HRP and AP Conjugates 1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403 from Bio-Rad

Product Specs
ItemHRP and AP Conjugates
CompanyBio-Rad
Price Supplier Page
Catalog Number1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
ApplicationsWestern Blotting

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

(1) Genetic variants of the transporter SLC22A4 affect the abundance and survival of Fusobacterium nucleatum in colorectal cancerGut MicrobesDecember 31, 2026Samah Chouaibi, Veronica Fertitta, Silvia Porreca, Leila Njim, Antonella Carcagnì, Gabriele Toietta, Gianluca Canettieri, Khadija Zouari, Maha Mastouri, Yosr Kadri, et al.anti-mouse IgG (H + L) and goat anti-rabbit IgG (H + L) antisera were obtained from Bio-Rad (cat. #1706516 and #1706515, respectively). Cell Infection in vitro and Gentamicin protection assay Spheroids were

(2) Optimizing plasma-derived small extracellular vesicle purification to enhance downstream applicationsiScienceSeptember 18, 2026Fanxiu Ma, Meng Yuan, Yue Zhao, Xiumei Jin, Chengsi Wu, Xin Fang, Zhaodi Jiang, Shuying Liu, Wen-Quan Zou, Zhen Li, et al.subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Biorad # 1706515, 1706516), and the immunoreactive proteins were detected using an ECL kit (Tannon #180–501). Afterward,

(3) Therapeutic rescue of pathogenic asparaginyl-tRNA synthetase allelesMolecular Therapy Nucleic AcidsSeptember 8, 2026Tristan N. Samuels, Jenica H. Kakadia, Cian Ward, Fatema Abdullatif, Marisa I. Mendes, Desiree E. Smith, Rachel Heilmann, Fanqi Wu, Jakub M. Swiercz, Gajja S. Salamons, et al.Secondary antibodies IRDye 800CW Donkey anti-rabbit IgG, goat anti-mouse IgG (H + L)-HRP conjugate (#1706516, Bio-Rad), and 600CW goat anti-mouse (LI-COR) were used at 1:5,000 and 1:10,000 dilutions, respectively.

(4) Nanoscale junctional membrane curvatures recruit BIN1 and SNX9 for endothelial collective migrationJ Cell BiolSeptember 7, 2026Vera Janssen, Hannah de Kraker, Jesse S. Aaron, Annett de Haan, Iris de Heer, Satya Khuon, Jason Da Silva, Amber J.M. Driessen, Teng-Leong Chew, Josephine M.E. Tan, et al.For western blotting, secondary anti-mouse and rabbit antibodies coupled to HRP were obtained from Bio-Rad (Cat #1706515, #1721011, 1:1,000 for WB). Western blots were imaged using enhanced chemiluminescence detection (#34580;

(5) Differential toll-like receptor signaling pathways shape IL-1β-dependent inflammatory responses in chicken macrophagesPoultry ScienceSeptember 1, 2026Seungyoon Roh, Tina Sørensen Dalgaard, Ngo Thuy Bao Tran, Tae Sub Park, Cheol-Heui Yun(HRP)-conjugated secondary antibodies for one hour at room temperature. Goat anti-mouse IgG-HRP (Bio-Rad, 1706516) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen, 31460) were used as secondary antibodies. After five

(6) Fibrin Degradation Products Regulate Endothelial Barrier Function in Traumatic Brain Injury via the Actin CytoskeletonNeurotrauma RepAugust 26, 2026Ayhan Atmanli, Aidan Michael Collier, Scott Douglas Olson, Charles Samuel Cox(MA5-15738, Thermo Fisher, 1:4000 dilution) antibodies at 4°C overnight. Goat anti-mouse HRP antibody (1706516, Bio-Rad Laboratories, 1:5000 dilution) and goat anti-rabbit HRP antibody (1706515, Bio-Rad Laboratories,

(7) Squash enables Spindle-E-dependent heterotypic piRNA amplification in the Drosophila ovaryiScienceAugust 21, 2026Xin Xu, Taichiro Iki, Shinichi Kawaguchi, Toshie KaiHRP-conjugated goat anti-rat (1:1000, Dako, Cat. #P0450), HRP-conjugated goat anti-mouse (1:3000, BioRad, Cat. # 1706516) and HRP-conjugated goat anti-rabbit (1:3000, BioRad, Cat. #1706515). ANTI-FLAG M2-Peroxidase (1:1000,

