Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) 10084-H02H from Sino Biological, Inc.

Product Specs
ItemHuman PD-L1 / B7-H1 / CD274 Protein (Fc Tag)
CompanySino Biological, Inc.
Price $2,188.00Supplier Page View Company Product Page
Catalog Number10084-H02H
Size1 mg
Conjugate/TagC-human IgG1-Fc
Purity≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested)
TypeRecombinant protein
Molecular WeightThe recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 121.9 kDa verified by SEC-MALS(Routinely tested)
SpeciesHuman
Molecule NameCD274
Source/Expression SystemHEK293 Cells
Activity1.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat:10377-H08H) at 2 µg/mL (100 µL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat:10084-H02H), the EC50 is 220-660 ng/mL(Routinely tested). 2.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10084-H02H) at 5 µg/mL (100 µL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 100-350 ng/mL (QC tested). 3.Loaded Recombinant Human PD-1 Protein, His tag (Cat. No. 10377-H08H) on His1K Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) with an affinity constant of 0.11 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.CHO cells were transduced with human PD1 and subjected to flow cytometric analysis using recombinant human PDL1 protein (Fc tag) (left, Cat. No. 10084-H02H, red) and a negative control protein (blue). The cells were then stained with an FITC-conjugated anti-human IgG Fc antibody. Non-transduced CHO cells were used as a control (right)(Routinely tested). 5.Loaded Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) on proA Biosensor, can bind Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) with an affinity constant of 8.110 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 6.Loaded Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein, His Tag (Cat. No. 10698-H08H) with an affinity constant of 24.600 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 7. Immobilized Recombinant Human PD-L1/B7-H1 Protein (ECD, hFc Tag), HPLC-verified (Cat: 10084-H02H) at 5 µg/mL (100 µL/well) can bind Recombinant Human B7-1/CD80 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 10698-H41H-B) the EC50 is 571 ng/mL (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Design of a bispecific peptide-nanozyme conjugate for cancer immunotherapyCell Reports MedicineFebruary 17, 2026Danhong Chen, Ran Xu, Xiaoyun Ye, Youmei Xiao, Mengfan Wang, Wanqiong Li, Feiyu Luo, Xiaoshuang Niu, Xin Yang, Ye Su, et al.(EDC),Aladdin,E106172 OVA antigen peptide (SIINFEKL),Laboratory synthesis,N/A Human PD-L1-Fc fusion protein,SinoBiological,10084-H02H Murine PD-L1-Fc fusion protein,SinoBiological,50010-M02H Lipopolysaccharide (LPS),Sigma-Aldrich,L2880 murine

(2) Design, synthesis, anti-tumor activity and mechanism of novel PROTACs as degraders of PD-L1 and inhibitors of PD-1/PD-L1 interactionBioorganic & medicinal chemistrySeptember 1, 2024Zhang F, Yu Q, Wu C, Sun S, Wang Y, Wang R, Chen Z, Zhang H, Xiong X, Awadasseid A, et al.

(3) Human cytomegalovirus UL23 exploits PD-L1 inhibitory signaling pathway to evade T cell-mediated cytotoxicitymBioJuly 17, 2024Yuan Q, Fan Z, Huang W, Huo X, Yang X, Ran Y, Chen J, Li H.clone P3.6.2.8.1). Recombinant PD-L1 protein (PD-L1 Fc; Sino Biological, Beijing, China; catalog number 10084-H02H). RNA sequencing Total RNA from mock and HCMV (Towne, Towne-ΔUL23)-infected HFFs was extracted using

(4) Dual targeting of TIGIT and PD-1 with a novel small molecule for cancer immunotherapyBiochemical pharmacologyMay 1, 2024Li Y, Li B, Wang Q, Zhang X, Zhang Q, Zhou X, Shi R, Wu Y, Zhai W, Chen Z, et al.

(5) The Nucleocapsid Protein of SARS-CoV-2, Combined with ODN-39M, Is a Potential Component for an Intranasal Bivalent Vaccine with Broader FunctionalityVirusesMarch 8, 2024Lobaina Y, Chen R, Suzarte E, Ai P, Huerta V, Musacchio A, Silva R, Tan C, Martín A, Lazo L, et al.

(6) ICAM-1 mediated cell-cell adhesion exerts dual roles on human B cell differentiation and IgG productioniScienceNovember 22, 2023Shuai Liu, Zhi-cui Liu, Mei-yu Zhang, Shu-jun Wang, Meng Pan, Ping Ji, Cheng Zhu, Ping Lin, Ying WangTRIzol Reagent,Invitrogen,Cat# 15596018 Trypan Blue,Gibco,Cat# 15250061 Recombinant Human PD-L1-Fc,SinoBiological,Cat# 10084-H02H Fixation/Perm Diluent,eBioscience,Cat# 00-5223-56 Fixation/Permeabilization Concentrate,eBioscience,Cat#

(7) Thorectidiol A Isolated from the Marine Sponge Dactylospongia elegans Disrupts Interactions of the SARS-CoV-2 Spike Receptor Binding Domain with the Host ACE2 ReceptorJournal of natural productsMarch 24, 2023Williams DE, Cassel J, Zhu JL, Yang JX, de Voogd NJ, Matainaho T, Salvino JM, Wang YA, Montaner LJ, Tietjen I, et al.

