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HRP and AP Conjugates
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HRP and AP Conjugates
HRP and AP Conjugates from Bio-Rad
(1)
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Citations:
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Figures:
(2)
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Supplier Page from
Bio-Rad for
HRP and AP Conjugates
Description
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
HRP and AP Conjugates
Company
Bio-Rad
Price
Supplier Page
Catalog Number
1706515 / 1706516 / 1721050 / 1706522 / 1706425 / 1706528 / 1705047 / 1705046 / 1706518 / 1706520 / 1706521 / 1706533 / 1706403
Applications
Western Blotting
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Bio-Rad
Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive
Hercules
,
California
94547
United States
Phone
: 800-424 6723
800-879 2289
Company Profile
Website:
http://discover.bio-rad.com
Citations (1849)
(1)
Genetic variants of the transporter SLC22A4 affect the abundance and survival of Fusobacterium nucleatum in colorectal cancer
Gut Microbes
December 31, 2026
Samah Chouaibi, Veronica Fertitta, Silvia Porreca, Leila Njim, Antonella Carcagnì, Gabriele Toietta, Gianluca Canettieri, Khadija Zouari, Maha Mastouri, Yosr Kadri,
et al.
anti-mouse IgG (H + L) and
goat anti-rabbit IgG (H + L
) antisera were obtained from
Bio-Rad
(cat. #
1706516
and #
1706515
, respectively). Cell
Infection
in vitro
and Gentamicin protection
assay
Spheroids were
(2)
Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactome
iScience
July 17, 2026
Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo,
et al.
goat anti-rabbit IgG,
ThermoFisher
,Cat#
A21428
; RRID: AB_2535849 HRP goat anti-mouse IgG,
BioRad
,Cat#
1706516
; RRID: AB_2921252 HRP goat anti-rabbit IgG,
BioRad
,Cat#
1706515
; RRID: AB_11125142 Duolink® In Situ
(3)
Antisense RNA controls the degradation of phycobilisomes in heterocyst-forming cyanobacteria by a transcriptional interference mechanism
Nucleic Acids Res
June 22, 2026
Isidro Álvarez-Escribano, Manuel Brenes-Álvarez, Jens Georg, Agustín Vioque, Alicia M Muro-Pastor
(
Immobilon Western Chemiluminescent HRP Substrate
,
Merck
) was used to detect the different antibodies, using anti-
mouse
1:3000
(
Bio-Rad
#
1706516
)
horseradish
peroxidase-conjugated secondary antibodies in the case of anti-GFP. Ponceau Red
staining
(4)
Systemic AAV-hGCDH Gene Therapy Alleviates Glutaric Acid Accumulation and Attenuates Chronic Brain Vacuolation in a Novel Mouse Model of Glutaric Aciduria Type I
Int J Mol Sci
June 20, 2026
Su Jin Kim, Yu Hwa Nam, Eun Young Joo, Jisun Park, Saeyoung Park, Sung-Chul Jung, Dong-Kyu Jin
(
7074S
,
Cell Signaling
, Danvers, MA, USA) and
Goat Anti-Mouse IgG (H + L)-HRP Conjugate
(
1:10,000
) (#
1706516
,
Bio-Rad Laboratories
, Inc., Hercules, CA, USA), and were incubated at a dilution of
1:10,000
for 1
(5)
Neuronal microexons modulate arousal via the cAMP-PKA-CREB pathway in zebrafish
Sci Adv
June 19, 2026
Tahnee Mackensen, Luis Pedro Iñiguez, Thomas Soares Mullen, Cristina Rodriguez-Marin, François Kroll, Giulia Zuccarini, Jordi Fernandez-Albert, Laia Sancho-Vila, Jon Permanyer, Isaac H. Bianco,
et al.
