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HRP and AP Conjugates
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HRP and AP Conjugates
HRP and AP Conjugates from Bio-Rad
(1)
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Citations:
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Figures:
(2)
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Supplier Page from
Bio-Rad for
HRP and AP Conjugates
Description
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
HRP and AP Conjugates
Company
Bio-Rad
Price
Supplier Page
Catalog Number
1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
Applications
Western Blotting
Add to Compare List
Bio-Rad
Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive
Hercules
,
California
94547
United States
Phone
: 800-424 6723
800-879 2289
Company Profile
Website:
http://discover.bio-rad.com
Citations (1820)
(1)
Dopaminergic neurons are vulnerable to dysregulation of YEATS2-dependent calcium homeostasis
iScience
June 19, 2026
Luca Lo Piccolo, Ranchana Yeewa, Pitiporn Noisagul, Arnaud Monteil, Vorasuk Shotelersuk, Salinee Jantrapirom
AB_221569 Tyrosine hydroxylase (TH),
Merck
,
AB152
; RRID: AB_390204 Anti-mouse IgG (H + L)-HRP Conjugate,
Biorad
,Cat#
1706516
; RRID: AB_2921252 Anti-rabbit IgG (H + L)-HRP Conjugate,
Biorad
,Cat#
1706515
; RRID: AB_11125142
Goat anti-rabbit IgG
(2)
Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia
J Cell Biol
June 1, 2026
Ziyan Wang, Bradley M. Bartholomai, Bin Wang, David Ritz, Daniel Schultz, Jennifer J. Loros, Jay C. Dunlap
DM1A,
1:5000
Anti-V5 (
mouse
),Invitrogen #46–0705,
1:5000
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
,
BIO-RAD
#
1706515
,
1:5000
Goat Anti-Mouse IgG (H + L)-HRP Conjugate
,
BIO-RAD
#
1706516
,
1:5000
(3)
Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expression
Nucleic Acids Res
May 26, 2026
Zhiyun Yang, Zongchao Jia
temperature for 1 h with shaking. The secondary antibodies used were
goat
anti-rabbit HRP (
Bio-Rad
, #
1706515
), goat anti-mouse HRP (
Bio-Rad
, #
1721011
). Followed by washing the
membrane
three times with TBST at
(4)
Vespakinin-M delineates an AMPK/mTOR-arginine-TCA cycle axis to act as an immunometabolic switch in post-stroke microglia
Redox Biol
May 12, 2026
Dexiao Wang, Jingyu Zhang, Zhejun Zhuang, Qian Wang, Jie Li, Xue Wang, Kunkun Li, Yunyun Liu, Yanhui Cao, Lijuan Li,
et al.
10-min washes with TBST,
membranes
were incubated with HRP-conjugated
goat anti-rabbit IgG
(
1:3000
, #
1706515
,
Bio-Rad
, USA) for 1 h at RT. Protein bands were visualized using enhanced chemiluminescence substrate
(5)
Reticulate leaf venation in Pilea peperomioides is a Voronoi diagram
Nat Commun
May 12, 2026
CiCi Xingyu Zheng, Shirsa Palit, Matthew Venezia, Elijah Blum, Ullas V. Pedmale, Dave Jackson, Enrico Scarpella, Przemyslaw Prusinkiewicz, Saket Navlakha
TBST buffer and incubated with HRP-conjugated anti-rabbit secondary antibody (
BioRad
, catalog no. #
1706515
) (
1:10,000
dilution in TBST buffer supplemented with 1% BSA). After three washes in TBST buffer, proteins
(6)
HES3-dependent regulatory functions in development and fusion-positive rhabdomyosarcoma
bioRxiv
May 8, 2026
Jack Kucinski, Matthew R. Kent, Katherine M. Silvius, Ambuj Kumar, Cenny Taslim, Genevieve C. Kendall
(DSHB, PCRP-HES3–1A10).
1:10,000
HRP-α-rabbit (
Bio-Rad
,
1721019
) and
1:10,000
HRP-α-
mouse
(
Bio-Rad
,
1706516
) secondary antibodies were used for the corresponding primary antibody. Images were taken on a
ChemiDoc Go Imaging System
(7)
A comparative toxicological evaluation of nicotine and its analog 6-methyl nicotine in E-cigarette aerosol utilizing a 3D in vitro human respiratory model
Toxicology
May 1, 2026
Felix Effah, Mohana Sengupta, Yehao Sun, Md Imam Faizan, Gagandeep Kaur, Rhea Raghu, Atallah Elzein, Tim Marczylo, Robert M. Strongin, Irfan Rahman
temperature with HRP-conjugated
goat
anti-rabbit or
goat
anti-
mouse
secondary antibodies (
Bio-Rad
, Cat.
