Mouse TNF-alpha / TNFA Protein (ECD) 50349-MNAE from Sino Biological, Inc.

Product Specs
ItemMouse TNF-alpha / TNFA Protein (ECD)
CompanySino Biological, Inc.
Price $138.00Supplier Page View Company Product Page
Catalog Number50349-MNAE
Conjugate/TagUnconjugated
Purity≥ 95 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions
SpeciesMouse
Molecule NameTNF
Source/Expression SystemE. coli
Activity1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1.5-15 pg/mL. 2.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 µg/mL (100 µL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 0.9-2.7 ng/mL (Routinely tested). 3.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 µg/mL (100 µL/well) can bind Recombinant Mouse TNFR1 / CD120a / TNFRSF1A Protein (Fc Tag) (Cat: 50496-M02H), the EC50 is 2-6 ng/mL (QC tested)
FormatLyophilized from sterile PBS, pH7.4
Size20 µg

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Sublingual Dripping Pill Formulation of Ganoderma amboinense Fruiting Body Extract Attenuates CCl4-Induced Liver Fibrosis via Multi-Pathway RegulationCurrent Issues in Molecular BiologyAugust 28, 2025Chin-Feng Liu, Chong-Ming Pan, Chun-Lin LeeMillipore, Darmstadt, Germany). Serum levels of TNF-α and IL-6 were measured with ELISA kits (Cat. Nos. 50349-MNAE and 50136-MNAE; Sino Biological Inc., North Wales, PA, USA), and CTGF concentrations with the CTGF ELISA

(2) Inflammatory macrophage-derived itaconate inhibits DNA demethylase TET2 to prevent excessive osteoclast activation in rheumatoid arthritisBone ResJune 11, 2025Kewei Rong, Dezheng Wang, Xiting Pu, Cheng Zhang, Pu Zhang, Xiankun Cao, Jinglin Zheng, Xiao Yang, Kexin Liu, Lei Shi, et al.medium supplemented with 100 ng/mL LPS (Sigma, L2880) or 100 ng/mL recombinant TNF-α (SinoBiological, 50349-MNAE). For osteoclast induction, BMMs were cultured in complete medium containing 50 ng/mL recombinant mouse

(3) Compromised endothelial Wnt/β-catenin signaling mediates the blood-brain barrier disruption and leads to neuroinflammation in endotoxemiaJournal of NeuroinflammationOctober 19, 2024Xiaowen Huang, Pengju Wei, Cheng Fang, Min Yu, Shilun Yang, Linhui Qiu, Yu Wang, Aimin Xu, Ruby Lai Chong Hoo, Junlei Changinjected intravenously with a low to high dosage of recombinant mouse TNF-α (Sinobiological, Cat. #50349-MNAE, 0.2, 0.3 or 0.4 mg/kg body weight, respectively) or vehicle control (saline) in 100 µl per mouse. All

(4) PI3Kδ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent mannerExperimental neurologyMay 1, 2024Qiu J, Wang YH, Wang XM, Chen HS.

(5) PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosisMolecular cellMarch 7, 2024Hou S, Zhang J, Jiang X, Yang Y, Shan B, Zhang M, Liu C, Yuan J, Xu D.

(6) Unveiling the potent effect of vitamin D: harnessing Nrf2/HO-1 signaling pathways as molecular targets to alleviate urban particulate matter-induced asthma inflammationBMC pulmonary medicineJanuary 25, 2024Ge D, Chen Q, Xie X, Li Q, Yang Y.

(7) CTLA-4 antibody-drug conjugate reveals autologous destruction of B-lymphocytes associated with regulatory T cell impairmenteLifeDecember 21, 2023Muthana MM, Du X, Liu M, Wang X, Wu W, Ai C, Su L, Zheng P, Liu Y.human CTLA-4 (HIS-hCTLA-4) (Sino Biological Inc, 11159-H08H). Mouse TNF-alpha (Sino Biological Inc, 50349-MNAE). IgE ELISA Kit (Thermo Fisher Scientific/Invitrogen, 88-50460-86). IgG ELISA Kit (Thermo Fisher Scientific/Invitrogen,

(8) Single-cell transcriptomic analysis reveals rich pituitary-Immune interactions under systemic inflammationPLoS biologyDecember 1, 2023Yan T, Wang R, Yao J, Luo M.or 3, 4, and 5 weeks after inflammation onset. For TNF-α treatment, mice received 0.5 mg/kg TNF-α (50349-MNAE, Sino Biological), and the pituitaries were collected at 6 h after treatment. Bulk RNA sequencing and

(9) IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγEuropean journal of pharmacologyNovember 15, 2023Lin J, Lan Y, Xiang D, Ma R, Chen Q, Ding K, Lu J.

