Human ICAM-1 / CD54 Protein (His Tag) 10346-H08H from Sino Biological, Inc.

Product Specs
ItemHuman ICAM-1 / CD54 Protein (His Tag)
CompanySino Biological, Inc.
Price $348.00Supplier Page View Company Product Page
Catalog Number10346-H08H
ActivityMeasured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 µg/ml, 100 µl/well), approximately 30%-60% will adhere specifically
Size50 µg
NCBI Gene AliasesBB2, CD54, P3.58
NCBI Full Gene Nameintercellular adhesion molecule 1
Source/Expression SystemHEK293 Cells
Molecule NameICAM1
SpeciesHuman
Molecular WeightThe recombinant human ICAM1 consists of 464 amino acids and has a predicted molecular mass of 51 kDa. As a result of glycosylation, the apparent molecular mass of rhICAM1 is approxiamtely 65-70 kDa in SDS-PAGE under reducing conditions
TypeRecombinant protein
Purity≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC
Conjugate/TagC-His
FormatLyophilized from sterile PBS, pH 7.4

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) PD1-induced Shp2 condensation organizes inhibitory signalosomes through selective substrate partitioningbioRxivMarch 14, 2026Takeya Masubuchi, George A Wen, Xiaoxian Song, Keshav Gaddam, Haian Shao, Chuan Wu, Enfu HuiBSA (Bovine Serum Albumin, #A-420-1) was purchased from Goldbio. Recombinant proteins hPDL1-His (#10084-H08H), hICAM-1 (#10346-H08H), hCD86-His (#10699-H08H), and hPVR-His (#10109-H08H) were purchased from Sino Biological. To obtain

(2) Antibody-like recognition of a γδ T cell receptor toward a foreign antigenStructure (London, England : 1993)October 2, 2025Rashleigh L, Venugopal H, Rice MT, Gunasinghe SD, Sok CL, Gherardin NA, Almeida CF, Van Rhijn I, Moody DB, Godfrey DI, et al.

(3) Functional differences between rodent and human PD-1 linked to evolutionary divergenceScience immunologyJanuary 3, 2025Masubuchi T, Chen L, Marcel N, Wen GA, Caron C, Zhang J, Zhao Y, Morris GP, Chen X, Hedrick SM, et al.(#10377-H08H), huPD-L1-His (#10084-H08H), moPD-1-His (#50124-M08H), moPDL1-His (#50010-M08H), huICAM-1 (#10346-H08H), and moICAM-1 (#50440-M08H) were purchased from SinoBiological. Recombinant protein huPD-1ECD-His (#PD1-H5221),

(4) Phase separation of chimeric antigen receptor promotes immunological synapse maturation and persistent cytotoxicityImmunityDecember 10, 2024Xu X, Chen H, Ren Z, Xu X, Wu W, Yang H, Wang J, Zhang Y, Zhou Q, Li H, et al.

(5) Structure of a fully assembled γδ T cell antigen receptorNatureOctober 1, 2024Gully BS, Ferreira Fernandes J, Gunasinghe SD, Vuong MT, Lui Y, Rice MT, Rashleigh L, Lay CS, Littler DR, Sharma S, et al.nM. NTA-functionalized lipids were coupled with 200 ng ml−1 His-tagged ICAM1 (Sino Biological, no. 10346-H08H). SLBs were repeatedly rinsed with PBS to remove excess unbound proteins. Before addition of the Jurkat

(6) Enhanced Antimalarial and Antisequestration Activity of Methoxybenzenesulfonate-Modified Biopolymers and Nanoparticles for Tackling Severe MalariaACS infectious diseasesFebruary 9, 2024Najer A, Kim J, Saunders C, Che J, Baum J, Stevens MM.μg/mL decorin (CSA-containing glycoprotein, Sigma-Aldrich, D8428) or 10 μg/mL ICAM-1 (Sino Biological, 10346-H08H) in PBS for at least overnight in a humidified chamber in the fridge.39,60 The plates were washed twice

(7) IDR-induced CAR condensation improves the cytotoxicity of CAR-Ts against low-antigen cancersbioRxivNovember 2, 2023Xinyan Zhang, Qian Xiao, Longhui Zeng, Fawzaan Hashmi, Xiaolei SuTo monitor CAR localization on the cell surface, glass was coated with 10 nM ICAM-1 (Sinobiological #10346-H08H) in PBS for 2 hrs at room temperature. CAR-T cells resuspended in imaging medium (RPMI1640 no phenol