1706515
) at a
1:10,000
dilution. Following three 5-minute washes in TBS-T, immunoreactive bands were visualized
(8)
Gene regulatory co-option drives birdsong neural circuit specialization
bioRxiv
April 30, 2026
Arturo P. Marquez, Cayla R. M. Lagousis, Eva G. Katcher, Frank Rand, Keerthi Varadharaj, Michael S. Brainard, Bradley M. Colquitt
in TBST were performed and the blot was incubated with
goat
α-rabbit-AP secondary antibody (
BioRad
,
1706518
) at
1:3000
dilution for 2 hours on a room temperature shaker. The blot was developed using Immun-Star
(9)
GerAB residues predicted to interact with water based on MD simulations mediate germinosome stability in Bacillus subtilis spores
Frontiers in Microbiology
April 29, 2026
Longjiao Chen, Silke van Buuren, George Korza, Jocelyne Vreede, Peter Setlow, Stanley Brul
(20), washed three times with TBST, and probed with
goat
anti-rabbit HRP antibody (
1:2500
;
BIO-RAD
#
1706515
) for 1 h before visualization.
(10)
Regulation of Endoplasmic Reticulum Stress by Empagliflozin in Doxorubicin-Induced Cardiotoxicity in Rats
J Cell Mol Med
April 29, 2026
Akshi Malik, David C. Y. Cheung, D. Allison Ledingham, Danielle Desautels, Joerg Herrmann, Pawan K. Singal, Davinder S. Jassal
were probed with
Horseradish peroxidase
conjugated
goat
‐antirabbit/antisecondary antibody (Product #:
1706515
,
BioRad
) for 60 min at room temperature before band visualisation using ChemDoc imager (BioRad, Canada).
(11)
Enterovirus-induced cleavage of Mitofusin 2 generates mitophagosomes for enveloped virion release
Sci Adv
April 24, 2026
Alagie Jassey, Bimal Paudel, Michael A. Wagner, Noah Pollack, I-Ting Cheng, Raquel Godoy-Ruiz, David J. Weber, William T. Jackson
peroxidase–conjugated anti-
mouse
secondary and anti-rabbit secondary antibodies were obtained from
Bio-Rad
(#
1706516
and #
1706515
, respectively).
Virus infections
Viral infections
were performed following previously
(12)
Cell-specific DNA methylation in human alpha and beta cells regulates gene expression in type 2 diabetes
Nat Metab
April 24, 2026
Jones K. Ofori, Sabrina Ruhrmann, Axel Lindström, Alexander Perfilyev, Melina Martin, Alexandros Karagiannopoulos, Lucia Scisciola, Katja Kost, Josefine Jönsson, Åsa Nilsson,
et al.
peroxidase-conjugated
goat
anti-rabbit (
7074
, lot 32;
1:10,000
dilution,
Cell Signaling Technology
) or
goat
anti-
mouse
(
1706516
,
1:2,000
dilution,
Bio-Rad
). Clarity Western ECL Substrate was used for visualization of proteins with
(13)
TREM2 promotes adipogenesis of PDGFR-α+ adipose stem cells but is dispensable for adipose remodeling and metabolic health during diet-induced obesity
Front Endocrinol (Lausanne)
April 23, 2026
Anja Dobrijevic, Ana Korosec, Julia Stefanie Brunner, Lenka Matejovicova, Anna Gemza, Karin Lakovits, Hon Shing Lam, Aloña Agirre-Lizaso, Gernot Schabbauer, Sylvia Knapp,
et al.
temperature - anti-rabbit IgG (#
7074
,
Cell Signaling
, used at
1:1000
in 5% BSA/PBST) or
goat anti-mouse IgG
(#
1706516
,
Biorad
, used at
1:1000
in 5% BSA/PBST). Thereafter,
membranes
were washed again 3x 10 min with PBST
(14)
TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network
Nat Commun
April 23, 2026
Alina A. Walth-Hummel, Celine Jouffe, Peter Weber, Karsten Motzler, Julia Geppert, Michael Sterr, Wei Gan, Iwona Szczerbinska, Ann-Christine König, Stefanie M. Hauck,
et al.
