HRP and AP Conjugates 1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403 from Bio-Rad

Product Specs
ItemHRP and AP Conjugates
CompanyBio-Rad
Price Supplier Page
Catalog Number1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
ApplicationsWestern Blotting

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

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Phone: 800-424 6723
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Website: http://discover.bio-rad.com

(1) Genetic variants of the transporter SLC22A4 affect the abundance and survival of Fusobacterium nucleatum in colorectal cancerGut MicrobesDecember 31, 2026Samah Chouaibi, Veronica Fertitta, Silvia Porreca, Leila Njim, Antonella Carcagnì, Gabriele Toietta, Gianluca Canettieri, Khadija Zouari, Maha Mastouri, Yosr Kadri, et al.anti-mouse IgG (H + L) and goat anti-rabbit IgG (H + L) antisera were obtained from Bio-Rad (cat. #1706516 and #1706515, respectively). Cell Infection in vitro and Gentamicin protection assay Spheroids were

(2) Nanoscale junctional membrane curvatures recruit BIN1 and SNX9 for endothelial collective migrationJ Cell BiolSeptember 7, 2026Vera Janssen, Hannah de Kraker, Jesse S. Aaron, Annett de Haan, Iris de Heer, Satya Khuon, Jason Da Silva, Amber J.M. Driessen, Teng-Leong Chew, Josephine M.E. Tan, et al.For western blotting, secondary anti-mouse and rabbit antibodies coupled to HRP were obtained from Bio-Rad (Cat #1706515, #1721011, 1:1,000 for WB). Western blots were imaged using enhanced chemiluminescence detection (#34580;

(3) Anticancer effects of tangeretin associated with reactive oxygen species generation, mitochondrial dysfunction and apoptosis in CaSki cellsOncol RepJuly 24, 2026Seung-Hyeon Ahn, Zeeshan Ahmad Bhutta, Hwayoung Na, Hong Kyu Lee, Kyung-Chul Choihorseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:3,000; cat. no. 1706515; Bio-Rad Laboratories, Inc.) for 1 h at room temperature. Protein bands were visualized using an enhanced

(4) A newly developed eGFP-traceable recombinant rhesus lymphocryptovirus, a tool to study viral infection and replication in vitro and ex vivo , confirms gp350 as key for viral entryJournal of VirologyJuly 21, 2026Erasmus N. Kotey, Preeti Sharma, Esther Rodriguez, Jean Patrick Gonzalez-Dahua, Brenda A. Tello, Ivana G. Reidel, Gabriela M. Escalante, Yuqi Zhao, Xiwei Wu, Rodney P. Kincaid, et al.temperature with 100 µL of HRP-conjugated goat anti-mouse IgG secondary antibody (1:2,000 in PBS; BioRad 1706516). The plates were washed three times, and 100 µL ABTS 2-component microwell peroxidase chromogenic substrate

(5) Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactomeiScienceJuly 17, 2026Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo, et al.goat anti-rabbit IgG,ThermoFisher,Cat# A21428; RRID: AB_2535849 HRP goat anti-mouse IgG,BioRad,Cat# 1706516; RRID: AB_2921252 HRP goat anti-rabbit IgG,BioRad,Cat# 1706515; RRID: AB_11125142 Duolink® In Situ

(6) Cepharanthine inhibits tick‑borne encephalitis virus infection through modulation of the stress and inflammation responseInt J Mol MedJuly 16, 2026Wan-Da Tang, Lan-Juan Zhaomembranes were incubated for 2 h with horseradish peroxidase-conjugated goat anti-mouse (1:2,000; cat. no. 1706516; Bio-Rad Laboratories, Inc.) or goat anti-rabbit secondary antibodies (1:2,000; cat. no. 7074; Cell Signaling Technology

(7) Proteome of CD9+ Plasma Small Extracellular Vesicles Differentiates Stages of HPV-Associated Cervical Neoplasia from Normal Epithelium to Invasive CancerLife (Basel)July 16, 2026Alexander M. Yurin, Natalia L. Starodubtseva, Anna E. Bugrova, Alexey S. Kononikhin, Denis N. Silachev, Vladimir E. Frankevich, Alisa O. Tokareva, Maria I. Indeykina, Ekaterina A. Evtushenko, Alexander A. Yakovlev, et al.horseradish peroxidase-conjugated secondary antibody (goat anti-rabbit IgG (H + L)-HRP conjugate, #1706515, Bio-Rad, Hercules, CA, USA) diluted 1:10,000. Membranes were washed again five times with TNT and developed

