Ledingham, Danielle Desautels, Joerg Herrmann, Pawan K. Singal, Davinder S. Jassalwere probed with Horseradish peroxidase conjugated goat‐antirabbit/antisecondary antibody (Product #: 1706515, BioRad) for 60 min at room temperature before band visualisation using ChemDoc imager (BioRad, Canada).

(3) Cell-specific DNA methylation in human alpha and beta cells regulates gene expression in type 2 diabetesNat MetabApril 24, 2026Jones K. Ofori, Sabrina Ruhrmann, Axel Lindström, Alexander Perfilyev, Melina Martin, Alexandros Karagiannopoulos, Lucia Scisciola, Katja Kost, Josefine Jönsson, Åsa Nilsson, et al.peroxidase-conjugated goat anti-rabbit (7074, lot 32; 1:10,000 dilution, Cell Signaling Technology) or goat anti-mouse (1706516, 1:2,000 dilution, Bio-Rad). Clarity Western ECL Substrate was used for visualization of proteins with

(4) Enterovirus-induced cleavage of Mitofusin 2 generates mitophagosomes for enveloped virion releaseSci AdvApril 24, 2026Alagie Jassey, Bimal Paudel, Michael A. Wagner, Noah Pollack, I-Ting Cheng, Raquel Godoy-Ruiz, David J. Weber, William T. Jacksonperoxidase–conjugated anti-mouse secondary and anti-rabbit secondary antibodies were obtained from Bio-Rad (#1706516 and #1706515, respectively). Virus infections Viral infections were performed following previously

(5) TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory networkNat CommunApril 23, 2026Alina A. Walth-Hummel, Celine Jouffe, Peter Weber, Karsten Motzler, Julia Geppert, Michael Sterr, Wei Gan, Iwona Szczerbinska, Ann-Christine König, Stefanie M. Hauck, et al.After the secondary antibody incubation at room temperature for 1 h (goat anti-rabbit HRP, BioRad, Cat# 1705046, RRID: AB_11125757; goat anti-mouse HRP, BioRad, Cat# 1706516, RRID: AB_2921252; IgG Fraction Monoclonal

(6) Amelioration of gentamicin-induced acute kidney injury by trifluoperazine: in vivo mechanistic insightsSci RepApril 20, 2026Ahmed E. Goda, Nereen A. Almosilhy, Nageh A. El-MahdyCell Signaling Technology, USA). The secondary antibody used was goat anti-rabbit IgG-HRP conjugate (BioRad, USA, Cat# 1706515). The Enhanced Chemiluminescent kit used was UltraScence Pico Plus Western Substrate (BIO-HELIX Co.

(7) Chronic psychological stress disrupts liver homeostasis by dysregulating oxidative phosphorylation via the PI3K/AKT/FoxO3a axisiScienceApril 17, 2026Yue Wang, Rongjie Zhao, Ziyin Yuan, Lina Zhai, Xin Ning, Man Lv, Zihui Jin, Haroon Iqbal, Uzair Ur-Rehman, Zhou Yi, et al.14220T; RRID: AB_2798429 rabbit anti-Ki67,ABclonal,Cat# A26419PM goat anti-mouse HRP,Bio-rad,Cat#1706516; RRID: AB_2921252 goat anti-rabbit HRP,Bio-rad,Cat#1706515; RRID: AB_11125142 Alexa Fluor 488 goat

(8) Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2iScienceApril 17, 2026Haw-Chih Tai, Xiaoou Li, Christina J. Park, Altayeb Alshaikh, Brian Yu, Daiqing Chen, Phetcharat Chen, James K. LiaoPVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad; 1706515, 1706516; dilution: 1:5000). The protein bands were visualized with enhanced chemiluminescence (ECL)

(9) Matrix plasticity and the molecular basis of extracellular filament assembly in Bacillus cereusSci AdvApril 17, 2026Ana Álvarez-Mena, Muhammed Bilal Abdul Shukkoor, Joaquín Caro-Astorga, Mélanie Berbon, María Luisa Antequera-Gómez, Montserrat Grifé-Ruiz, Axelle Grélard, Brice Kauffmann, Estelle Morvan, Oscar P. Kuipers, et al.secondary antibody against rabbit immunoglobulin G (IgG) conjugated to horseradish peroxidase (Bio-Rad, 1706515) for 2 hours at a concentration of 1:3000 and diluted in TBS-T. Following the incubation, the membrane

(10) Neuronal SEL1L-HRD1 ER-associated degradation is essential for motor function and survival in miceJournal of Clinical InvestigationApril 15, 2026Mauricio Torres, You Lu, Brent Pederson, Hui Wang, Anna Gretzinger, Liangguang L. Lin, Jiwon Hwang, Xinxin Chen, Alan C. Rupp, Abigail J. Tomlinson, et al.After washing with TBST, membranes were incubated with HRP-conjugated secondary antibodies (Bio-Rad,1706515, 1:5,000) for 1 hour at room temperature. Signals were developed using an ECL chemiluminescence detection

(11) Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein responseBioact MaterApril 9, 2026Yu Sun, Qifeng Chen, Yan Zhao, Yuanyuan Yin, Qiao Chen, Lixin Yang, Zixuan Liu, Rui Chen, Xiaohui Xu, Jinlin Song(1:5000, ET1601-4, Hua-bio). After incubation with corresponding HRP-conjugated secondary antibodies (1706516 and 1706515, Bio-Rad), protein bands were detected using an enhanced chemiluminescence (ECL) kit (P0018AM,