(8) Multi-Omics Analysis of PBMCs Revealed Distinct Biological Differences Between Hemodialysis and Peritoneal DialysisInt J Mol SciAugust 20, 2026Maurizio Bruschi, Simona Granata, Silvia Lai, Valentina Pistolesi, Laura Barberio, Rossana D'Agostino, Francesca Sorace, Francesca Giordano, Paola Pontrelli, Loreto Gesualdo, et al.membranes were incubated with an HRP-conjugated goat anti-rabbit IgG secondary antibody (Bio-Rad, Cat# 1706515) for 2 h at room temperature. Following additional washes, signal detection was performed using the

(9) Identification and characterization of quorum-quenching enzyme AiiF from Acidithiobacillus ferrooxidans and its application for biofilm disruptionApplied and Environmental MicrobiologyAugust 19, 2026Mallori A. Herishko, Hannah S. Zurier, Scott Bantawere used at a 1:4,000 dilution, and secondary antibodies (Bio-Rad, Immun-Star GAM-HRP conjugate, #1705047) were used at a 1:10,000 dilution. Approximately 20 µg of protein was loaded per lane. Protein concentration

(10) Dynamic NFIX Promoter Usage Shapes Stem and Committed Myogenic StatesInt J Mol SciAugust 18, 2026Gabriele Rovetta, Giulia Ferrari, Mirko Ronzio, Alberto Gallo, Raffaele Epis, Chiara Bonfanti, Diletta Dolfini, Giorgia Careccia, Graziella Messinaantibodies (1:10,000, anti-mouse or anti-rabbit, IgG-HRP, Bio-Rad Laboratories, Hercules, CA, USA; 1706516, 1706515) at room temperature for 45 min in 5% Milk TBST 1X. Protein bands were revealed through ECL

(11) Differential expression of TGF-β1, TGF-β2, and TGF-β3 across WHO grades in meningioma: convergent evidence from mRNA, methylation, miRNA, and protein analysisJ NeurooncolAugust 17, 2026Mateusz Miller, Damian Strojny, Dawid Sobański, Rafał Staszkiewicz, Paweł Gogol, Wiktoria Kucybała, Beniamin Oskar Grabarekantibodies were visualized with an HRP-conjugated goat anti-rabbit IgG secondary antibody (catalog no. 1706515, Bio-Rad, Milan, Italy; 1:3000). For ELISA, absorbance was read at 540 nm (Tecan M200PRO), with recombinant

(12) TSPO Modulation by PIGA-1138 Attenuates Oxidative Stress and Preserves Retinal Function in Experimental Diabetic RetinopathyAntioxidants (Basel)August 12, 2026Alessia Galante, Francesca Corsi, Rosario Amato, Sabrina Taliani, Federico Da Settimo, Maurizio Cammalleri, Ilaria Piano, Massimo Dal Monte, Claudia Garginihorseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Bio-Rad Laboratories, Cat. No. 1706515). After incubation, immunoreactive bands were detected using Clarity™ Western ECL Substrate (Bio-Rad

(13) Structure of NHE6 and its lipid-mediated interactions regulating endosomal pHNat CommunAugust 11, 2026Sukkyeong Jung, Hyunku Yeo, Hang Li, Surabhi Kokane, Tom Reichenbach, Ashutosh Gulati, Giuseppe Albano, Carla Kirschbaum, Tin Manh Ho, Michael Landreh, et al.HRP-coupled goat anti-rat IgG antibody (#31430, Invitrogen) was used at a 1:2000 dilution, while avidin-HRP (#1706528, Bio-Rad) was used at a 1:1000 dilution. NHE1, NHE6 and NHE6-K351A transport activity NHE activity

(14) Polymerized Z-α-1 antitrypsin leads to lung injury in a murine model of emphysemaJCI InsightAugust 10, 2026Maria Magallón Serrano, Nazli Khodayari, William Bowers, Edward P. Manning, Xinran Liu, Jungnam Lee, Tammy O. Flagg, Regina Oshins, Aidan Griffin, Sahil Patel, et al.isolated from plasma at 55˚C for 18 hours, and goat anti-rabbit IgG (H+L)-HRP Conjugate (BioRad, Cat # 1706515) serving as detection antibodies were used to measure human Z-AAT polymers in plasma samples from mice,

