Mouse M-CSF / CSF-1 Protein (ECD) 51112-MNAH from Sino Biological, Inc.

Product Specs
ItemMouse M-CSF / CSF-1 Protein (ECD)
CompanySino Biological, Inc.
Price $178.00Supplier Page View Company Product Page
Catalog Number51112-MNAH
Conjugate/TagUnconjugated
Purity≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS(Routinely tested)
TypeRecombinant protein
Molecular WeightThe recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions due to glycosylation.The molecular weight of this protein is around 71.9kDa verified by SEC-MALS (Routinely tested)
SpeciesMouse
Molecule NameCSF1
Source/Expression SystemHEK293 Cells
Activity1. Immobilized mouse CSF1 (Cat: 51112-MNAH) at 2 µg/ml (100 µl/well) can bind mouse CSF1R-Fch (Cat: 50059-M03H), The EC50 of mouse CSF1R-Fch (Cat: 50059-M03H) is 60-220 ng/mL. 2. Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 3-15 ng/mL
FormatLyophilized from sterile PBS, pH 7.4
Size20 µg

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Oxygen Permeable Dextran Nanogels as a Hemoglobin-Based Oxygen CarrierBiomater ResApril 27, 2026Jaeeun Oh, Hyemin Cho, Kidong Kim, Young-Joon Park, Yun Suk Huh, Sejin Sonbone-marrow-derived macrophages (BMDMs) by treatment with macrophage colony-stimulating factor (20 ng/ml; catalog no. 51112-MNAH, Sino Biological). On day 2 or 3 of culture, half of the medium was gently removed and added with fresh

(2) Nanoparticle-Loaded Injectable Hydrogel Alleviates Titanium Particle-Induced Osteolysis by Disrupting GATA6/DDX3X-Mediated Macrophage InflammationBiomaterials ResearchApril 16, 2026Sipeng Lin, Taihe Liu, Qi Zhu, Zhuji Ouyang, Yifan Yu, Haopeng Sun, Changchuan Li, Shixun Li, Chenhao Pan, Wing Cheuk Ko, et al.were cultivated for 5 to 7 d with the stimulation of macrophage colony-stimulating factor (M-CSF) (51112-MNAH, Sino Biological Inc.) and were cultured at 37 °C and 5% CO2. Previous studies have confirmed that sterilized

(3) CFP1-mediated H3K4me3 broad domains control early B cell lineage fate determinationNucleic Acids ResearchFebruary 23, 2026Wanyu Bai, Yalin Yang, Yanan Zhao, Shuoxu Gong, Yimiao Lu, Zhonghui Xue, Lie Wang, Fei-Long Meng, Chun Chen, Junchao Dongdensity in medium containing 25 ng ml−1 macrophage colony-stimulating factor (M-CSF) (Sino Biological, 51112-MNAH-20), 25 ng ml−1 granulocyte–macrophage colony-stimulating factor (GM-CSF) (Sino Biological, 51048-MNAH-20),

(4) Microglial HVCN1 Deficiency Improves Movement and Survival of SOD1 G93A ALS Mice by Enhancing Microglial Migration and NeuroprotectionAdvanced ScienceJanuary 4, 2026Fan Wang, Ke‐Yu Zhang, Lang‐Jian Zhu, Wei‐Jue Li, Yang Wu, Xiang Gao, Xiao‐Ru Ma, Xiu‐Hua Yin, Jian‐Bin Wu, Xiao‐Kang Ye, et al.penicillin/streptomycin (P/S, Gibco, 15140122) and 10 ng/mL macrophage colony‐stimulating factor (M‐CSF, Sino biological, 51112‐MNAH) and plated in culture dishes. Cells were maintained at 37°C under 5% CO2. Half of the medium was replaced