(10) Quizartinib inhibits necroptosis by targeting receptor-interacting serine/threonine protein kinase 1FASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 1, 2023Li M, Wei J, Zhu G, Fu S, He X, Hu X, Yu Y, Mou Y, Wang J, You X, et al.

(11) Repurposing crizotinib to target RIPK1-dependent cell deathInternational immunologyMay 8, 2023Yu Y, Li M, Fu S, He X, Hu X, Zhu G, Wang J, You X, Mou Y, Ye Z, et al.

(12) Cordyceps cicadae NTTU 868 Mycelia Fermented with Deep Ocean Water Minerals Prevents D-Galactose-Induced Memory Deficits by Inhibiting Oxidative Inflammatory Factors and Aging-Related Risk FactorsNutrientsApril 19, 2023Chang CY, Yang PX, Yu TL, Lee CL.(HEA) (>95% purity) were purchased from Sigma Chemical Co. (St Louis, MO, USA). Mouse TNF-α protein (50349-MNAE), mouse IL-6 protein (50349-MNAE), mouse IL-1β protein (50349-MNAE), and rat iNOS protein (Q06518) were

(13) Repositioning linifanib as a potent anti-necroptosis agent for sepsisCell death discoveryFebruary 10, 2023Yu L, Yang K, He X, Li M, Gao L, Zha Y.Research Center on Biology and Chemistry, Shanghai); recombinant murine/human TNF-α(Sino Biological, 50349-MNAE) and (Peprotech, 300-01A-100); Linifanib (Selleck Chemicals, S1003, CAS# 796967-16-3); Pierce protease inhibitor tablets

(14) Identification of Piperlongumine as Potent Inhibitor of NecroptosisDrug design, development and therapyJanuary 1, 2023He X, Li M, Ye Z, You X, Wang J, Xiao X, Zhu G, Wei J, Zha Y.Z-VAD-fmk (Selleck Chemicals, S7023); recombinant murine/human TNF-α (Sino Biological, Beijing, China; 50349-MNAE); SMAC mimetic (SM-164, Beyotime Biotechnology, Jiangsu, China; C0114-10 mM); dimethyl sulfoxide (DMSO;

(15) Alpinia hainanensis Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and EvaluationJournal of agricultural and food chemistryApril 6, 2022Ji KL, Wu MZ, Huang CY, GongPan PC, Sun P, Sun YL, Li J, Xiao CF, Xu YK, Fan QF, et al.

(16) Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the Aβ40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer’s Disease Risk Factors and Increasing Magnesium Uptake of BrainFermentationMarch 14, 2021Yan-Zhong Wu, Chun-Lin LeeTaiwan). Aβ40 was purchased from Tocris Bioscience Co. (Ellisville, MO, USA). Mouse TNF-α protein (50349-MNAE), mouse IL-6 protein (50349-MNAE), mouse IL-1β protein (50349-MNAE), and rat iNOS protein (Q06518) were

(17) Glutaminase C Regulates Microglial Activation and Pro-inflammatory Exosome Release: Relevance to the Pathogenesis of Alzheimer's DiseaseFrontiers in cellular neuroscienceJanuary 1, 2019Gao G, Zhao S, Xia X, Li C, Li C, Ji C, Sheng S, Tang Y, Zhu J, Wang Y, et al.collected and pro-inflammatory cytokine TNF-α was measured with commercially available ELISA kits (cat# 50349-MNAE, Sino Biological) according to manufacturer’s protocols. Immunochemistry Immunochemistry was done

(18) Sharpin Controls Osteogenic Differentiation of Mesenchymal Bone Marrow CellsJournal of immunology (Baltimore, Md. : 1950)October 15, 2015Jeschke A, Catala-Lehnen P, Sieber S, Bickert T, Schweizer M, Koehne T, Wintges K, Marshall RP, Mautner A, Duchstein L, et al.serum starved overnight and then stimulated by the addition of recombinant TNF-a (Sino Biological, no. 50349-MNAE). Protein extracts were isolated by cell lysis with RIPA buffer (1% Nonidet P-40, 1% sodium deoxycholate,

(19) Sharpin contributes to TNFα dependent NFκB activation and anti-apoptotic signalling in hepatocytesPloS oneJanuary 1, 2012Sieber S, Lange N, Kollmorgen G, Erhardt A, Quaas A, Gontarewicz A, Sass G, Tiegs G, Kreienkamp HJ.

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Mouse TNF-alpha / TNFA Protein (ECD)

Mouse TNF-alpha / TNFA Protein (ECD) from Sino Biological, Inc.

Description

Sino Biological, Inc.

Citations (19)

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Mouse TNF-alpha / TNFA Protein (ECD) from Sino Biological, Inc.

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