EnGenMutation Detection Kit E3321S from New England Biolabs

Product Specs
ItemEnGenMutation Detection Kit
CompanyNew England Biolabs
Price $225.00 Inquire
Catalog NumberE3321S
Size25 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Neurotrophic Modulation Restores Motor and Developmental Defects in Zebrafish Models of ints11 DeficiencyJ NeurochemMarch 16, 2026Anna Pistocchi, Elena Chiricozzi, Matilde Molteni, Gaia Galassi, Laura Mauri, Francesca Balistreri, Stefania Magri, Anna Marozzi, Franco Taroni, Alex PezzottaT7 endonuclease I (T7EI) with the EnGen Mutation Detection Kit (New England Biolabs, Ipswich, UK; cat.no E3321S). Reaction products were resolved on 2% agarose gel to detect digested PCR fragments, indicating the

(2) Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO platforms for cell line developmentBiotechnology ProgressFebruary 22, 2026Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford(T7EI) assay Genome editing efficiency was assessed using the EnGen® Mutation Detection Kit (NEB, Cat# E3321S). After DNA extraction, 100 ng of genomic DNA was used to perform target specific PCR, the corresponding

(3) Evaluation of gene editing in CHO cells using the Cas‐ CLOVER systemBiotechnology ProgressFebruary 8, 2026Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, et al.manufacturer's protocol. The mismatch assay was performed using the EnGen Mutation Detection Kit (Cat# E3321S, NEB) according to the manufacturer's instructions. Briefly, a region flanking GS exon 5 and the potential

(4) Manipulation of Alternative Splicing of IKZF1 Elicits Distinct Gene Regulatory Responses in T CellsCellsJanuary 24, 2026Lucia Pastor, Jeremy R. B. Newman, Colin M. Callahan, Rebecca R. Pickin, Mark A. Atkinson, Suna Onengut-Gumuscu, Patrick Concannondays post-transfection (New England Biolabs, Ipswich, MA, USA, EnGen® Mutation Detection Kit, Cat# E3321S). Cloned Jurkat cell lines were isolated by serial dilution in U-bottom 96-well plates and cultured

(5) Heat shock induces alternative polyadenylation through dynamic DNA methylation and chromatin loopingCell stress & chaperonesJuly 1, 2025Fink EE, Zhang Y, Santo B, Siddavatam A, Ou R, Nanavaty V, Lee BH, Ting AH.editing efficiency of the pooled cells was determined using the T7 endonuclease I (T7EI) assay (NEB #E3321S). Briefly, genomic DNA from transfected cells was amplified using primers flanking the sgRNA-targeted

(6) Neuron–non‐neuron electrical coupling networks are involved in chronic stress‐induced electrophysiological changes in lateral habenular neuronsJ PhysiolApril 1, 2025Kenji Yamaoka, Kanako Nozaki, Meina Zhu, Haruhi Terai, Kenta Kobayashi, Hikaru Ito, Miho Matsumata, Hidenori Takemoto, Shinya Ikeda, Yusuke Sotomaru, et al.the PCR products were denatured, digested at 37°C for 15 min with an EnGen Mutation Detection Kit (E3321S; New England Biolabs, Ipswich, MA, USA), and analysed by electrophoresis in 2% agarose gels stained

(7) Neuron–non‐neuron electrical coupling networks are involved in chronic stress‐induced electrophysiological changes in lateral habenular neuronsThe Journal of PhysiologyApril 1, 2025Kenji Yamaoka, Kanako Nozaki, Meina Zhu, Haruhi Terai, Kenta Kobayashi, Hikaru Ito, Miho Matsumata, Hidenori Takemoto, Shinya Ikeda, Yusuke Sotomaru, et al.the PCR products were denatured, digested at 37°C for 15 min with an EnGen Mutation Detection Kit (E3321S; New England Biolabs, Ipswich, MA, USA), and analysed by electrophoresis in 2% agarose gels stained

(8) SLMAP3 is crucial for organogenesis through mechanisms involving primary cilia formationOpen biologyOctober 1, 2024Dias AP, Rehmani T, Applin BD, Salih M, Tuana B.was confirmed with T7 endonuclease-based EnGen Mutation Detection Kit (New England Biolabs, cat. no. E3321S). For FGFR1OP2-sgRNA#2, we used the following pair of primers: 5′-GATGGCCTGCTCTAGACACT and 5′-TGGCGGTGAAGGATGTCTTA;

