Mouse ICAM-1 / CD54 Protein (His Tag) 50440-M08H from Sino Biological, Inc.

Product Specs
ItemMouse ICAM-1 / CD54 Protein (His Tag)
CompanySino Biological, Inc.
Price $1,398.00Supplier Page View Company Product Page
Catalog Number50440-M08H
ActivityMeasured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 µg/mL, 100 µL/well), approximately > 40% cells will adhere specifically
FormatLyophilized from sterile PBS, pH 7.4
Size500 µg
NCBI Gene AliasesCD54, Icam-1, Ly-47, MALA-2
NCBI Full Gene Nameintercellular adhesion molecule 1
Source/Expression SystemHEK293 Cells
Molecule NameICAM1
SpeciesMouse
Molecular WeightThe recombinant mouse ICAM1 consists of 469 amino acids after removal of the signal peptide. and has a predicted molecular mass of 51.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1 is approximately 80-90 kDa due to glycosylation
TypeRecombinant protein
Purity≥ 96 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC
Conjugate/TagC-His

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) The tropomyosin 3.1/3.2 inhibitor ATM-3507 alters B-cell actin dynamics and impairs the growth and motility of diffuse large B-cell lymphoma cell linesFrontiers in ImmunologyNovember 5, 2025Abhishek Bedi, Kate Choi, Alyssa Iskierski, Michael R. Goldor 0.625 μg/cm2 goat anti-mouse IgG plus 0.15 µg/cm2 ICAM-1 (Sino Biological Inc., Beijing, China #50440-M08H) for 30 min at 37°C. Afterwards, the coverslips were blocked for 30 min at room temperature with PBS

(2) Microdevice for confinement of T-cells on functionalized bio-interfaces†Lab on a ChipMay 28, 2025Christoph Trenzinger, Caroline Kopittke, Barbora Kalousková, Nemanja Šikanić, Marina Bishara, Gerhard J. Schütz, Mario Brameshuber(nickel salt) (Ni-NTA-DGS, 790404P, Avanti Polar Lipids). B7-1 (50446-M08H, Sino Biological), ICAM-1 (50440-M08H, Sino Biological), and MMC-peptide loaded I-Ek (pMHC, prepared and labelled as described previously27)

(3) Functional differences between rodent and human PD-1 linked to evolutionary divergenceScience immunologyJanuary 3, 2025Masubuchi T, Chen L, Marcel N, Wen GA, Caron C, Zhang J, Zhao Y, Morris GP, Chen X, Hedrick SM, et al.(#10084-H08H), moPD-1-His (#50124-M08H), moPDL1-His (#50010-M08H), huICAM-1 (#10346-H08H), and moICAM-1 (#50440-M08H) were purchased from SinoBiological. Recombinant protein huPD-1ECD-His (#PD1-H5221), huPDL1ECD-huFc

(4) Direct recognition of an intact foreign protein by an αβ T cell receptorNature communicationsOctober 11, 2024Almeida CF, Gully BS, Jones CM, Kedzierski L, Gunasinghe SD, Rice MT, Berry R, Gherardin NA, Nguyen TT, Mok YF, et al.

(5) Ligand-induced segregation from large cell-surface phosphatases is a critical step in γδ TCR triggeringCell reportsSeptember 24, 2024Li F, Roy S, Niculcea J, Gould K, Adams EJ, van der Merwe PA, Choudhuri K.monomer-biotin,NIH tetramer core,N/A pOVA-I-Ab tetramer,NIH tetramer core,N/A ICAM-1-His,Sino Biological,Cat#: 50440-M08H Nck SH3.1,Peptide 2.0,Batch #: 110165-001 Experimental models: Cell lines,Experimental models: Cell

(6) Ectopic Expression of a Truncated Isoform of Hair Keratin 81 in Breast Cancer Alters Biophysical Characteristics to Promote Metastatic PropensityAdvanced ScienceFebruary 1, 2024Diane S. Kang, Aidan Moriarty, Yiru Jess Wang, Amal Thomas, Jia Hao, Bret A. Unger, Remi Klotz, Shamim Ahmmed, Yonatan Amzaleg, Stuart Martin, et al.with 1% BSA for 1 h and a solution of 10 µg mL−1 recombinant mouse ICAM‐1 with poly‐histidine tag(Cat. 50440‐M08H, SinoBiological) was injected to the supported lipid bilayer, incubated at RT for 40 min and tethered

(7) WAVE2 Regulates Actin-Dependent Processes Induced by the B Cell Antigen Receptor and IntegrinsCellsNovember 25, 2023Bedi A, Choi K, Keane C, Bolger-Munro M, Ambrose AR, Gold MR.Jackson ImmunoResearch, West Grove, PA, USA #115-005-020), 0.15 µg/cm2 ICAM-1 (Sino Biological Inc., Beijing, China #50440-M08H), 0.625 μg/cm2 goat anti-mouse IgG plus 0.15 µg/cm2 ICAM-1, or 2.4 μg/cm2 fibronectin (FN; Sigma Aldrich

