Fig 1: Effect of PROKR antagonists on the permeability of the BBB co-culture model. Measurement of the permeability of the co-culture model was assessed in the presence or absence of PROK1 (30 nM) (A) or PROK2 (60 nM) (B), pretreated with PROKR antagonists, PC7 or PKR-A (1 µM). Data are presented as a percentage of permeability coefficient (Pe) compared to untreated insert, as mean ± SEM, from three independent experiments. Statistical analysis: unpaired two-tailed t-test was used. Statistical significance is indicated as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, compared to CTL, and ## p ≤ 0.01, ### p ≤ 0.001 compared to PROK1 or PROK2 treatments when used alone.
Fig 2: Effect of PROK1 and PROK2 on the migration of b.End.3, C8-D30, and N2a cell lines. Effect of PROK1 on the migration of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the migration of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 compared to the condition without prokineticin.
Fig 3: Effect of PROKs on the permeability of the BBB model. Permeability assessment of the co-culture of bEnd.3/C8-D30 cells 24 h after treatment with PROK1 (A) or with PROK2 (B). Data are expressed as a percentage of permeability coefficient (Pe) (as mean ± SEM) compared to untreated control. Statistical analysis: unpaired two-tailed t-test was used from three independent experiments. Statistical significance is indicated as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, compared to the condition without prokineticin.
Fig 4: Effect of PROK1 and PROK2 on the proliferation of b.End3, C8-D30, and N2a cell lines. Effect of PROK1 on the proliferation of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the proliferation of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, compared to the condition without prokineticin.
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