Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) CT012-H08H from Sino Biological, Inc.

Description

A DNA sequence encoding the IL23A (NP_057668.1) (Met1-Pro189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified

Product Specs
ItemHuman IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag)
CompanySino Biological, Inc.
Price $58.00Supplier Page View Company Product Page
Catalog NumberCT012-H08H
Activity1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 µg/mL (100 µL/well) can bind Recombinant Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) (Cat: CT012-H08H), the EC50 is 120-400 ng/mL (QC tested). 2.Immobilized Recombinant Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) (Cat: CT012-H08H) at 2 µg/mL (100 µL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 200-600 ng/mL (Routinely tested). 3. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL. 4.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT012-H08H) with an affinity constant of 18.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5.Loaded Anti-IL-12 & IL-23 (P40)(Research Grade Ustekin*mab Biosimilar) on proA Biosensor, can bind Recombinant Human IL-23 Protein, His Tag (Cat. No. CT012-H08H) with an affinity constant of 0.532 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
Size5 µg
FormatLyophilized from sterile PBS, 10% Glycerol, pH7.4
NCBI Gene AliasesIL-23A, CLMF, IL-23, IL23P19, NKSF, NKSF2, CLMF2, P19, SGRF, IMD29, IMD28, IL-12B
NCBI Full Gene Nameinterleukin 12B, interleukin 23 subunit alpha
Source/Expression SystemHEK293 Cells
Molecule NameIL12B & IL23A
SpeciesHuman
Molecular WeightThe recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181+317) amino acids and has a calculated molecular mass of 56.3 (20.1+36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22.6 & 42.8 kDa respectively in SDS-PAGE under reducing conditions
TypeRecombinant protein
Purity> 90 % as determined by SDS-PAGE
Conjugate/TagC-His

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Preclinical development of a bispecific TNFα/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel diseaseScientific reportsSeptember 30, 2021Roberts KJ, Cubitt MF, Carlton TM, Rodrigues-Duarte L, Maggiore L, Chai R, Clare S, Harcourt K, MacDonald TT, Ray KP, et al.Recombinant human MMP3 (513-MP-010) and MMP12 (917-MP-010), from Sino Biologicals; Recombinant Human IL-23 (CT012-H08H), Recombinant cynomolgus monkey IL-23R-Fc (90123-C02H), Murine IL-23 (CT028-M08H), Recombinant marmoset

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Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag)

Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) from Sino Biological, Inc.

Description

Sino Biological, Inc.

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Human IL-23 (IL23A & IL12B Heterodimer) Protein (His Tag) from Sino Biological, Inc.

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