Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) 10108-H02H from Sino Biological, Inc.

Product Specs
ItemHuman ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag)
CompanySino Biological, Inc.
Price $218.00Supplier Page View Company Product Page
Catalog Number10108-H02H
Size20 µg
Conjugate/TagC-human IgG1-Fc
Purity≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 kDa band in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameACE2
Source/Expression SystemHEK293 Cells
NCBI Full Gene Nameangiotensin converting enzyme 2
NCBI Gene AliasesACEH
FormatLyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Activity1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (2019-nCoV) Spike S1-His (Cat: 40591-V08B1) at 2 µg/mL (100 µL/well) can bind human ACE2 (hFc tag) (Cat: 10108-H02H), the EC50 of ACE2 is 5-35 ng/mL. 2.Captured ACE2（10108-H02H on proA Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (Cat.No.40591-V08H41) with an affinity constant of 11.78 nM as determined in a SPR assay (Biacore T200)(Routinely tested). 3.Captured ACE2（10108-H02H on anti-Human IgG Fc via CM5 Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (Cat. No. 40592-V08H121) with an affinity constant of 5.667 nM as determined in a SPR assay (Biacore T200)(Routinely tested). 4.Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein, hFc Tag (Cat. No. 10108-H02H) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) The pan-variant potential of light: 425 nm light inactivates SARS-CoV-2 variants of concern and non-cytotoxic doses reduce viral titers in human airway epithelial cellsmSphereJune 25, 2025Nathan Stasko, Leslee Arwood, Nicole Jandick, Derry Spragion, Rachel C. Roberts, Mónica Setién, Ibrahim Henson, Abigail Annas, M. Leslie Fulcher, Marisa Brotton, et al.assay High-binding ELISA 96-well plates were coated with 200 ng of recombinant human ACE-2 protein (Sino Biological #10108-H02H) or human DPP-4 (Sino Biological #10688-H01H) in 100 µL PBS and were incubated at 4°C overnight. Wells

(2) Rational Development of a Novel Emulgel Adjuvant for Single‐Shot Effective Vaccination: A Multivariate Analysis ApproachAdvanced MaterialsJune 3, 2025Ahmed O. Shalash, Waleed M. Hussein, Ummey J. Nahar, Jingwen Wang, Lantian Lu, Armira Azuar, Jolynn J. E. Kiong, Prashamsa Koirala, Zeinab G. Khalil, Istvan Tothobtained from Invivogen (San Diego, USA). RBD was obtained from Sino Biologicals (Beijing, China, Cat. No. 10108‐H02H). AlphaLisa kits for IFN‐γ and IL‐4 interleukins analyses (AL501C and AL567HV, respectively) and opaque

(3) Altered hACE2 binding affinity and S1/S2 cleavage efficiency of SARS-CoV-2 spike protein mutants affect viral cell entryVirologica SinicaAugust 1, 2023Wang K, Pan Y, Wang D, Yuan Y, Li M, Chen Y, Bi L, Zhang XE.(0.2 mol/L, pH=8.0) as Running buffer. Protein A chip (Cytiva) was used and ACE2-hFc (SinoBiological, 10108-H02H) at a concentration of 10 μg/mL as a ligand was captured to ∼150 RU on the chip surface through Fc tag.

(4) Multiplexed on-yeast serological assay for immune escape screening of SARS-CoV-2 variantsiScienceMay 19, 2023Lopez-Morales J, Vanella R, Utzinger T, Schittny V, Hirsiger J, Osthoff M, Berger CT, Guri Y, Nash MA.Bovine serum albumin,Sigma-Aldrich,Cat# 10735086001 Human recombinant ACE2 Protein (hACE2-hFc Tag),SinoBiological Europe GmbH,Cat# 10108-H02H ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, SARS-CoV-2

(5) ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathwayJournal of translational medicineFebruary 9, 2023Chen F, Chen Y, Ke Q, Wang Y, Gong Z, Chen X, Cai Y, Li S, Sun Y, Peng X, et al.The recombinant RBD (SinoBiological, 40592-V05H) and recombinant human ACE2 proteins (SinoBiological, 10108-H02H) were captured with an anti-mIgG Fc Capture (AMC) probe in PBST solution and then incubated with recombinant human ApoE2

