Human HER2 / ErbB2 Protein 10004-HCCH from Sino Biological, Inc.

Product Specs
ItemHuman HER2 / ErbB2 Protein
CompanySino Biological, Inc.
Price $358.00Supplier Page View Company Product Page
Catalog Number10004-HCCH
Size100 µg
Conjugate/TagC-cleavage
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe recombinant human ERBB2 consists 636 amino acids and predicts a molecular mass of 70 kDa. As a result of glycosylation, human ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameERBB2
Source/Expression SystemHEK293 Cells
Activity1.Immobilized Human Her2/ERBB2 (Cat:10004-HCCH) at 2 µg/ml (100 µl/well) can bind Anti-HER2(Research Grade Trastuzumab biosimilar), the EC50 is 7.0-30.0 ng/mL(QC tested). 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.5 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody(Routinely tested). 3.Loaded Anti-HER2(Research Grade Trastuzumab biosimilar) on ProA Biosensor, can bind Recombinant Human Her2 / ERBB2 Protein, no Tag (Cat. No. 10004-HCCH) with an affinity constant of 1.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Immobilized Recombinant Human HER2 / ErbB2 Protein (Cat: 10004-HCCH) at 1 µg/mL (100 µL/well) can bind Anti-HER2 (Research Grade Pertuzu*mab Biosimilar), the EC50 is 1.9 ng/mL (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4

Add to Compare List

Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Targeting HER2-Positive Solid Tumors with CAR NK Cells: CD44 Expression Is a Critical Modulator of HER2-Specific CAR NK Cell EfficacyCancers (Basel)February 21, 2025Bence Gergely, Márk A. Vereb, István Rebenku, György Vereb, Árpád Szöőrlabeled with 5 µg/mL Alexa Fluor 647-conjugated monomeric HER2 extracellular domain (HER2 ECD; Cat.#10004-HCCH, Sino Biological Europe GmbH, Eschborn, Germany) for 10 min on ice. Flow cytometric analysis was conducted

(2) Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-lifePNAS NexusFebruary 11, 2025Simone Mester, Chilam Chan, Marta Lustig, Stian Foss, J H Marco Jansen, Marie Leangen Herigstad, Mitchell Evers, Jeannette Nilsen, Karli R Reiding, J Mirjam A A. Damen, et al.number: 10544522) were coated with 1.0 μg/mL recombinant human HER2 (Sino Biological, catalog number: 10004-HCCH) diluted in PBS (Merck, catalog number: D8537), and incubated ON at 4 °C. Next, the wells were blocked

(3) Redesigning amino/carboxyl ends of DARPin G3 for high thermostability and production in tobacco transplastomic plantsPlant cell reportsAugust 10, 2024Kohnehrouz BB, Ehsasatvatan M.

(4) Exploring the Combined Action of Adding Pertuzumab to Branded Trastuzumab versus Trastuzumab Biosimilars for Treating HER2+ Breast CancerInternational Journal of Molecular SciencesApril 1, 2024Emma Franco-Mateos, Virginia Souza-Egipsy, Laura García-Estévez, José Pérez-García, María Gion, Laia Garrigós, Patricia Cortez, Cristina Saavedra, Patricia Gómez, Carolina Ortiz, et al.University Hospital Ramón y Cajal (Madrid, Spain) through one of the authors (J.C). The human HER2 (10004-HCCH) extracellular domain was purchased from Sino Biological, Inc. Buffer exchange was performed using centrifugal

(5) Modeling antibody drug conjugate potential using a granzyme B antibody fusion proteinBMC biologyMarch 14, 2024Anderson TS, McCormick AL, Smith SL, Lowe DB.

(6) CD28 and 41BB Costimulatory Domains Alone or in Combination Differentially Influence Cell Surface Dynamics and Organization of Chimeric Antigen Receptors and Early Activation of CAR T CellsCancersJune 7, 2023Mezősi-Csaplár M, Szöőr Á, Vereb G.on ice with 5 µg/mL Alexa Fluor 647-conjugated monomeric HER2 extracellular domain (HER2 ECD; Cat.#10004-HCCH, Sino Biological Europe GmbH, Eschborn, Germany) for 10 min, washed with PBS at 4 °C, and resuspended

(7) A Novel NanoMIP-SPR Sensor for the Point-of-Care Diagnosis of Breast CancerMicromachinesMay 21, 2023Erol K, Hasabnis G, Altintas Z.Methacryloxyethyl thiocarbamoyl rhodamine B was provided by Polysciences Europe (Bergstrasse, Germany). HER2-ECD (10004-HCCH) and the peptide (163–175: DTNRSRACHPCSP) chosen for imprinting were provided by Sino Biological (Eschborn,

(8) Engineering a modular double-transmembrane synthetic receptor system for customizing cellular programsCell reportsApril 25, 2023Zhou J, Ge Q, Wang D, Guo Q, Tao Y.

(9) The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncologyBiological researchOctober 23, 2022Ehsasatvatan M, Kohnehrouz BB, Gholizadeh A, Ofoghi H, Shanehbandi D.

