Human PD1 / PDCD1 Protein (His Tag) 10377-H08H from Sino Biological, Inc.

Product Specs
ItemHuman PD1 / PDCD1 Protein (His Tag)
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10377-H08H
Activity1.Immobilized human PD-1 (Cat:10377-H08H) at 2 µg/ml (100 µl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10084-H02H), the EC50 of human B7-H1 is 150-600 ng/mL(QC tested). 2.Immobilized human PD-1 (Cat:10377-H08H) at 2 µg/mL (100 µL/well) can bind Anti-PD1(Research grade Nivolumab biosimilar),the EC50 is 8-60 ng/mL(Routinely tested). 3.Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Recombinant Human PD1 Protein, His Tag (Cat. NO. 10377-H08H) with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind PD1 (Cat.No.10377-H08H) with an affinity constant of 10.03nM as determined in a SPR assay (Biacore T200)(Routinely tested). 5.Loaded Recombinant Human PD-L2/B7-DC Protein, hFc Tag (Cat. No. 10292-H02H) on proA Biosensor, can bind Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) with an affinity constant of 0.435 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 6.Loaded Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) on HIS1K Biosensor, can bind Recombinant Human PD-L2/B7-DC Protein, hFc Tag (Cat. No. 10292-H02H) with an affinity constant of 19.400 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 7.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat: 10377-H08H) at 2 µg/mL (100 µL/well) can bind Recombinant Human PD-L2 / PDCD1LG2 Protein (ECD, mFc Tag) (Cat: 10292-H38H), the EC50 is 87.7 ng/mL (Routinely tested). 8.Loaded Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H) on His1K Biosensor, can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat. No. 10084-H02H) with an affinity constant of 69.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 9.Loaded Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag) (Cat. No. 10084-H05H) on AMC Biosensor, can bind Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H) with an affinity constant of 1.57 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
Size100 µg
FormatLyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0
NCBI Gene AliasesCD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
NCBI Full Gene Nameprogrammed cell death 1
Source/Expression SystemHEK293 Cells
Molecule NamePDCD1
SpeciesHuman
Molecular WeightThe recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions
TypeRecombinant protein
Purity> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Conjugate/TagC-His

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Teriflunomide modulates the PD-1/PD-L1 axis and enhances antitumor immunity in colorectal cancerOncogenesisMarch 18, 2026Jung Ho Han, Eun-Ji Lee, Young-Hoon Park, Jung-Hye Ha, Kazi Rejvee Ahmed, Jang-Gi Choi, Hwan-Suck ChungHealthCare). The NTA surfaces were washed with 350 mM EDTA and loaded with 0.5 mM NiCl2. The hPD-1 protein (#10377-H08H, Sino Biologicals) was bound to the NTA sensor chip using an affinity capture method. Various concentrations

(2) De novo design of epitope-specific antibodies via a structure-driven computational workflowNature CommunicationsDecember 10, 2025Fandi Wu, Yu Zhao, JiaXiang Wu, Biaobin Jiang, Bing He, Longkai Huang, Chenchen Qin, Yang Xiao, Fan Yang, Rubo Wang, et al.interaction interface to block their binding and prevent immune evasion by tumors. (Sino Biological, REF:10377-H08H). Programmed death-ligand 1 (PD-L1), a cell-surface receptor that interacts with PD-1, plays a key role

(3) Self-assembly of hybrid 3D cultures by integrating living and synthetic cellsNat CommunDecember 10, 2025Nils Piernitzki, Ning Gao, Gilles Gasparoni, Louisa M. Krauß, Julia Schulze-Hentrich, Michael Dustin, Bianca Schrul, Balázs Győrffy, Stephen Mann, Oskar Stauferrec. proteins,source,catalog nr.,NCBI ref. sequence "rec. hu. PD-1 (ECD, His Tag)",Sino Biological,10377-H08H,NP_005009.2 "rec. hu. CD2 (ECD, His Tag)",Sino Biological,10982-H08H,NP_001758.2 rec. hu. CD27 (His

(4) Mass Spectrometry-Driven Epitope Mapping: Application of Diethylpyrocarbonate Covalent Labeling for the Immunotherapeutic Target Programmed Cell Death Protein 1Journal of the American Society for Mass SpectrometryJuly 2, 2025Parawan Ramanandana, Phumrapee Pianpaktr, Jiradej Makjaroen, Tossapon Wongtangprasert, Zachary J. Kirsch, Eric M. Graban, Richard W. Vachet, Poorichaya Somparn, Trairak Pisitkun, Patanachai K. Limpikirati3-(N-morpholino)propanesulfonic acid (MOPS), 500 mM NaCl, and 7.5% trehalose at pH 7.0 (Cat. No. 10377-H08H) was ordered from Sino Biological (Beijing, China). The protein was expressed in HEK293 cells. Nivolumab