(6) Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolutionNatureFebruary 1, 2023Cao Y, Jian F, Wang J, Yu Y, Song W, Yisimayi A, Wang J, An R, Chen X, Zhang N, et al.hACE2 against the pseudoviruses were determined with the same procedure, using the hACE2-Fc dimer (10108-H02H, Sino Biological), and each experiment was conducted in five biologically independent replicates. DMEM

(7) Effect of acetic acid inactivation of SARS-CoV-2PloS oneJanuary 1, 2023Amruta N, Maness NJ, Gressett TE, Tsuchiya Y, Kishi M, Bix G.assay buffer (10-fold dilution was achieved using assay buffer containing hACE2–Fc (0.5 μg/mL, Cat# 10108-H02H, Sino Biological) and incubated at 37°C for 30 minutes. The plates were washed and incubated with horseradish

(8) A potent, broadly protective vaccine against SARS-CoV-2 variants of concernNPJ vaccinesNovember 12, 2022Wang Z, An J, Liu K, Yu P, Fang X, Li J, Zhu H, Zhu Q, Huang C, Zhang C, et al.affinity of modified S-trimer to human ACE2. In general, the Fc-tagged human ACE2 (Sino Biological, Cat.10108-H02H) was immobilized to human Fc (HFC) Probes (Gator Bio, Cat.160003) at 10 μg/mL. The variant S-trimer

(9) Development of a thermostable SARS-CoV-2 variant-based bivalent protein vaccine with cross-neutralizing potency against Omicron subvariantsVirologyNovember 1, 2022Wang R, Huang X, Cao T, Sun C, Luo D, Qiu H, Wu M, Huang X, Yu C, Li J, et al.SCTV01C antigen to human ACE2 receptor were determined on OCTET RED96e (ForteBio). hACE2-Fc (10 μg/mL) (10108-H02H, Sino Biological) was immobilized on ProA Sensor. After baseline, SCTV01C antigens were serially diluted

(10) LRRC15 inhibits SARS-CoV-2 cellular entry in transPLoS biologyOctober 1, 2022Song J, Chow RD, Peña-Hernández MA, Zhang L, Loeb SA, So EY, Liang OD, Ren P, Chen S, Wilen CB, et al.#14025–092) and 2% FBS (VWR, #104B16). The plates were then incubated with either ACE2-Fc (Sino Biological, #10108-H02H, starting concentration, 10 μg/mL), LRRC15-Fc (Sino Biological, #15786-H02H, 100 μg/mL) or human IgG1

(11) Utilization of Receptor-Binding Domain of SARS-CoV-2 Spike Protein Expressed in Escherichia coli for the Development of Neutralizing Antibody AssayMolecular BiotechnologySeptember 14, 2022Termsak Tantiwiwat, Apisitt Thaiprayoon, Ake-kavitch Siriatcharanon, Chakrit Tachaapaikoon, Nongluk Plongthongkum, Dujduan Waraho-Zhmayev(Cat. No. 40592-V08H90); and Omicron (B.1.1.529) variant (Cat. No. 40592-V08H121). ACE2-hFc (Cat. No. 10108-H02H) was also purchased from Sino Biological. The % inhibition was calculated using this equation: Inhibition

(12) APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patientsSignal transduction and targeted therapyAugust 1, 2022Zhang H, Shao L, Lin Z, Long QX, Yuan H, Cai L, Jiang G, Guo X, Yang R, Zhang Z, et al.0.25 mg Protein G crosslinked-Dynabeads were firstly incubated with 2 μg ACE2-Fc (SinoBiological, Cat#10108-H02H) or Fc (SinoBiological, Cat#10702-HNAH) protein, washed with PBST, and then incubated with 0.4 μg APOE3

(13) Human antibodies to SARS-CoV-2 with a recurring YYDRxG motif retain binding and neutralization to variants of concern including OmicronCommunications biologyJuly 29, 2022Liu H, Kaku CI, Song G, Yuan M, Andrabi R, Burton DR, Walker LM, Wilson IA.display To assess binding breadth, IgGs and recombinant human Fc-conjugated hACE2 (Sino Biological, 10108-H02H) were tested against the panel of 17 sarbecovirus RBDs expressed by yeast display as previously used17.