(15) Non-Polio Enterovirus A71 and D68 Infection of Human Neuromuscular Organoids Reveals Distinct Mechanisms of Neuromuscular ImpairmentVirusesAugust 4, 2026Amber J. Schotting, Inés García-Rodríguez, Eline Freeze, Anoop T. Ambikan, Michael Wagner, Mira Mioch, Aymeric P. Y. L. Moffelein, William Jackson, Dasja Pajkrt, Katja C. Wolthers, et al.USA",836304,1:2000 HSP60,Rabbit,"Abcam, Cambridge, UK",AB46798,1:2000 Anti-Mouse HRP,Goat,"Bio-Rad Laboratories, Hercules, CA, USA",1706516,1:2000 Anti-Rabbit HRP,Goat,"Bio-Rad Laboratories, Hercules, CA, USA",1706515,1:2000 List of the

(16) RSAD2/VIPERIN and CMPK2 coordinate an immunometabolic response to Epstein-Barr VirusPLoS PathogAugust 3, 2026Urvi S. Zankharia, Adam M. Glass, Wujuan Zhang, Qing Zhu, Janvhi Suresh Machhar, Ying Ye, Bhanu Chandra Karisetty, Jayamanna Wickramasinghe, Andrew Kossenkov, Jozef Madzo, et al.9956T). Membranes were washed with TBST, incubated for 1 h with the goat anti-rabbit IgG-HRP (Biorad, 1706515) or goat anti-mouse IgG-HRP (Biorad, 1706516). Membranes were then washed and detected by enhanced chemiluminescence

(17) Inhibitory mechanism of ANP on aldosterone production in human adrenocortical cellsEndocr ConnectJuly 31, 2026Noriko Morishima, Atsuhiro Ichihara, Daisuke Watanabe, Noriyoshi Takano, Satoshi Morimoto, Yasufumi Sekithe appropriate horseradish peroxidase-conjugated secondary antibody (anti-rabbit IgG-HRP, Bio-Rad, 1706515; anti-mouse IgG-HRP, Proteintech, SA00001-1) and Chemi-Lumi One ECL detection kit (Nacalai Tesque Inc.,

(18) Macropinocytosis of Amyloid Precursor Protein Is Regulated by the Recruitment and Activity of Fe65, Arf6 and Rho GTPasesCellsJuly 29, 2026Jordan M. Krupa, Manoj Reddy Medapati, Abdul M. Naqvi, Ryan D. Hallam, Adrianna R. Tsang, Claudia Seah, Shawn N. Whitehead, Stephen H. Pasternakantibody (D12G6, 5740, Danvers, Cell Signaling, Danvers, MA, USA), Goat Anti-Rabbit-HRP conjugate (1706515, Bio-Rad, Mississauga, ON, Canada), and rabbit anti-GAPDH antibody (14C10, 2118, 1:1000, Cell Signaling,

(19) HSV-1 Infection Differentially Modulates NPY and VIP Neuropeptide Expression in the Mouse Brain and in Human Neuronal CellsInternational Journal of Molecular SciencesJuly 28, 2026Javier Carbone-Schellman, Nicolás Sales-Salinas, Rodrigo Reyes-Ramírez, Benjamín Rodríguez, Patricia Pereira-Sánchez, Gisella Vásquez-Canales, José A. Jara, Alfredo Molina-Berríos, Boris Rebolledo-Jaramillo, Alexis M. Kalergis, et al.R&D Systems, Minneapolis, MN, USA, CAT #HAF017; and 1:20,000, Anti-mouse, Bio-Rad, Hercules, CA, USA, CAT #1706516). After incubation, the membranes were washed with TBS-T (0.01%) three times for 5 min. Following membrane

(20) DNAJC12 Stabilizes Phenylalanine Hydroxylase and Facilitates Its Substrate‐Dependent ActivationFASEB JJuly 24, 2026Mary Dayne S. Tai, Trond‐André Kråkenes, Gloria Gamiz‐Arco, Christer F. Didriksen, Juha P. Kallio, Marte I. Flydal, Fernando Moro, Aurora Martinezanti‐rabbit antibody (1:1000 and 1:5000 for DNAJC12 and PAH detection, respectively; Bio‐Rad, cat. no. 1706515) for 1 h. The membrane was washed again prior to visualization using enhanced Luminata Immobilon Crescendo

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9/13/2022Reliable Secondary Antibody with Clear Detection Arianna NapoliI used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ... Read Review

HRP and AP Conjugates

HRP and AP Conjugates from Bio-Rad

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HRP and AP Conjugates from Bio-Rad

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