Human CD32b / FCGR2B Protein (His Tag) 10259-H08H from Sino Biological, Inc.

Product Specs
ItemHuman CD32b / FCGR2B Protein (His Tag)
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10259-H08H
Activity1.Loaded Recombinant Human CD32B/Fcgr2b Protein, His Tag (Cat. No. 10259-H08H) on His1K Biosensor, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 1.13 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Anti-CD20 (Research Grade Rituximab Biosimilar) captured on Protein A chip can bind Recombinant Human CD32B/Fcgr2b Protein, His Tag (Cat. No. 10259-H08H) with an affinity constant of 0.7827 µM as determined in an SPR assay (Biacore T200) (Routinely tested). 3.Recombinant Human CD32b / FCGR2B Protein (His Tag) (Cat: 10259-H08H) captured on CM5 chip via Anti-His antibody, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 12.130 µM as determined in an SPR assay (Biacore T200) (QC tested)
FormatLyophilized from sterile PBS, pH 7.4
Size100 µg
NCBI Gene AliasesCD32, CD32B, FCG2, FCGR2, FCGR2C, FcGRIIB, FcRII-c, FcgammaRIIb, IGFR2
NCBI Full Gene NameFc gamma receptor IIb
Source/Expression SystemHEK293 Cells
Molecule NameFCGR2B
SpeciesHuman
Molecular WeightThe recombinant mature human CD32b consists of 183 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 20.8 kDa. By SDS-PAGE under reducing conditions, it migrates as an approximately 25-30 kDa protein due to glycosylation
TypeRecombinant protein
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
Conjugate/TagC-His

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Human Fcγ-receptors selectively respond to C-reactive protein isoformsFrontiers in immunologyJanuary 1, 2025Henning A, Seer J, Zeller J, Peter K, Chen H, Thomé J, Kolb P, Eisenhardt SU, Hoffmann K, Hengel H.Bejing, China, recombinant, HEK293/ECD, C-terminal polyhistidine tag: 1038-H08H1/10374-H08H1/10374-H08H/10259-H08H/10256-H08H/10389-H08C) to immobilized pCRP (US Biological Life Sciences) or IgG (human IgG1 kappa, #

(2) Systematic analysis of Fc mutations designed to reduce binding to Fc-gamma receptorsmAbsDecember 31, 2024Geoff Hale, Jelle De Vos, Alastair Douglas Davy, Koen Sandra, Ian Wilkinson,Sino Biological,10374-H08H FcγRIIa (CD32a) 131 H allele,human,Sino Biological,10374-H08H1 FcγRIIb (CD32b),human,Sino Biological,10259-H08H FcγRIIIa (CD16a) 158F allele,human,Sino Biological,10389-H08H FcγRIIIa (CD16a) 158 V allele,human,Sino Biological

(3) FcγRIIB Is an Immune Checkpoint Limiting the Activity of Treg-Targeting Antibodies in the Tumor MicroenvironmentCancer immunology researchMarch 4, 2024Knorr DA, Blanchard L, Leidner RS, Jensen SM, Meng R, Jones A, Ballesteros-Merino C, Bell RB, Baez M, Marino A, et al.(#11159-HNAH, Sino Biological), and recombinant human FcγRs (human FCGR2A #10374-H08H, human FCGR2B #10259-H08H, human FCGR3A #10389-H08H, Sino Biological). Ninety-six–well ELISA Half Area High Binding plates (#675061,

(4) PvDBPII elicits multiple antibody-mediated mechanisms that reduce growth in a Plasmodium vivax challenge trialNPJ vaccinesJanuary 6, 2024Martinez FJ, White M, Guillotte-Blisnick M, Huon C, Boucharlat A, Agou F, England P, Popovici J, Hou MM, Silk SE, et al.(FcγR) Ia (FcγRIa), FcγRIIa, FcγRIIb, and FcγRIIIa (all from Sino Biological: 10256-H08H, 10374-H08H1, 10259-H08H and 10389-H08H1, respectively) at dilutions 19 nM, 1.7 μM, 3.7 μM and 2.3 μM, respectively. The method

