Fig 1: Myoferlin depletion attenuates MMP1 expression and collagen I invasion capacity of MDA-MB-231 cells.(A) Representative (n = 3) immunoblotting results of secreted MMP1 in 24 h serum starved supernatant of MDA-MB-231 lentiviral control (LTV-ctrl) and myoferlin knockdown (MYOF-KD) cells. Recombinant human matrix metalloproteinase-1 was used as a standard. Verification of myoferlin knockdown was done in the corresponding cell lysates with GAPDH as a loading control. (B) Secreted pro-MMP1 was evaluated in 231LTV-ctrl and 231MYOF-KD cells (n = 2) using ELISA (mean ± s.d., Kruskal-Wallis test/Dunn's multiple comparison analysis). (C) Results from Boyden chamber invasion assays using a coating of 3 mg/ml of rat tail collagen I to evaluate the invasive capacity of 231LTV-ctrl and 231MYOF-KD cells (n = 3, mean ± s.d., Kruskal-Wallis test/Dunn's multiple comparison analysis).