Fig 1: Depletion of Tregs Induces a Mild Systemic Inflammatory Response in the Absence of Intra-Amniotic Inflammation(A–H) Foxp3DTR dams underwent partial or total Treg depletion. Controls were injected with sterile 13 PBS. Mice were euthanized approximately 4 h after the second DT or PBS injection, and maternal plasma samples and amniotic fluid were collected. Concentrations of (A) IL-6, (B) CCL2, (C) IL-1ß, (D) TNF-a, (E) IFN?, (F) CCL7, (G) IL-22, and (H) IL-10 in the maternal plasma (n = 7 per group). Data are shown as medians with interquartile ranges and minimum/maximum ranges. C57BL/6 dams were intravenously injected with recombinant mouse IFN? (1.4 pg/100 µL), CCL7 (218 pg/100 µL), IL-22 (48 pg/100 µL), or a combination of all three. Controls were injected with 100 µL of sterile 13 PBS alone.(I) Gestational length of cytokine-injected dams. Data are shown as means with standard deviations (n = 3–6 per group).(J) Rate of early-term or full-term delivery of cytokine-injected dams (n = 3–6 per group).(K) Percentage of survival from birth until 3 weeks postpartum for neonates born to cytokine-injected dams (n = 3–6 litters per group).(L–S) Concentrations of (L) IL-6, (M) CXCL10, (N) CCL2, (O) IL-1ß, (P) TNF-a, (Q) IFN?, (R) IL-10, and (S) IL-4 in the amniotic fluid (n = 5–7 per group). Data are shown as medians with interquartile ranges and minimum/maximum ranges.Statistical analysis was performed using the Mantel-Cox test for survival curves, and Kruskal-Wallis or ANOVA tests with correction for multiple comparisons. See also Figure S5.