(10) Computation-Aided Design of Albumin Affibody-Inserted Antibody Fragment for the Prolonged Serum Half-LifePharmaceuticsAugust 24, 2022Na Hyun Kwon, Jae Hun Lee, Inchan Kwon(96-well, flat, #32396) were obtained from SPL Life Sciences (Pocheon, Korea). Recombinant human HER2 (#10004-HCCH) was obtained from Sino Biological (Beijing, China) and Tween-20 (#1610781) was obtained from Bio-Rad

(11) Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteinsProceedings of the National Academy of Sciences of the United States of AmericaSeptember 28, 2021Li M, Zheng X, Shanker S, Jaroentomeechai T, Moeller TD, Hulbert SW, Koçer I, Byrne J, Cox EC, Fu Q, et al.sodium carbonate buffer (pH 9.6) for Im7 variants and 50 μL of 0.2 μg/mL HER2-ED (Sino Biological, 10004-HCCH) in PBS buffer for scFv-HER2 variants. After blocking with 5% (wt/vol) nonfat milk in PBS for 1 h at

(12) Extended plasma half-life of albumin-binding domain fused human IgA upon pH-dependent albumin engagement of human FcRn in vitro and in vivomAbsJanuary 1, 2021Mester S, Evers M, Meyer S, Nilsen J, Greiff V, Sandlie I, Leusen J, Andersen JT.anti-Her2 IgG1 variant was analyzed by capture on 1.0 μg/ml human Her2 (Sino Biological, Catalog number: 10004-HCCH), and detected by using an ALP-conjugated anti-human IgG (Fc-specific) antibody from goat (1:5000) (Sigma,

(13) A novel HER2-targeting antibody 5G9 identified by large-scale trastuzumab-based screening exhibits potent synergistic antitumor activityEBioMedicineOctober 1, 2020Ding X, Gu W, Zhong Y, Hao X, Liu J, Xia S, Luo L, Chen M, Zhang C.were immunized with BT-474 cells and recombinant human HER2-ECD protein (Sino Biological Cat# Cat: 10004-HCCH) by the standard immunization method. Mice with a high serum titer were used for hybridoma fusions.

(14) ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer patients undergoing therapy with trastuzumabScientific reportsFebruary 20, 2020Agnolon V, Contato A, Meneghello A, Tagliabue E, Toffoli G, Gion M, Polo F, Fabricio ASC.containing 150 mM sodium chloride and 0.02% sodium azide at pH 7.4. Recombinant HER2 ECD protein (code 10004-HCCH, 70 kDa), and rabbit anti-human HER2 polyclonal antibody (IgG pAb, code 10004-RP04) were obtained from

(15) Heavy chain dimers stabilized by disulfide bonds are required to promote in vitro assembly of trastuzumabBMC molecular and cell biologyJanuary 21, 2020Farràs M, Román R, Camps M, Miret J, Martínez Ó, Pujol X, Casablancas A, Cairó JJ.anti-HER2 recognition with its in vivo assembled variant. Namely, 50 μL of 50 ng/μL HER2 in PBS solution (10004-HCCH-50, SinoBiological) was placed in the wells of 96-well Maxisorp plates (ThermoFisher Scientific) for

(16) para-Sulfonatocalix[4]arene stabilized gold nanoparticles multilayers interfaced to electrodes through host-guest interaction for sensitive ErbB2 detectionBiosensors & bioelectronicsJanuary 15, 2018Wang X, Du D, Dong H, Song S, Koh K, Chen H.

(17) Molecular and hydrodynamic properties of human epidermal growth factor receptor HER2 extracellular domain and its homodimer: Experiments and multi-scale simulationsBiochimica et biophysica acta. General subjectsSeptember 1, 2017Vega JF, Ramos J, Cruz VL, Vicente-Alique E, Sánchez-Sánchez E, Sánchez-Fernández A, Wang Y, Hu P, Cortés J, Martínez-Salazar J.

(18) Novel multispecific heterodimeric antibody format allowing modular assembly of variable domain fragmentsmAbsJanuary 1, 2017Egan TJ, Diem D, Weldon R, Neumann T, Meyer S, Urech DM.(Sierra Sensors) and the following recombinant proteins (antigens): HER2 (Sino Biological, Inc., Cat. #: 10004-HCCH), CD3δε (Sino Biological, Inc., Cat. #: CT026-H0323H), IL-5R (R&D Systems, Cat. #: 253–5R), TNFα (Peprotech,

(19) Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysatesAnalytica chimica actaJanuary 28, 2016Eletxigerra U, Martinez-Perdiguero J, Barderas R, Pingarrón JM, Campuzano S, Merino S.

Be the First to Write a Review!

Human HER2 / ErbB2 Protein

Human HER2 / ErbB2 Protein from Sino Biological, Inc.

Description

Sino Biological, Inc.

Citations (19)

Reviews

Human HER2 / ErbB2 Protein from Sino Biological, Inc.

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map