(5) Denosumab Regulates Titanium Particles‐Induced Macrophages Inflammation and Osteolysis by Targeting the ST18 / PARP1 InteractionOrthopaedic SurgeryDecember 29, 2025Zhipeng Chen, Fan Yang, Yifan Yu, Junxi Chen, Jichao Ye, Yue Ding10099141, Thermo Fisher Scientific, USA), 10 ng/mL recombinant macrophage colony‐stimulating factor (M‐CSF; 51112‐MNAH, Sino Biological Inc., China), and 1% penicillin–streptomycin (SV30010, Thermo Fisher Scientific, USA)

(6) From inflammation to healing: the crucial role of GPR91 activation and SDH inhibition in chronic diabetic wound recoveryStem cell research & therapyJuly 23, 2025Liu H, Zhao S, Wang H, He X, Gao S, Su M, Zhen M, Chen S, Chen L, Xie J.penicillin-streptomycin, and 20 ng/mL mouse recombinant macrophage colony-stimulating factor (MCSF, 51112-MNAH; SinoBiological). On day 3, the medium was replaced, and on day 5, the medium was replaced entirely.

(7) Inflammatory macrophage-derived itaconate inhibits DNA demethylase TET2 to prevent excessive osteoclast activation in rheumatoid arthritisBone ResJune 11, 2025Kewei Rong, Dezheng Wang, Xiting Pu, Cheng Zhang, Pu Zhang, Xiankun Cao, Jinglin Zheng, Xiao Yang, Kexin Liu, Lei Shi, et al.Biotech, C100C5). Cell viability was maintained with 50 ng/mL recombinant mouse M-CSF (SinoBiological, 51112-MNAH). For macrophage activation, BMMs were cultured in complete medium supplemented with 100 ng/mL LPS (Sigma,

(8) Siglec-15 antibody-GM-CSF chimera suppresses tumor progression via reprogramming tumor-associated macrophagesJ Immunother CancerApril 10, 2025Zemeng Ma, Xiaoyao Hao, Shuang Qu, Quanli Zhang, Jiajing Luo, Hongyan Li, Jinyu Liu, Wenwen Dai, Jun Li, Shouyong Gu, et al.Cells were cultured in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 100 ng/mL M-CSF (51112-MNAH, Sino Biological) for 5 days, with the medium changed every 2 days. To obtain TAMs, 4T-1 (for inducing

(9) Adenosine A2A Receptor Activation Alleviated Disease of Mice with Systemic Candida albicans Infection by Regulating Macrophage FunctionJ Inflamm ResMarch 6, 2025Xia-Nan Wu, Ke Dong, Yan Liu, Lan Yang, Jing Zhang, Ming Yang, Zhao-Wei GaoChina), 100 U/mL penicillin, 100 mg/mL streptomycin (Hyclone, Logan, USA) and 30 ng/mL mouse M-CSF (cat: 51112-MNAH, Sino Biological Inc., Beijing, China), and incubated for 5 days at 37°C with 5% CO2. After 5–6 days

(10) Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1AutophagyJanuary 1, 2025Huang XR, Ye L, An N, Wu CY, Wu HL, Li HY, Huang YH, Ye QR, Liu MD, Yang LW, et al.

(11) Apoptotic Breast Cancer Cells after Chemotherapy Induce Pro-Tumour Extracellular Vesicles via LAP-Competent MacrophagesRedox BiologyDecember 28, 2024Qi Zhang, Xiaodi Liu, Qiuxia Wei, Shiyu Xiong, Wanrong Luo, Yingshi zhou, Jincheng Cao, Xiaolin Xu, Rongbin Liu, Xinyu Tang, et al.anti-mouse PDL1 (Biolegend, Cat. No. 105029), Recombinant Mouse M-CSF Protein (Sino Biological, Cat. No. 51112-MNAH), Recombinant Mouse IL-4 (PeproTech, Cat. No. 214-14), GW4869 (Sigma, Cat. No. 6823-69-4), diphenyleneiodonium