(14) Structural and biochemical mechanism for increased infectivity and immune evasion of Omicron BA.2 variant compared to BA.1 and their possible mouse originsCell researchJuly 1, 2022Xu Y, Wu C, Cao X, Gu C, Liu H, Jiang M, Wang X, Yuan Q, Wu K, Liu J, et al.SARS-CoV-2 spike ECD were evaluated using Octet Red96e (Sartorius). The dimeric human ACE2 (Sino Biological, 10108-H02H) was immobilized on Protein A biosensor (Sartorius, 18-5012) followed by measuring the association and

(15) Peptide-Based Vaccine against SARS-CoV-2: Peptide Antigen Discovery and Screening of Adjuvant SystemsPharmaceuticsApril 13, 2022Ahmed O. Shalash et al.2019-nCoV spike RBD-His recombinant protein) were obtained from Sino Biologicals (Beijing, China, Cat. No. 10108-H02H). Synthesis and Purification of Peptide Antigens Antigen and conjugate synthesis were conducted using

(16) Rapid and Quantitative In Vitro Evaluation of SARS-CoV-2 Neutralizing Antibodies and NanobodiesAnalytical chemistryMarch 15, 2022Wu W, Tan X, Zupancic J, Schardt JS, Desai AA, Smith MD, Zhang J, Xie L, Oo MK, Tessier PM, et al.SuperBlock™ (PBS) buffer (37515) were purchased from Thermo Fisher. Human recombinant ACE2 protein (hFc Tag, 10108-H02H), SARS-CoV-2 (2019-nCoV) Spike RBD-His recombinant protein (40592-V08H), and SARS-CoV spike/S1 protein (S1 subunit, His tag

(17) Egg yolk immunoglobulin (IgY) targeting SARS-CoV-2 S1 as potential virus entry blockerJournal of applied microbiologyMarch 1, 2022Bao L, Zhang C, Lyu J, Yi P, Shen X, Tang B, Zhao H, Ren B, Kuang Y, Zhou L, et al.(BLI) to study the binding kinetics and affinity of S1 to human angiotensin‐converting enzyme 2 (hACE2; 10108‐H02H, Sino Biological) on an Octet K2 instrument (FortéBio; Wang et al., 2020; Yang et al., 2020). First,

(18) Omicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2bioRxiv : the preprint server for biologyFebruary 28, 2022Fang Z, Peng L, Filler R, Suzuki K, McNamara A, Lin Q, Renauer PA, Yang L, Menasche B, Sanchez A, et al.

(19) Improvement of native structure-based peptides as efficient inhibitors of protein-protein interactions of SARS-CoV-2 spike protein and human ACE2Frontiers in molecular biosciencesJanuary 1, 2022Odolczyk N, Klim J, Podsiadła-Białoskórska M, Winiewska-Szajewska M, Szolajska E, Zielenkiewicz U, Poznański J, Zielenkiewicz P.HEK293 cells with an hFc tag attached to the C-terminus was provided by SinoBiological. cat. no. SIN-10108-H02H (purity ≥95%). The peptides: DKGNHE (pep1d) and DYGNHE (J3), DYGNYE (J3.1), MYGNHE (J3.2), LYGNHE (J3.3),

(20) Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA AssayVaccinesDecember 16, 2021Shalash AO, Azuar A, Madge HYR, Modhiran N, Amarilla AA, Liang B, Khromykh AA, Watterson D, Young PR, Toth I, et al.P1379);Recombinant human ACE2 with human IgG-Fc (hFc-ACE2, Sino Biological, Beijing, China, Cat. No. 10108-H02H);Sodium hydrogen carbonate (Chem Supply, South Australia, Australia, Cat. No. SA001-500G);Sodium carbonate

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Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag)

Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) from Sino Biological, Inc.

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