(8) cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8(+) T cell function and anti-tumor immunityImmunityJune 13, 2023Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, Bui JD, Hui E.Lipids,Cat#: 880230C Streptavidin,ThermoFisher Scientific,Cat # S888 Human ICAM1,Sino Biological,Cat # 10346-H08H Human IL-2,PeproTech,Cat # 200-02 Human IL-15,PeproTech,Cat # 200-15 Mouse IFNγ,Biolegend,Cat # 575302 OVA257–264,Anaspec,Cat

(9) Atypical sideways recognition of CD1a by autoreactive γδ T cell receptorsNature communicationsJuly 5, 2022Wegrecki M, Ocampo TA, Gunasinghe SD, von Borstel A, Tin SY, Reijneveld JF, Cao TP, Gully BS, Le Nours J, Moody DB, et al.#13-0289-82). NTA functionalised lipids were coupled with 200 ng/ml of His-tagged ICAM-1 (Sino Biological, # 10346-H08H). SLB was repeatedly rinsed with TBS to remove excess unbound proteins. Before adding Jurkat T cells,

(10) Super-Resolution Imaging of Fas/CD95 Reorganization Induced by Membrane-Bound Fas Ligand Reveals Nanoscale Clustering Upstream of FADD RecruitmentCellsJune 12, 2022Nicholas Frazzette, Anthony C. Cruz, Xufeng Wu, John A. Hammer, Jennifer Lippincott-Schwartz, Richard M. Siegel, Prabuddha Senguptasites. Next, bilayers were incubated with monobiotinylated anti-CD3, His-tagged ICAM1 (Sino Biological 10346H08H, Beijing, China), streptavidin (Thermo Fisher Scientific SNN1001, Waltham, MA, USA) and Strep-tag Fas

(11) Mechanistic convergence of the TIGIT and PD-1 inhibitory pathways necessitates co-blockade to optimize anti-tumor CD8⁺ T cell responsesImmunityMarch 8, 2022Banta KL, Xu X, Chitre AS, Au-Yeung A, Takahashi C, O'Gorman WE, Wu TD, Mittman S, Cubas R, Comps-Agrar L, et al.50-161-4026 Human PD-L1-His,Sino Biological,Cat#: 10084-H08H Human ICAM-1-His,Sino Biological,Cat#: 10346-H08H Streptavidin,Invitrogen,Cat#: S888 SIINFEKL peptide,AnaSpec,Cat#: AS-60193-1 ,, Experimental models:

(12) Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptorseLifeNovember 4, 2021Xu X, Masubuchi T, Cai Q, Zhao Y, Hui E.PD-L1-His,Sino Biological,Cat#: 10084-H08H, Recombinant protein,Human ICAM-1-His,Sino Biological,Cat#: 10346-H08H, Recombinant protein,Streptavidin,Invitrogen,Cat#: S888, Recombinant protein,SEE super antigen,Toxin

(13) Canonical T cell receptor docking on peptide-MHC is essential for T cell signalingScience (New York, N.Y.)June 4, 2021Zareie P, Szeto C, Farenc C, Gunasinghe SD, Kolawole EM, Nguyen A, Blyth C, Sng XYX, Li J, Jones CM, et al.

(14) PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2The Journal of cell biologyJune 1, 2020Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma Y, Wang H, et al.PLBs were incubated with a mixture of 75 nM His-protein G, 3 nM human ICAM-1-His (Sino Biological; #10346-H08H), and 0.5 nM of human PD-L1–His (Sino Biological; #10084-H08H) for 1 h at 37°C. After incubation, PLBs

(15) NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancerNature communicationsApril 3, 2020Qin G, Wang X, Ye S, Li Y, Chen M, Wang S, Qin T, Zhang C, Li Y, Long Q, et al.(2.2.0.97). Transwell assay for lymphocytes Five micrometer transwell chambers were pre-coated with 20 μg mL−1 ICAM-1 (10346-H08H, SinoBiological, Wayne, PA, USA). Peripheral blood mononuclear cells (PBMCs) from health people were

(16) Imaging Chimeric Antigen Receptor (CAR) ActivationMethods in molecular biology (Clifton, N.J.)January 1, 2020Libby KA, Su X.

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Human ICAM-1 / CD54 Protein (His Tag)

Human ICAM-1 / CD54 Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

Citations (16)

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Human ICAM-1 / CD54 Protein (His Tag) from Sino Biological, Inc.

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