(12) PKM2-mediated STAT3 phosphorylation promotes acute liver failure via regulating NLRP3-dependent pyroptosisCommunications biologyDecember 25, 2024Yu S, Pei S, Zhang M, Gao S, Chen J, Duan L, Hu E, Wang Y, Huang Y.(Shanghai, China). BMDMs were cultured in DMEM medium containing 20 ng ml−1 M-CSF (Sino Biological, 51112-MNAH), 10% fetal bovine serum (HyClone, Utah, USA), 100 units ml−1 penicillin and 100 mg ml−1 streptomycin

(13) Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppressionDevelopmental cellDecember 2, 2024Zhang C, Ma HM, Wu S, Shen JM, Zhang N, Xu YL, Li CX, He P, Ge MK, Chu XL, et al.

(14) Hexokinase 2 senses fructose in tumor-associated macrophages to promote colorectal cancer growthCell metabolismNovember 5, 2024Yan H, Wang Z, Teng D, Chen X, Zhu Z, Chen H, Wang W, Wei Z, Wu Z, Chai Q, et al.

(15) The CD163/TWEAK/Fn14 axis: A potential therapeutic target for alleviating inflammatory bone lossJournal of orthopaedic translationNovember 1, 2024Qian JK, Ma Y, Huang X, Li XR, Xu YF, Liu ZY, Gu Y, Shen K, Tian LJ, Wang YT, et al.California, USA) for 24 h. Non-adherent cells were collected and further cultured with M-CSF (30 ng/mL, 51112-MNAH, SinoBiological, Beijing, China) for 48 h to induce OCPs. In some experiments, OCPs or BMCs were stimulated

(16) CD5L up-regulates the TGF-β signaling pathway and promotes renal fibrosisLife sciencesOctober 1, 2024Chen C, Feng C, Luo Q, Zeng Y, Yuan W, Cui Y, Tang Z, Zhang H, Li T, Peng J, et al.

(17) SGLT2 inhibitor promotes ketogenesis to improve MASH by suppressing CD8(+) T cell activationCell metabolismOctober 1, 2024Liu W, You D, Lin J, Zou H, Zhang L, Luo S, Yuan Y, Wang Z, Qi J, Wang W, et al.

(18) Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasomeBone ResearchSeptember 6, 2024Yanqiu Wang, Wanqian Zhang, Yi Yang, Jinghao Qin, Ruoyu Wang, Shuai Wang, Wenjuan Fu, Qin Niu, Yanxia Wang, Changqing Li, et al.non-adherent cells were collected and cultured in complete DMEM/F12 containing 30 ng/mL M-CSF (SinoBiological, 51112-MNAH) for 7 days to obtain BMDMs. In the migration assay, 2 × 105 BMDMs in FBS-free DMEM/F12 medium were

(19) A clinical-stage Nrf2 activator suppresses osteoclast differentiation via the iron-ornithine axisCell metabolismAugust 6, 2024Dong Y, Kang H, Peng R, Liu Z, Liao F, Hu SA, Ding W, Wang P, Yang P, Zhu M, et al.

(20) Cholinergic macrophages promote the resolution of peritoneal inflammationProceedings of the National Academy of SciencesJuly 2, 2024Shufeng Luo, Huiling Lin, Chong Wu, Lan Zhu, Qiaomin Hua, Yulan Weng, Lu Wang, Xiaoli Fan, Kai-Bo Zhao, Gaoteng Liu, et al.Medium (Cat# 12200036; Thermo Fisher Scientific) supplemented with 10% FBS and 10 ng/mL M-CSF (Cat# 51112-MNAH; Sino Biological) for 7 d in order to obtain differentiated BMDMs. For stimulation, BMDMs obtained at

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Mouse M-CSF / CSF-1 Protein (ECD)

Mouse M-CSF / CSF-1 Protein (ECD) from Sino Biological, Inc.

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Sino Biological, Inc.

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Mouse M-CSF / CSF-1 Protein (ECD) from Sino Biological, Inc.

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