(5) A Bifunctional Antibody Targeting PD-1 and TGF-β Signaling Has Antitumor Activity in Combination with Radiotherapy and Attenuates Radiation-Induced Lung InjuryCancer immunology researchMay 2, 2025Wang S, Xu D, Wang Y, Zhou Y, Xiao L, Li F, Tu J, Qin W, Tian S, Zheng B, et al.from 10−1 to 106 ng/mL, and the plates were incubated. The plates were then washed, biotin-labeled (10377-H08H, Sino Biological) PD-1 protein was added, and the plates were incubated. The plates were washed and

(6) Functional differences between rodent and human PD-1 linked to evolutionary divergence*Science ImmunologyJanuary 3, 2025Takeya Masubuchi, Lin Chen, Nimi Marcel, George A. Wen, Christine Caron, Jibin Zhang, Yunlong Zhao, Gerald P. Morris, Xu Chen, Stephen M. Hedrick, et al.purchased from Invitrogen. BSA (#A-420-1) was purchased from Goldbio. Recombinant protein huPD-1-His (#10377-H08H), huPD-L1-His (#10084-H08H), moPD-1-His (#50124-M08H), moPDL1-His (#50010-M08H), huICAM-1 (#10346-H08H),

(7) PD-1 antibody interactions with Fc gamma receptors enable PD-1 agonism to inhibit T cell activation - therapeutic implications for autoimmunityJournal of autoimmunityDecember 1, 2024Feng Y, Deyanat-Yazdi G, Newburn K, Potter S, Wortinger M, Ramirez M, Truhlar SME, Yachi PP.

(8) PD-L1 targeted peptide demonstrates potent antitumor and immunomodulatory activity in cancer immunotherapyFrontiers in immunologyJanuary 1, 2024Liang Y, Luo H, Li X, Liu S, Habib A, Liu B, Huang J, Wang J, Yi H, Hu B, et al.10084-HNAH), biotinylated mouse PD-1/PDCD1 protein (His tag) and recombinant human PD-1 protein (Cat: 10377-H08H) were purchased from Sino Biological Inc (Beijing). GM-CSF (Cat: 315-03) and Murine TNF-α (Cat: 315-01A)

(9) Resistance to anti-PD-1/anti-PD-L1: galectin-3 inhibition with GB1211 reverses galectin-3-induced blockade of pembrolizumab and atezolizumab binding to PD-1/PD-L1Frontiers in ImmunologyAugust 28, 2023Joseph Mabbitt, Ian D. Holyer, James A. Roper, Ulf J. Nilsson, Fredrik R. Zetterberg, Lynda Vuong, Alison C. Mackinnon, Anders Pedersen, Robert J. Slacktreatment with an anti-PD-L1 blocking antibody. Methods Materials PD-1 (10084-H08H) and PD-L1 (10377-H08H) human recombinant proteins were purchased from Sino Biological (China). Jurkat-Lucia™ TCR-hPD-1 and

(10) A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cellsFrontiers in Bioengineering and BiotechnologyJune 22, 2023Asim Azhar Siddiqui, Sabrina Peter, Eve Zi Xian Ngoh, Cheng-I. Wang, Shirelle Ng, John A. Dangerfield, Walter H. Gunzburg, Peter Dröge, Harshyaa Makhijaperformed at room temperature with constant shaking. Biotinylated recombinant human PD-1 (Sino Biological# 10377-H08H) was immobilized on the neutravidin (ThermoFisher scientific, #31000)-coated ELISA plate, to which the

(11) Thorectidiol A Isolated from the Marine Sponge Dactylospongia elegans Disrupts Interactions of the SARS-CoV-2 Spike Receptor Binding Domain with the Host ACE2 ReceptorJournal of natural productsMarch 24, 2023Williams DE, Cassel J, Zhu JL, Yang JX, de Voogd NJ, Matainaho T, Salvino JM, Wang YA, Montaner LJ, Tietjen I, et al.