(8) Iron Deficiency-Induced Hair Loss Is Associated with ROS-Mediated Disruption of Wnt/β-Catenin SignalingNutrientsJuly 15, 2026Sang-Ah Kwon, So Young Bu, Yeon-Hee Kim, Joo Weon Lim, Christopher D. Vulpe, Seung-Min LeeMillipore-2884131, Burlington, MA, USA) or a secondary goat anti-mouse antibody (Bio-Rad Laboratories-1706516, Hercules, CA, USA) in PBS/0.05% Tween 20 containing 2.5% skim milk for 2 h. The blots were developed

(9) Individual contributions of exotoxins S and T on internalized P. aeruginosaInfection and ImmunityJuly 14, 2026Zachary J. Resko, Adam V. Thota, Christopher J. Corcoran, David G. Glanville, Seby Edassery, Keith A. Klein, Jonathan P. Allen, Jonathan A. Kirk, Andrew T. Ulijasz, Abby R. Krokenreagent (Millipore Sigma; MABE1076) and then 1:3,000 goat-α-rabbit IgG (H + L) HRP conjugate (Bio-Rad; #1706515). For detection of HA-tagged ExoS, membranes were incubated with 1:5,000 rat α-HA (Millipore Sigma;

(10) Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregatesInfection and ImmunityJuly 14, 2026Jonah Lanier, Jaden J. Skelly, Freddie Salsbury, Volkan K. Köseoğlu66006-2-Ig; 1:10,000) in 5% BSA-PBST, washed, and probed with HRP-conjugated goat anti-mouse IgG (BioRad, 1706516; 1:50,000) for 1 h at room temperature. Protein bands were visualized using Pierce ECL Western Blotting Substrate

(11) Microglial Epigenetic Memory is Associated with Accelerated Resolution of Inflammatory Pain Induced by Prophylactic Macrophage-Derived Small Extracellular VesiclesJ Inflamm ResJuly 9, 2026Xuan Luo, Jason R Wickman, Jason T DaCunza, Yuzhen Tian, Ahmet Sacan, Seena K AjitSecondary HRP-conjugated antibodies used were Goat anti-rabbit IgG-HRP and Goat anti-mouse IgG-HRP (#1706515 and #1706516, Bio-Rad). Detection of Immune Surface Markers of sEVs via Flow Cytometry A bead-based

(12) Saccharomyces boulardii CNCM I-745 protects against small intestinal injury in a model of gliadin-peptide-induced sterile inflammatory enteropathyFront ImmunolJuly 6, 2026Aluminé Lezcano, Emanuel Miculan, Geronimo Ducca, Carolina Ruera, María Luz Iribarren, Philippe Tixador, Xavier Roux, Fernando Gabriel Chirdoab6789, Abcam) or horse-radish-peroxidase-conjugated secondary goat anti-rabbit antibody (1/2000 Cat# 1706515, BioRad) at 37°C for 1 h, and visualized using the enhanced chemiluminescent reagent (RPN2232, Amersham

(13) A carotenoid-rich functional tomato sauce (OsteoCol®) reduces liver fat content in adults with metabolic dysfunction-associated steatotic liver disease: a randomized clinical trialEur J NutrJuly 3, 2026Rosario Mare, Yvelise Ferro, Samantha Maurotti, Elisa Mazza, Alberto Castagna, Carmelo Pujia, Francesca Rita Noto, Angelo Galluccio, Angela Mirarchi, Giuseppe Andrea Giampà, et al.PPARα (MAB3890, MILLIPORE); SIRT1 (H00023411-M01, AbNOVA); β-ACTIN (A5441, SIGMA-ALDRICH); ANTI-MOUSE (#1706516, BIORAD); ANTI-RABBIT (#1706515, BIORAD). HMG-CR inhibitory activity assay The HMG-CoA Reductase Assay Kit

(14) Predicting Clinical Sensitivities of PDGFRA Exon 18 Mutations to Imatinib and Avapritinib to Optimize Gastrointestinal Stromal Tumor TreatmentCancer Res CommunJuly 1, 2026Homma M. Khosroyani, Alina Teuber, Ajia Town, Lillian R. Klug, Denisse Evans, Jerry Call, Sara Rothschild, Neeta Somaiah, Prapassorn Thirasastr, Mengyuan Liu, et al.antibodies were used at 1:1,000 dilutions in 5% BSA. Secondary mouse–horseradish peroxidase (HRP; Bio-Rad #1706516 RRID: AB_11125547) and rabbit-HRP (Bio-Rad #1706515 RRID: AB_11125142) conjugated antibodies, at 1:5,000

