Fig 2: Binding affinity of monomeric EphB4 to ephrin-B1 and ephrin-B2. EphB4 concentration-dependently displaced the binding of biotinylated ephrin-B1-Fc from immobilized EphB1-Fc (white circles) or the binding of biotinylated ephrin-B2-Fc from immobilized EphB4-Fc (black circles) (n = 3 independent experiments).

Fig 3: Effects of EphB4 on splenic mononuclear cells activation. TNFa production by cultured splenic mononuclear cells incubated with vehicle (Veh), cyclosporine A 1 µg/ml (CsA) or EphB4 (10–100 ng/ml) (EphB4) in the presence (black bars) or absence (white bars) of PMA (50 ng/mL) and ionomycin (500 ng/mL) (n = 4–10 independent values per group). *P < 0.05 vs. unstimulated; # P < 0.05 vs. corresponding vehicle; two-way ANOVA followed by Bonferroni’s post-test.

Fig 4: Effects of EphB/ephrinB ligands on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced inflammatory responses. Disease Activity Index (A), macroscopic score (B), colonic length (C), colonic thickness (D), colonic myeloperoxidase (MPO) (E), and lung MPO (F) activity assessed in vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg (n = 5–12 independent values per group). *P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.

Fig 5: Effects of EphB/ephrinB ligands on dextran sulphate sodium (DSS)-induced inflammatory responses. Disease Activity Index (A), colonic length (B), colonic thickness (C), colonic MPO (D) and lung MPO (E) activity assessed in vehicle-treated normal mice (N) and in DSS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg (n = 5–12 independent values per group). *P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.