(8) cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8(+) T cell function and anti-tumor immunityImmunityJune 13, 2023Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, Bui JD, Hui E.423113 Zombie Aqua,BioLegend,Cat # 423102 Fibronectin,Corning,Cat # 354008 Mouse ICAM1,Sino Biological,Cat # 50440-M08H BV421 OVA257–264 H-2Kb tetramer,NIH Tetramer Core Facility,N/A Tyramide-488 Reagent,ThermoFisher Scientific,Cat

(9) Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockadeMed (New York, N.Y.)October 14, 2022Palmer DC, Webber BR, Patel Y, Johnson MJ, Kariya CM, Lahr WS, Parkhurst MR, Gartner JJ, Prickett TD, Lowery FJ, et al.High Output reagents,Illumina,20024907 histidine tagged CD80,in house,N/A ICAM-1,Sino Biological,50440-M08H-20 Critical commercial assays,N/A,N/A Deposited data,, 10x Genomics scRNA matrices,This paper,https://github.com/CBIIT-CGBB/CISH_Cell_Med Experimental models

(10) A B-cell actomyosin arc network couples integrin co-stimulation to mechanical force-dependent immune synapse formationeLifeApril 11, 2022Wang JC, Yim YI, Wu X, Jaumouille V, Cameron A, Waterman CM, Kehrl JH, Hammer JA.protein",Streptavidin,MilliporeSigma,Cat# 189730, "peptide, recombinant protein",Mouse histidine-tagged ICAM-1,Sino Biological,Cat# 50440-M08H, Commercial assay or kit,Nucleofector Kit V,Lonza,Cat# VCA-1003, Commercial assay or kit,In-Fusion

(11) Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact ProteinsACS nanoSeptember 28, 2021Hellmeier J, Platzer R, Mühlgrabner V, Schneider MC, Kurz E, Schütz GJ, Huppa JB, Sevcsik E.and SLBs were formed as described above. Upon incubation of DNA origami on SLBs, His10-tag ICAM-1 (50440-M08H, Sino Biological) (270 ng mL–1) and His10-tag B7-1 (50446-M08H, Sino Biological) (130 ng mL–1) were

(12) DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigensProceedings of the National Academy of Sciences of the United States of AmericaJanuary 26, 2021Hellmeier J, Platzer R, Eklund AS, Schlichthaerle T, Karner A, Motsch V, Schneider MC, Kurz E, Bamieh V, Brameshuber M, et al.

(13) Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFRMolecular and cellular neurosciencesOctober 1, 2020Chen ZH, Luo XC, Yu CR, Huang L.

(14) PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2The Journal of cell biologyJune 1, 2020Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma Y, Wang H, et al.2 D, PLB was functionalized by a mixture of 5 nM pMHC-I-His, 2 nM mouse ICAM-His (Sino Biological; #50440-M08H), 3 nM mouse CD86-His (Sino Biological; #50068-M08H), and either 8 nM mouse PD-L1–His (Sino Biological;

(15) PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysImmunityDecember 17, 2019Zhao Y, Lee CK, Lin CH, Gassen RB, Xu X, Huang Z, Xiao C, Bonorino C, Lu LF, Bui JD, et al.# 50446-M08H Mouse CD86-His,Sino Biological,Cat # 50068-M08H Mouse ICAM-His,Sino Biological,Cat # 50440-M08H SIINFEKL peptide,Anaspec,Cat# AS-60193–1 Mouse IL-2,Thermo Fisher Scientific,Cat# 14802164 Protein

(16) Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T CellsCell reportsJuly 10, 2018Zhao Y, Harrison DL, Song Y, Ji J, Huang J, Hui E.50010-m08h Mouse B7.2EX-His,Sino Biological,Cat # 50068-m08h Mouse ICAMEX-His,Sino Biological,Cat # 50440-m08h SIINFEKL peptide,Anaspec,Cat # AS-60193-1 Live/Dead Aqua,Thermo Fisher Scientific,Cat # L34966 Critical

(17) Supported Lipid Bilayer Technology for the Study of Cellular InterfacesCurrent protocols in cell biologySeptember 1, 2015Crites TJ, Maddox M, Padhan K, Muller J, Eigsti C, Varma R.1) Mono-biotinylated α-CD3 (Fleire and Batista, 2009) Streptavidin Poly-His-tagged ICAM-1 (Sino Biological, cat. no. 50440-M08H-20) Casein solution (See Support Protocol 3) HBS-BSA (see recipe) Additional reagents and equipment

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Mouse ICAM-1 / CD54 Protein (His Tag)

Mouse ICAM-1 / CD54 Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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Mouse ICAM-1 / CD54 Protein (His Tag) from Sino Biological, Inc.

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