(12) Audiogenic Kindling Stimulation Fails to Induce Cerebral Overexpression of P-Glycoprotein and Limbic Crises in the GASH/Sal Model of EpilepsyInt J Mol SciApril 9, 2026Laura Zeballos, Jerónimo Auzmendi, Alberto Lazarowski, Dolores E. Lópezwith a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (Bio-Rad, Cat. No. 1706515) diluted 1:3000. Protein bands were visualized using a chemiluminescent substrate, and signals were

(13) Identification and Validation of an Inhibitor of the Protein Kinases PIM and DYRKJournal of Medicinal ChemistryApril 9, 2026Gyula Bencze, Prabhadevi Venkataramani, Elad Elkayam, Keith D. Rivera, Ankur Garg, Istvan Szabadakai, Laszlo Orfi, Leemor Joshua-Tor, Darryl J. Pappin, Nicholas K. Tonksincubated with secondary antibodies for 1 h at room temperature. HRP goat antimouse (Bio-Rad; Cat. no. 1706516) at 1:50,000 was used for tubulin blots, while HRP goat antirabbit (Abcam, Cat. no. ab6721) at 1:30,000

(14) Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type TargetingACS Applied Materials & InterfacesApril 8, 2026Hyun Min Kim, Marjan Omer, Grant A. Knappe, Patrick McMullen, Duy An Le, Ashwin Pasupathy, Daniel G. Anderson, Mark Bathepurchased from Invitrogen. Horseradish peroxidase (HRP)-conjugated goat antimouse IgG secondary antibody (#1706516), iScript RT-qPCR Sample Preparation Reagent (#1708899) was purchased from BioRad. HiSpeed EndoFree

(15) Glioblastoma Cells Expressing Oncogenic EGFR Release Multiple Extracellular Vesicle Subpopulations Positive or Negative for EGFRJ Extracell BiolApril 7, 2026Elham Pishavar, Fereshteh Shojaei-Ghahrizjani, Brian Meehan, Nadim Tawil, Cristiana Spinelli, Dongsic Choi, Fang Cheng Wong, Mahsa Jalali, Janusz Rakantibodies, followed by horseradish peroxidase‐conjugated secondary antibodies (anti‐mouse, Bio‐Rad 1706516; anti‐rabbit, Cell Signalling 7074S). Chemiluminescence was detected using the Amersham ECL WB Detection

(16) Ovarian Insulin Signaling Is Intact Despite Systemic Insulin Resistance in a Mouse Model of Polycystic Ovary SyndromeEndocrinologyApril 7, 2026Adelaide E Weidner, Kenji Vann, Alexia I Hodowanec, Denise Ivey, Anna Roy, Zachary R Sechrist, Calvin L Cole, Olga Astapovahour at room temperature with secondary antibody (Bio-Rad # 170-6515, RRID:AB_11125142 or Bio-Rad # 1706516, RRID:AB_2921252). Band density was quantified using either ImageLab v6.1.0 build 7 (Bio-Rad) or ImageJ

(17) Genetic background shapes SEZ6L2 autoimmunity and reveals coordinated immune responses linked to neurological dysfunctionbioRxivApril 3, 2026Carlos J. Reyes-Sepúlveda, John Randolph, Julia M. Granato, Alison Hobbins, Jennetta W. Hammondserum samples (1:1000), and developed using horseradish peroxidase–conjugated anti-mouse IgGs (Bio-Rad, 1706516, 1:15,000), TMB substrate (ThermoScientific, N301), and 0.18 M sulfuric acid stop solution. Absorbance

(18) Modification of the dermal matrix by senescence associated lipids and its functional consequenceRedox BiologyApril 1, 2026Sarah Jelleschitz, Christopher Kremslehner, Ionela-Mariana Nagelreiter, Michael Mildner, Melanie Salek, Christina Bauer, Alexandra Stiegler, Adrian Sandgren Fors, Michaela Schirato, Christian Freystätter, et al.a dilution of 1:10 000 in 2% BSA (w/v) in PBS followed by a secondary anti-rabbit IgG-HRP (Bio-Rad 1706515, 1:10 000). 2.5 LC-MS/MS analysis of lipid modified collagen Lipid modified collagen was mixed with

(19) Spermidine Suppresses Peripheral Inflammation and Alleviates Non-Motor Symptoms in the 6-OHDA-Induced Rat Model of Parkinson’s DiseaseMoleculesMarch 31, 2026Beata Grembecka, Oliwia Harackiewicz, Jan Ruciński, Daria Korewo-Labelle, Ewelina Kurowska-Rucińska, Irena Majkutewiczanti-rabbit secondary antibody conjugated with horseradish peroxidase (HRP) (Biorad, Hercules, CA, USA, cat# 1706515, 1:500). The sections were rinsed three times with PBS and incubated in 0.075% diaminobenzidine tetrahydrochloride

(20) Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growthbioRxivMarch 27, 2026Nitzan Samra, Pabitra K. Sahoo, Agostina Di-Pizio, Courtney N. Buchanan, Nataliya Okladnikov, Ofri Abraham, Shifra Ben-Dor, Rebecca Haffner-Krausz, Ida Rishal, Jeffery L. Twiss, et al.secondary antibody (goat anti-mouse HRP (1:10,000) or goat anti-rabbit HRP (1:10,000), Bio-Rad cat. 1706516 and 1706515, respectively). The membrane was washed again in 1 × TBST for three times 15 min and chemiluminescence

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9/13/2022Reliable Secondary Antibody with Clear Detection Arianna NapoliI used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ... Read Review

HRP and AP Conjugates

HRP and AP Conjugates from Bio-Rad

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HRP and AP Conjugates from Bio-Rad

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