(9) SH-SY5Y human neuronal cells with mutations of the CDKN2B-AS1 gene are vulnerable under cultured conditionsBiochemistry and Biophysics ReportsJuly 1, 2024Michiko Ohno-Oishi, Zou Meiai, Kota Sato, Seiya Kanno, Chihiro Kawano, Makoto Ishikawa, Toru Nakazawaaccording to the manufacturer's protocol with the EnGen Mutation Detection Kit (New England Biolabs, E3321S). The density of the amplicon and digested band was determined with software (Image Lab 3.0; Bio-Rad). 2.9

(10) CRISPR-Cas9-mediated deletion of carbonic anhydrase 2 in the ciliary body to treat glaucomaCell Reports MedicineMay 21, 2024Jiaxuan Jiang, Kangjie Kong, Xiuli Fang, Deming Wang, Yinhang Zhang, Peiyuan Wang, Zefeng Yang, Yuwei Zhang, Xiaoyi Liu, Tin Aung, et al.mutations. Finally, the sample was digested with the EnGen Mutation Detection Kit (New England BioLabs, E3321S) for 15 min at 37°C followed by Proteinase K digestion for 5 min at 37°C, and the resulting products

(11) Enhancer switching in cell lineage priming is linked to eRNA, Brg1’s AT-hook, and SWI/SNF recruitmentMolecular CellMay 16, 2024Dhurjhoti Saha, Srinivas Animireddy, Junwoo Lee, Anna Thommen, McKenzie M. Murvin, Yue Lu, J. Mauro Calabrese, Blaine Bartholomewassays MycoAlert,Lonza,cat#LT07–218 QuickExtract DNA extraction kit,Lucigen,cat#QE09050 EnGen Mutation detection kit,NEB,cat#E3321S Alkaline staining kit,SBI,cat#AP100B-1 Qubit dsDNA HS kit,Thermo Fisher Scientific,cat#Q32581 NEB

(12) Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24iScienceJuly 21, 2023Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.T7E1 assays were performed using the EnGen® Mutation Detection Kit per manufacturers protocol (NEB E3321S) and gene specific primers to amplify a ∼500bp region containing PAM site targeted by Synthego to perform

(13) Membrane-Tethered Mucin 1 Is Stimulated by Interferon and Virus Infection in Multiple Cell Types and Inhibits Influenza A Virus Infection in Human Airway EpitheliummBioAugust 30, 2022Iverson E, Griswold K, Song D, Gagliardi TB, Hamidzadeh K, Kesimer M, Sinha S, Perry M, Duncan GA, Scull MA.Sorted transduced cells were frozen for later use or analyzed with an EnGen mutation detection kit (no. E3321S, New England BioLabs) for on-target gene editing confirmation (forward primer, 5′-AGC ACT TCT CCC CAG

(14) Flow Assisted Mutation Enrichment (FAME): A highly efficacious and efficient method to enrich Double Knockouts (DKO) after gene editingPLOS ONEMarch 4, 2021Michael Hansen, Xiaopin Cai, Sara Bowen, David A. Largaespada, Ming V. Li(T7EI) assay The T7EI assay was conducted with kits purchased from New England Biolabs (Catalog # E3321S), according to manufacturer’s instruction. Briefly, PCR was conducted with provided high-fidelity polymerase

(15) Development of a CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brainScience advancesMarch 1, 2020Sun H, Fu S, Cui S, Yin X, Sun X, Qi X, Cui K, Wang J, Ma L, Liu FY, et al.sequence was amplified with polymerase chain reaction (PCR) by Q5 Hot Start High-Fidelity 2× Master Mix (E3321S, New England Biolabs) with the following program: preheat at 98°C for 30 s, 35 cycles of three-step

(16) Niche stiffness underlies the ageing of central nervous system progenitor cellsNatureSeptember 5, 2019Michael Segel et al.28104) and the surveyor assay was performed as directed from the Engen Mutation Detection Kit (NEB; E3321S). Samples were subsequently run on a 2% agarose gel and band intensity was measured using ImageJ. For

(17) Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cellsCommunications biologyJanuary 1, 2018Kogata N, Bland P, Tsang M, Oliemuller E, Lowe A, Howard BA.QuickExtract DNA Extraction Solution (QE0905T, Qiagen, Peterborough, UK) and EnGen™ Mutation Detection Kit (E3321S, New England Biolabs, Herts, UK) according to the manufacturer’s instructions. We tested four different

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12/2/2022Good Kit for Measuring Editing Efficiency Dimi ComfuWe were interested in studying the role of DNA methylation in the differentiation of of CD4 T-cells. CRISPR-Cas9 approaches were used to knock-out epigenetic enzymes in primary cells and cell lines. ... Read Review

EnGenMutation Detection Kit

EnGenMutation Detection Kit from New England Biolabs

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New England Biolabs

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EnGenMutation Detection Kit from New England Biolabs

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