(8) A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cellsTranslational oncologyJuly 1, 2022Li Y, Wu L, Liu Y, Ma S, Huang B, Feng X, Wang H.repeated at least three times. For PD-L1 ELISA analysis, recombinant human PD-L1 (Sino Biological, cat# 10084-H02H) or recombinant mouse PD-L1 (Sino Biological, cat# 50010-M02H) (0.1 µg/well) was coated at 4 °C overnight,

(9) Conditional PD-1/PD-L1 Probody Therapeutics Induce Comparable Antitumor Immunity but Reduced Systemic Toxicity Compared with Traditional Anti-PD-1/PD-L1 AgentsCancer immunology researchDecember 1, 2021Assi HH, Wong C, Tipton KA, Mei L, Wong K, Razo J, Chan C, Howng B, Sagert J, Krimm M, et al.using recombinant His-tagged human or mouse PD-L1 extracellular domain (Sino Biological, catalogs #10084-H02H and #50010-M08H, respectively). Five unique scFv clones that bound human PD-L1 (hPD-L1) extracellular

(10) Novel anti-4-1BB×PD-L1 bispecific antibody augments anti-tumor immunity through tumor-directed T-cell activation and checkpoint blockadeJournal for immunotherapy of cancerJuly 1, 2021Jeong S, Park E, Kim HD, Sung E, Kim H, Jeon J, Kim Y, Jung UJ, Son YG, Hong Y, et al.measured using a microplate reader (Molecular Devices). For the dual-antigen capture ELISA, human PD-L1 (10084-H02H; Sino Biological) was coated and ABL503 binding detected using human 4-1BB-His (10041-H08H; Sino Biological)

(11) Soluble PD-L1 is associated with local and systemic inflammation markers in primary and secondary brain tumoursESMO openNovember 1, 2020Mair MJ, Pajenda S, Ilhan-Mutlu A, Steindl A, Kiesel B, Widhalm G, Dieckmann K, Feldmann K, Hainfellner J, Marosi C, et al.subsequent washes with PBS, samples and standard PD-L1 human recombinant protein (Sino Biologicals 10084-H02H, Wayne, Pennsylvania, USA) were incubated for 12 hours at 4°C. The plate was rinsed with wash buffer

(12) Repositioning liothyronine for cancer immunotherapy by blocking the interaction of immune checkpoint TIGIT/PVRCell communication and signaling : CCSSeptember 7, 2020Zhou X, Du J, Wang H, Chen C, Jiao L, Cheng X, Zhou X, Chen S, Gou S, Zhao W, et al.

(13) Gated Resonance Energy Transfer (gRET) Controlled by Programmed Death Protein Ligand 1Nanomaterials (Basel, Switzerland)August 13, 2020Grel H, Ratajczak K, Jakiela S, Stobiecka M.

(14) Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitorsEMBO molecular medicineJune 8, 2020Fan Z, Tian Y, Chen Z, Liu L, Zhou Q, He J, Coleman J, Dong C, Li N, Huang J, et al.aCD3/aCD28 and with culture media supplemented with 10 μg/ml hPD‐L1 protein (Fc tag, Sino Biological, 10084‐H02H‐100) in the presence of 100 nM of MB or 25 μg/ml of pembrolizumab or 20 μg/ml nivolumab (kindly gifted

(15) Engineering of a novel subnanomolar affinity fibronectin III domain binder targeting human programmed death-ligand 1Protein engineering, design & selection : PEDSDecember 31, 2019Ramakrishnan S, Natarajan A, Chan CT, Panesar PS, Gambhir SS.biotinylation Recombinant human (rh) B7-H1/Fc chimera was purchased from Sino Biologicals (Cat. No 10084-H02H, Beijing, China) and biotinylated using EZ-LinkTM NHS-PEG4 -Biotinylation Kit (Thermo Fisher Scientific,

(16) Induction of Follicular-Like CXCR5⁺ CD8⁺ T Cells by TGF-β1/IL-23 Is Limited During HIV InfectionViral immunologySeptember 1, 2019Perdomo-Celis F, Feria MG, Taborda NA, Rugeles MT.

(17) Global alteration of T-lymphocyte metabolism by PD-L1 checkpoint involves a block of de novo nucleoside phosphate synthesisCell discoveryJanuary 1, 2019Palaskas NJ, Garcia JD, Shirazi R, Shin DS, Puig-Saus C, Braas D, Ribas A, Graeber TG.(Jackson Immunoresearch #009-000-008) or 1.5 µM recombinant human Fc-tagged PD-L1 (rhPD-L1.Fc) (Sino Biological #10084-H02H) in 1.2 mL PBS per well. The plate was washed twice with 2 mL PBS and blocked for 1 h at room temperature

(18) The design of high affinity human PD-1 mutants by using molecular dynamics simulations (MD)Cell communication and signaling : CCSJune 7, 2018Du J, Qin Y, Wu Y, Zhao W, Zhai W, Qi Y, Wang C, Gao Y.

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Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag)

Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

Citations (18)

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Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) from Sino Biological, Inc.

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