(5) FcyRIIB is a novel immune checkpoint in the tumor microenvironment limiting activity of Treg-targeting antibodiesbioRxivJuly 4, 2023David Knorr, Rom Leidner, Shawn Jensen, Ryan Meng, Andrew Jones, Carmen Ballesteros-Merino, R. Bryan Bell, Maria Baez, David Sprott, Carlo Bifulco, et al.human CTLA-4 (Sino Biological, 11159-HNAH), and human FcyRs (human FCGR2A #10374-H08H, human FCGR2B #10259-H08H, human FCGR3A #10389-H08H, Sino Biological). 96 well ELISA Half Area High Binding plates (Greiner Bio-One,

(6) Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation patternCell ReportsDecember 14, 2021Inbal Farkash, Tali Feferman, Noy Cohen-Saban, Yahel Avraham, David Morgenstern, Grace Mayuni, Natasha Barth, Yaniv Lustig, Liron Miller, Dror S. Shouval, et al.FcγRIIA,Sino Biological,Cat# 10374-H08H the human recombinant receptors FcγRIIB,Sino Biological,Cat# 10259-H08H the human recombinant receptors FcγR,Sino Biological,Cat# 10389-H08H C1q,Sigma Aldrich,Cat# C1740 Nonident

(7) FcRn, but not FcγRs, drives maternal-fetal transplacental transport of human IgG antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJune 9, 2020Borghi S, Bournazos S, Thulin NK, Li C, Gajewski A, Sherwood RW, Zhang S, Harris E, Jagannathan P, Wang LX, et al.Sino Biological), FcγRIIaR131(10374-H08H1, Sino Biological), FcγRIIaH131 (10374-H08H1, Sino Biological), FcγRIIb (10259H08H, Sino Biological), FcγRIIIaV158 (10389-H08H1, Sino Biological), and FcγRIIIaF158 (10389-H08H, Sino Biological).

(8) CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive ImmunityCancer immunology researchFebruary 1, 2020de Silva S, Fromm G, Shuptrine CW, Johannes K, Patel A, Yoo KJ, Huang K, Schreiber TH.

(9) Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline StructuresFrontiers in immunologyJanuary 1, 2020Alberts A, Klingberg A, Hoffmeister L, Wessig AK, Brand K, Pich A, Neumann K.(R&D Systems, #7586-MA-050), human LDLR (Sino Biological, #10231-H08H-50), human CD32b (Sino Biological, #10259-H08H), and murine LDLR (Sino Biological, #50305-M08H). After washing with HBSS, bound proteins were detected

(10) Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodiesmAbsJuly 1, 2019Aoyama M, Hashii N, Tsukimura W, Osumi K, Harazono A, Tada M, Kiyoshi M, Matsuda A, Ishii-Watabe A.mAbs to recombinant human FcγRs with C-terminal polyhistidine tag (Cat. No. 10256-H08H, 10374-H08H1, 10259-H08H, 10389-H08H1, and 11046-H08H, Sino Biological) was assessed as described previously.47 In all experiments,

(11) Fc gamma receptor glycosylation modulates the binding of IgG glycoforms: a requirement for stable antibody interactionsJournal of proteome researchDecember 5, 2014Hayes JM, Frostell A, Cosgrave EF, Struwe WB, Potter O, Davey GP, Karlsson R, Anneren C, Rudd PM.

(12) Erwin Uehlinger on the occasion of his 80th birthday, August 8, 1979Pathology, research and practiceDecember 1, 1979Adler CP, Sandritter W.

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Human CD32b / FCGR2B Protein (His Tag)

Human CD32b / FCGR2B Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

Citations (12)

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Human CD32b / FCGR2B Protein (His Tag) from Sino Biological, Inc.

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