(12) Repurposing pentamidine for cancer immunotherapy by targeting the PD1/PD-L1 immune checkpointFrontiers in immunologyJanuary 1, 2023Gu T, Tian X, Wang Y, Yang W, Li W, Song M, Zhao R, Wang M, Gao Q, Li T, et al.T-200 (GE Healthcare, UK) equipped with a research-grade CM5 sensor chip (GE Healthcare, UK). PD-1 (Cat#10377-H08H, Sino Biological, Beijing, China) or PD-L1 (Cat#10084-H08H, Sino Biological, Beijing, China) proteins

(13) Oncolytic herpes simplex virus delivery of dual CAR targets of CD19 and BCMA as well as immunomodulators to enhance therapeutic efficacy in solid tumors combined with CAR T cell therapyFrontiers in oncologyJanuary 1, 2022Liu Y, Zheng Y, Deng T, Huang Y, Liu Z, Zhan B, Zhou X, Yan R, Ren J, Xing Y, et al.concentration of anti-human PD-1 antibody was measured as follows: Recombinant his-tagged PD-1 protein (Cat. 10377-H08H, Sino Biological, China) was coated onto the 96-well ELISA plates at 100 ng/well overnight at 4°C. The

(14) A Small Molecule Antagonist of PD-1/PD-L1 Interactions Acts as an Immune Checkpoint Inhibitor for NSCLC and Melanoma ImmunotherapyFrontiers in immunologyJanuary 1, 2021Wang Y, Gu T, Tian X, Li W, Zhao R, Yang W, Gao Q, Li T, Shim JH, Zhang C, et al.device and continued to prime twice with filtered phosphate-buffered saline (PBS). The PD-1 protein (Cat#10377-H08H, Sino Biological, Beijing, China) was immobilized using amine-coupling chemistry according to the wizard

(15) Development and Evaluation of Novel Aptamers Specific for Human PD1 Using Hybrid Systematic Evolution of Ligands by Exponential Enrichment ApproachImmunological investigationsJuly 1, 2020Khedri M, Abnous K, Rafatpanah H, Nabavinia MS, Taghdisi SM, Ramezani M.

(16) Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitorsEMBO molecular medicineJune 8, 2020Fan Z, Tian Y, Chen Z, Liu L, Zhou Q, He J, Coleman J, Dong C, Li N, Huang J, et al.μg/ml) overnight at 4°C. After blocking, recombinant 6×His‐tagged hPD‐1 (500 ng/ml, Sino Biological, 10377‐H08H‐50) or 6×His‐tagged mPD‐1 protein (500 ng/ml, Sino Biological, 50124‐M08H‐100) was added and plates

(17) Enhanced Therapeutic Efficacy of a Novel Oncolytic Herpes Simplex Virus Type 2 Encoding an Antibody Against Programmed Cell Death 1Molecular therapy oncolyticsDecember 20, 2019Zhu Y, Hu X, Feng L, Yang Z, Zhou L, Duan X, Cheng S, Zhang W, Liu B, Zhang K.China). For the analysis of binding ability of the expressed anti-hPD1mAb, human PD-1 protein (His-tag) (10377-H08H; Sino Biological Incorporation, Beijing, China) and a His-tagged protein (a gift from Prof. Qun Wei,

(18) PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysImmunityDecember 17, 2019Zhao Y, Lee CK, Lin CH, Gassen RB, Xu X, Huang Z, Xiao C, Bonorino C, Lu LF, Bui JD, et al.study,N/A Strep-SNAP-CTLA-4-GCN4-His6,This study,N/A SNAP-CTLA-4-His6,This study,N/A Human PD-1-His,Sino Biological,Cat# 10377-H08H Human PD-L1-His,Sino Biological,Cat# 10084-H08H Human PD-L1,R&D systems,Cat # 9049-B7 EZ-Link NHS-LC-Biotin,Thermo Fisher Scientific

(19) PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor modelsOncotargetMarch 19, 2019Mitchell LA, Yagiz K, Hofacre A, Viaud S, Munday AW, Espinoza FL, Mendoza D, Rodriguez-Aguirre ME, Bergqvist S, Haghighi A, et al.PD-L1-Fc coated wells were co-incubated with 1 µg/mL His-tagged recombinant human PD-1 (SinoBiological, # 10377-H08H) or His-tagged recombinant mouse PD-1 (abcam, # ab180051) in competition with supernatant of undefined

(20) Enzyme linked immunosorbent assay for the quantification of nivolumab and pembrolizumab in human serum and cerebrospinal fluidJournal of pharmaceutical and biomedical analysisFebruary 5, 2019Pluim D, Ros W, van Bussel MTJ, Brandsma D, Beijnen JH, Schellens JHM.

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Human PD1 / PDCD1 Protein (His Tag)

Human PD1 / PDCD1 Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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Human PD1 / PDCD1 Protein (His Tag) from Sino Biological, Inc.

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