(Supplementary Materials). Horseradish peroxidase (HRP)–conjugated secondaries [
goat
anti-rabbit (
Bio-Rad
,
1706515
),
1:10,000
;
sheep
anti-
mouse
(Cytiva NA931V),
1:5000
] were used. Signal was detected by enhanced chemiluminescence
(6)
Dopaminergic neurons are vulnerable to dysregulation of YEATS2-dependent calcium homeostasis
iScience
June 19, 2026
Luca Lo Piccolo, Ranchana Yeewa, Pitiporn Noisagul, Arnaud Monteil, Vorasuk Shotelersuk, Salinee Jantrapirom
AB_221569 Tyrosine hydroxylase (TH),
Merck
,
AB152
; RRID: AB_390204 Anti-mouse IgG (H + L)-HRP Conjugate,
Biorad
,Cat#
1706516
; RRID: AB_2921252 Anti-rabbit IgG (H + L)-HRP Conjugate,
Biorad
,Cat#
1706515
; RRID: AB_11125142
Goat anti-rabbit IgG
(7)
Estradiol-17β, a key embryonic signal, regulates gene expression and cellular functions in the porcine trophoblast†
Biology of Reproduction
June 15, 2026
Ewelina Goryszewska-Szczurek, Agnieszka Waclawik
polyclonal",
1:2000
Anti-rabbitHRP,Immun-Star
Goat
AntiRabbit (GAR)-HRP Conjugate,"
Bio-Rad Laboratories
,
1705046
","
Goat
, polyclonal",
1:20 000
List of antibodies
used in Western blot
experiments
(8)
TPI and GAPDH Interact with Rad9, Linking Glycolytic Enzymes to Cancer
Int J Mol Sci
June 12, 2026
Vivienne X. Y. Chua, Joyce M. X. Yip, Melody T. K. Cho, Sumi Z. Q. Lin, Rich Tan, Donna G. K. Lee, Kexin Dai, Teck K. Lim, Qingsong Lin, Rachel Lehming-Teo,
et al.
Life technologies
A6428
, Carlsbad, CA, USA), Goat anti-
mouse
-
horseradish
peroxidase (anti-mouse-HRP,
BioRad
1706516
, Hercules, CA, USA);
Yeast
Media:
Adenine
(
Sigma
A9126
),
Yeast Extract
(
Bio Basic
G0961), Peptone (
BD
(9)
Selective targeting of a histone-like silencer Sfx to the R6K conjugal transfer operon
Nucleic Acids Res
June 10, 2026
Bing Wang, Ritika Gupta, Nathan Blaine, Barbare Khitiri, Catherine Jordan, Natalia Molotievskiy, David Dunlap, Laura Finzi, Irina Artsimovitch
incubated with secondary antibody [
1:10 000
;
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
(
Bio-Rad
, cat#
1706515
)] in blocking buffer for 1 h at room temperature. The
membrane
was then washed five times with TBST.
(10)
Transsynaptic complex dysfunction in the hippocampus of Alzheimer's disease patients
Front Aging Neurosci
June 10, 2026
Ashly Hindle, Yong Chen, Xiangling Yin, Maria Manczak, Boris Decourt, Volker Neugebauer
washed 3 times with TBST and incubated with anti-rabbit peroxidaseconjugated secondary antibody (cat#
1706515
,
Bio-Rad Laboratories
, Hercules, CA, USA) for 1 h at room temperature. Proteins were detected using
(11)
Heat-processed rodent chow alters nutritive content and improves metabolic outcomes in insulin-resistant female mice
Front Nutr
June 8, 2026
Adelaide E. Weidner, Kenji Vann, Denise Ivey, Zachary R. Sechrist, Calvin L. Cole, Olga Astapova
milk/TBST) according to concentrations in Table 1. Blots were incubated in secondary antibody (
Bio-Rad
#
1706515
) diluted
1:5,000
in blocking buffer for 1 h at room temperature and imaged with ECL substrate (
Bio-Rad
(12)
Metformin Redirects Autophagy from Bulk Turnover to Mitochondrial Clearance
bioRxiv
June 6, 2026
Thuraya M. Mutawi, Shweta R. Malvankar, Andrea Graziani, Udita Shah, Khanh Nguyen, David G. Broadbent, Zachary Clark, Michaella J. Rekowski, Susan M. Lunte, Carlo Barnaba
14793), anti-PEN2 (Cat. # 8502). HRP-conjugated secondary antibodies (
goat anti-rabbit IgG
, Cat. #
1706515
;
goat anti-mouse IgG
, Cat. #
1706516
), as well as anti-
WIPI2
(
clone
2A2, Cat. #
MCA5780GA
), were from
(13)
Targeted lipidomics between hPheo1 and SDHB KD cells reveal changes in bioactive lipids and PKC with polyamine pathway inhibition
Physiol Rep
June 5, 2026
Abdel A. Alli, Niharika Bala, Yiling Xu, Nancy D. Denslow, Hans K. Ghayee
series of three washes in 1XTBS before being incubated in
goat
anti‐rabbit secondary antibody (Cat. No.
1706515
) (
BioRad
) diluted
1:3000
(prepared in blocking solution) at room temperature while rocking for 1 h.
