Fig 1: NMN supplementation ameliorates cell death of chondrocytes with mtRC dysfunction.Chondrocytes from 1-month-old Cre and CreTW mice were cultured for 3 days in −aa medium in the absence (−) or presence of rapamycin or increasing concentrations of NMN. (A) Cell morphology was studied by microscopy and (B) cell death by flow cytometry. (C) The proportion of AnxA5-Cy2+/SytoxBlue− and AnxA5-Cy2+/SytoxBlue+ dying cells is given (n = 3 liters per group). Quantitative data are means ± SEM. One-way ANOVA with Bonferroni’s multiple comparisons test, **P < 0.01. Scale bar, 50 μm.