After the secondary antibody
incubation
at room temperature for 1 h (
goat
anti-rabbit HRP,
BioRad
, Cat#
1705046
, RRID: AB_11125757; goat anti-mouse HRP,
BioRad
, Cat#
1706516
, RRID: AB_2921252; IgG Fraction Monoclonal
(15)
Amelioration of gentamicin-induced acute kidney injury by trifluoperazine: in vivo mechanistic insights
Sci Rep
April 20, 2026
Ahmed E. Goda, Nereen A. Almosilhy, Nageh A. El-Mahdy
Cell Signaling Technology
, USA). The secondary antibody used was
goat anti-rabbit IgG-HRP
conjugate (
BioRad
, USA, Cat#
1706515
). The Enhanced Chemiluminescent kit used was UltraScence Pico Plus Western Substrate (BIO-HELIX Co.
(16)
Placental ferroptosis and impaired fetal growth in symptomatic and asymptomatic SARS-CoV-2 infections
Clin Sci (Lond)
April 17, 2026
Yusmaris Cariaco, Abolfazl Nik-Akhtar, Marie-Eve Brien, Keir Menzies, Sylvie Girard, Shannon Bainbridge
incubated for 1 h at room temperature with anti-rabbit (
Cell Signaling
, #
7074
) or anti-
mouse
(
Bio-rad
#
1706516
) HRP-conjugated secondary antibodies. Protein bands were detected using Clarity/Clarity Max ECL substrates
(17)
Matrix plasticity and the molecular basis of extracellular filament assembly in Bacillus cereus
Sci Adv
April 17, 2026
Ana Álvarez-Mena, Muhammed Bilal Abdul Shukkoor, Joaquín Caro-Astorga, Mélanie Berbon, María Luisa Antequera-Gómez, Montserrat Grifé-Ruiz, Axelle Grélard, Brice Kauffmann, Estelle Morvan, Oscar P. Kuipers,
et al.
secondary antibody against rabbit immunoglobulin G (IgG) conjugated to
horseradish
peroxidase (
Bio-Rad
,
1706515
) for 2 hours at a concentration of
1:3000
and diluted in TBS-T. Following the
incubation
, the
membrane
(18)
Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2
iScience
April 17, 2026
Haw-Chih Tai, Xiaoou Li, Christina J. Park, Altayeb Alshaikh, Brian Yu, Daiqing Chen, Phetcharat Chen, James K. Liao
PVDF
membranes
were incubated with
horseradish
peroxidase-conjugated secondary antibodies (
Bio-Rad
;
1706515
,
1706516
; dilution:
1:5000
). The protein bands were visualized with enhanced chemiluminescence (ECL)
(19)
Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio
Cell Death Discovery
April 17, 2026
Tommaso Pagliarusco, Anais Franco-Romero, Francesca Terrin, Filippo Citton, Ludovica Carducci, Nicola Facchinello, Camilla Maria Fontana, Marta Giacomazzo, Ranieri Verin, Maria Berica Rasotto,
et al.
(
PA1-16930
,
ThermoFisher Scientific
), and secondary antibodies:
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
(#
1706515
,
Bio-Rad
, Milan, Italy),
Goat Anti-Mouse IgG (H + L)-HRP Conjugate
(#
1706516
,
Bio-Rad
). Birefringence
(20)
Chronic psychological stress disrupts liver homeostasis by dysregulating oxidative phosphorylation via the PI3K/AKT/FoxO3a axis
iScience
April 17, 2026
Yue Wang, Rongjie Zhao, Ziyin Yuan, Lina Zhai, Xin Ning, Man Lv, Zihui Jin, Haroon Iqbal, Uzair Ur-Rehman, Zhou Yi,
et al.
14220T
; RRID: AB_2798429 rabbit anti-Ki67,ABclonal,Cat# A26419PM goat anti-mouse HRP,
Bio-rad
,Cat#
1706516
; RRID: AB_2921252
goat
anti-rabbit HRP,
Bio-rad
,Cat#
1706515
; RRID: AB_11125142 Alexa Fluor 488 goat
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Reviews
Write a Review
(1)
9/13/2022
Reliable Secondary Antibody with Clear Detection
Arianna Napoli
I used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ...
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HRP and AP Conjugates from Bio-Rad
Supplier Page
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