(14)
Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia
J Cell Biol
June 1, 2026
Ziyan Wang, Bradley M. Bartholomai, Bin Wang, David Ritz, Daniel Schultz, Jennifer J. Loros, Jay C. Dunlap
DM1A,
1:5000
Anti-V5 (
mouse
),Invitrogen #46–0705,
1:5000
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
,
BIO-RAD
#
1706515
,
1:5000
Goat Anti-Mouse IgG (H + L)-HRP Conjugate
,
BIO-RAD
#
1706516
,
1:5000
(15)
Variants in glycine decarboxylase activate catabolic mechanisms of mitochondrial energy metabolism in the brain
Journal of Biological Chemistry
June 1, 2026
Alejandro Lopez-Ramirez, Andrew J. Worth, Ziyi (Lindsay) Wang, Saliha Yilmaz, Sebastian Hayes, Hsin-Yao Tang, Joseph Farris, Md Suhail Alam, Prasad Padmanabhan, Kasturi Haldar
anti-SHMT2 (rabbit,
Proteintech
,
11099-1-AP
,
1:800
). Secondary antibodies were
goat anti-mouse IgG
(
Bio-Rad
,
1706516
,
1:5000
) and
goat anti-rabbit IgG
(
Bio-Rad
,
1706515
,
1:5000
). Statistical analysis Statistical analysis
(16)
Phospho-mimic βIII-tubulin rescues microtubule and cardiac defects in Duchenne muscular dystrophy mice
Journal of Molecular and Cellular Cardiology
June 1, 2026
Delong Zhou, Julie Nouet, Elam Mesa, Vasisht Yegneshwaran, Geovanni Geukgeuzian, Ifeanyichukwu D. Adibemma, Edwin Ramirez, Nehal Kamal Ali, Sabine Hilfiker, Hong Li,
et al.
vinculin (
Sigma
V9131
,
1:2,000
,
mouse
). The secondary antibodies include
goat
anti-rabbit HRP (
Bio-Rad
1705046
,
1:10,000
) and goat anti-mouse HRP (
Bio-Rad
1705047
,
1:10,000
). Ventricular
Cardiomyocyte
Isolation
Cardiomyocytes
(17)
Ulmus pumila Linné (Ulmi) Extract Attenuates Inflammatory Responses in Atopic Dermatitis by Modulating Lipid Peroxidation and Oxidative Stress
Antioxidants (Basel)
May 29, 2026
Min Jung Kim, Mi Jin Jang, Young Zoo You, Ye Jin Yang, Ji Woong Heo, Hee Ho Kim, Hun Hwan Kim, Se Hyo Jeong, Gon Sup Kim, Young Woo Kim,
et al.
with TBS-T,
membranes
were incubated with HRP-conjugated secondary antibodies (
goat anti-mouse IgG
, #
1706516
;
goat anti-rabbit IgG
, #
1706515
;
Bio-Rad
, Hercules, CA, USA) for 2 h at room temperature. Protein bands
(18)
Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expression
Nucleic Acids Res
May 26, 2026
Zhiyun Yang, Zongchao Jia
temperature for 1 h with shaking. The secondary antibodies used were
goat
anti-rabbit HRP (
Bio-Rad
, #
1706515
), goat anti-mouse HRP (
Bio-Rad
, #
1721011
). Followed by washing the
membrane
three times with TBST at
(19)
Fatty acid‑activated proton transporter SR4 prevents hepatic steatosis and metabolic alterations in diabetic mice by improving mitochondria function, energy balance and oxidative stress
Experimental and Therapeutic Medicine
May 20, 2026
James Figarola, Jyotsana Singhal, Sharad Singhal
1:3,000
dilution of peroxidase-labeled secondary antibodies (
goat anti-rabbit IgG HRP
conjugate; cat. no.
1706515
;
Bio-Rad Laboratories
, Inc.) at room temperature for 2 h, and an ECL system (Western Lightning® Chemiluminescence
(20)
Vespakinin-M delineates an AMPK/mTOR-arginine-TCA cycle axis to act as an immunometabolic switch in post-stroke microglia
Redox Biol
May 12, 2026
Dexiao Wang, Jingyu Zhang, Zhejun Zhuang, Qian Wang, Jie Li, Xue Wang, Kunkun Li, Yunyun Liu, Yanhui Cao, Lijuan Li,
et al.
10-min washes with TBST,
membranes
were incubated with HRP-conjugated
goat anti-rabbit IgG
(
1:3000
, #
1706515
,
Bio-Rad
, USA) for 1 h at RT. Protein bands were visualized using enhanced chemiluminescence substrate
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Reviews
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(1)
9/13/2022
Reliable Secondary Antibody with Clear Detection
Arianna Napoli
I used Goat Anti-Mouse IgG (H + L)-HRP Conjugate as secondary antibody conjugate to detect two mouse IgG in Western blots. This time for the dilution I used 1:5000 in Non-Fat Dry Milk (NFDM), but I ...
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HRP and AP Conjugates from Bio-Rad
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