Fig 1: Administration of 70 mg/kg Ax up-regulates GCase expression in penumbra after dMCAO. (A) Representative immunostaining images of Nestin and GCase in penumbra post-dMCAO on day 7 in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg. DAPI was used to counterstain the nuclei. Insets were magnified images from each photograph at high magnification. Scale bar: 100 μm. (B) Semi-quantitative analysis of the expression of GCase from (A). ***P < 0.001 vs. dMCAO + Vehicle group. (C) Immunoblot bands showing the expression level of GCase in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg, respectively. GAPDH was served as an internal control. (D) Semi-quantitative analysis of GCase expression from (C). ***P < 0.001 vs. dMCAO + Vehicle group. (E) Representative immunostaining images of DCX (red) and GCase (green) in penumbra post-dMCAO on day 7 in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg. DAPI was utilized to counterstain the nuclei. Insets were magnified images from each photograph at high magnification. Scale bar: 20 μm. (F) Representative immunostaining images of GCase (red) and MAP2 (green) in penumbra post-dMCAO on day 7 in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg. DAPI was used to counterstain the nuclei. Insets were magnified images from each photograph at high magnification. Scale bar: 20 μm. Ax, ambroxol; dMCAO, distal middle cerebral artery occlusion; GCase, glucocerebrosidase; DCX, doublecortin.
Fig 2: Ax increases the expression of GCase and β-catenin when NSCs exposed to OGD. (A–C) Statistical analysis of GCase (A), β-catenin (B) and Axin2 (C) mRNA expression using RT-qPCR assays in different groups. *P < 0.05, **P < 0.01 vs. control group; #P < 0.05 vs. OGD + Vehicle; @P < 0.05 vs. OGD + Ax-30 μM. (D) Bands depicted the expression of GCase, β-catenin, p-β-catenin and Axin2 in different groups. GAPDH was used as an internal control. (E–H) Semi-quantitative analysis of β-catenin (E), p-β-catenin (F), GCase (G) and Axin2 (H) expression from (D). *P < 0.05, **P < 0.01 vs. control group; #P < 0.05 vs. OGD + Vehicle; @P < 0.05 vs. OGD + Ax-30 μM. (I) Representative immunostaining images of GCase (green), β-catenin (red) and Nestin (white) in various groups. Scale bar: 20 μm. Ax, ambroxol; OGD, oxygen glucose deprivation.
Supplier Page from Abcam for Recombinant Mouse GBA protein (Tagged)