(15) Mice with humanized FXR ligand-binding domain display distinct metabolic responses upon pharmacological FXR stimulationJournal of Lipid ResearchJune 25, 2026Jinxiao Li, Hilde D. de Vries, Kirill Ustyantsev, Milaine V. Hovingh, Niels L. Mulder, Rick Havinga, Nicolette Huijkman, Sarah Falcone, Krisztina de Bruyn, Ellen Weersing, et al.washed with TBST and incubated with HRP-conjugated secondary antibody goat anti-mouse IgG/HRP (1:2000; #1706516, Bio-Rad) or goat anti-rabbit IgG/HRP (1:10,000; #P0448, DAKO) for 1 h at room temperature. Immune complexes

(16) Exploring the bioavailability and bioactivity of thiamine versus allithiamine: studies in Drosophila melanogaster and miceFrontiers in NutritionJune 24, 2026Sandra Nevermann, Hannah Weiß, Alexandra Fischer, Kai Lüersen, Keita Chikamoto, Daisuke Nakata, Yoshiyuki Ishida, Takahiro Furune, Keiji Terao, Gerald Rimbach1:250, ab110413, Abcam Limited, United Kingdom), followed by a secondary antibody (anti-rabbit, 1:4000, 1705046, Bio-Rad; anti-mouse, 1:4000, 1705047, Bio-Rad). For detection of total and phosphorylated PDHA1, membranes

(17) Bypass of LANA-DNA crosslinks by Polη ensures KSHV genome maintenance and tumor growthCell ReportsJune 23, 2026Jayaraju Dheekollu, Coltin J. Albitz, Anjali Pathania, Frederick Keeney, Samantha S. Soldan, Paul M. LiebermanMouse IgG,SCBT Inc,Cat# sc-2025 Anti-Rabbit IgG,Abcam,Cat# ab6721 Anti-Rabbit IgG HRP,Bio-Rad,Cat# 1706515 Anti-Mouse IgG HRP,Bio-Rad,Cat# 1706516 Ati-Rat IgG ATTO594,Rockland,Cat# 612-155-120 Ati-Rabbit IgG

(18) Antisense RNA controls the degradation of phycobilisomes in heterocyst-forming cyanobacteria by a transcriptional interference mechanismNucleic Acids ResJune 22, 2026Isidro Álvarez-Escribano, Manuel Brenes-Álvarez, Jens Georg, Agustín Vioque, Alicia M Muro-Pastor(Immobilon Western Chemiluminescent HRP Substrate, Merck) was used to detect the different antibodies, using anti-mouse 1:3000 (Bio-Rad #1706516) horseradish peroxidase-conjugated secondary antibodies in the case of anti-GFP. Ponceau Red staining

(19) Systemic AAV-hGCDH Gene Therapy Alleviates Glutaric Acid Accumulation and Attenuates Chronic Brain Vacuolation in a Novel Mouse Model of Glutaric Aciduria Type IInt J Mol SciJune 20, 2026Su Jin Kim, Yu Hwa Nam, Eun Young Joo, Jisun Park, Saeyoung Park, Sung-Chul Jung, Dong-Kyu Jin(7074S, Cell Signaling, Danvers, MA, USA) and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (1:10,000) (#1706516, Bio-Rad Laboratories, Inc., Hercules, CA, USA), and were incubated at a dilution of 1:10,000 for 1

(20) Soluble CD95L triggers Caspase-10-driven reactive oxygen species production in neutrophils and aggravates anti-neutrophil cytoplasmic antibody-vasculitisNature CommunicationsJune 20, 2026Andréa Boizard-Moracchini, Dhouha Msalbi, Sarah Huot-Marchand, Layla Haymour, Eden Lebrault, John Tchen, Titia Jekel, Gaël Galli, Benoît Brilland, Nathalie Mérillon, et al.incubated with the appropriate secondary antibody conjugated to horseradish peroxidase (anti-mouse IgG, #1706516, Bio-Rad, France) for 1 h at room temperature. Protein bands were visualized following exposure of the

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9/13/2022Reliable Secondary Antibody with Clear Detection Arianna NapoliI used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ... Read Review

HRP and AP Conjugates

HRP and AP Conjugates from Bio-Rad

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HRP and AP Conjugates from Bio-Rad

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