Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein, CF 233-FB-025/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human FGF basic/FGF2/bFGF (146 aa) Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $275.00Supplier Page View Company Product Page
Catalog Number233-FB-025/CF
SpeciesHuman
Molecule NameFGF basic/FGF2/bFGF
Source/Expression SystemE. coli-derived Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein, CF
Size25 ug (also 10 ug, 500 ug, 1 mg)
FormatLyophilized from a 0.2 µm filtered solution in Tris-HCl and NaCl
NCBI Full Gene Namefibroblast growth factor 2
NCBI Gene AliasesFGFB, HBGF-2, FGF-2, BFGF
Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Molecular WeightObserved: 17 kDa, reducing conditions
Theoretical: 16.5 kDa
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
Conjugate/TagUnconjugated
ApplicationsBioactivity

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Anti-SIA-cIgG enhances chemotherapy effectiveness through PTPN13-regulated tumor stemness in head and neck squamous cell carcinomaJournal of Translational Internal MedicineApril 28, 2026Luming Wang, Yangyang Xia, Chenshu Liu, Xiaofeng Shan, Yi Sui, Shang Xie, Zhigang Cai(#236-EG-01M, R&D Systems), and 10 ng/mL human recombinant basic fibroblast growth factor (bFGF) (#233-FB-025/CF, R&D Systems). The cells were then seeded into ultra-low attachment plates and cultured for 10 d. Spheres

(2) Patient-derived brain organoids reveal divergent neuronal activity across subpopulations of autism spectrum disorderTranslational PsychiatryFebruary 25, 2026Nisim Perets, Liya Kerem, Nir Waiskopf, Noa Horesh, Itay Goldman, Jasmine Avichzer, Doron Bril, William Tobelaim, Milcah Barashi, Liat David, et al.media (100-0276, STEMCELL Technologies) containing Y-27632 dihydrochloride (1254/10, Tocris) and FGF2 (233-FB-025/CF, R&D Systems). Cells were counted using Trypan Blue and were seeded in a non-treated U-shape 96-well

(3) Pathophysiology of androgen-associated endothelial cell dysfunction in phenotype A polycystic ovarian syndrome revealed by iPSCs modelingFront Endocrinol (Lausanne)February 12, 2026Chia-Eng Wu, Chu-Chun Huang, Yung-Jen Hsiao, Chia-Lang Hsu, Tzu-Hsin Chen, Mei-Jou Chen, Hong-Nerng Howith 10 ng/mL basic fibroblast growth factor (bFGF) (R&D systems, Minneapolis, MN, USA; catalog number 233-FB-025/CF). The medium was changed every day. The cells were split weekly using 30-gauge insulin needles (Terumo

(4) Impaired Complex I dysregulates neural/glial precursors and corpus callosum development revealing postnatal defects in Leigh syndrome miceEMBO Molecular MedicineDecember 22, 2025Sahitya Ranjan Biswas, Porter L Tomsick, Colin Kelly, Brooke A Lester, Julia P Milner, Sara N Henry, Yairis Soto, Samantha Brindley, Nicole DeFoor, Paul D Morton, et al.(Fisher Scientific #17502048), EGF (20 ng/μL, R&D Systems # 236-EG-200), and FGF (20 ng/μL, R&D Systems # 233-FB-025/CF) from P14 animals. Cells were plated in ultra-low adherent 24-well culture plates (Fisher Scientific

(5) A tissue-engineered endothelial cell – smooth muscle cell arteriole-like modelBiomaterials ScienceNovember 4, 2025Ninghao Zhu, Lily Liang, Nan Zhao, Haosong Chen, Peter C. Searsonmedium (VMM): SMCM supplemented with 2 ng mL−1 human basic fibroblast growth factor (bFGF, R&D Systems, 233FB025CF, Minneapolis, MN), 0.5 ng mL−1 human epidermal growth factor (EGF, AF10015100), 5 ng mL−1 heparin, 0.01

(6) Chromatin activity of IκBα mediates the exit from naïve pluripotencyeLifeOctober 22, 2025Palma LG, Alvarez-Villanueva D, Maqueda M, Barrero M, Iglesias A, Bertran J, Alvarez D, Garcia-Prieto CA, Ballare C, Rodriguez-Cortez V, et al.supplemented with 20 ng/ml activin A (Cat. #120-14E; PeproTech) and 10 ng/ml Fgf basic (R&D Systems, Cat #233-FB-025/CF). Cells were submitted to daily media changes till day 5 (120 hr), when they were further analyzed. Embryoid bodies

(7) Proteomic landscape of porcine induced neural stem cell reprogramming and differentiationPeerJOctober 3, 2025Sekkarin Ploypetch, Sataporn Phochantachinda, Warunya Chakritbudsabong, Walasinee Sakcamduang, Nattarun Chaisilp, Somjit Chaiwattanarungruengpaisan, Supitcha Pannengpetch, Piyada Na Nakorn, Tharathip Muangthong, Sasitorn Rungarunlertand 20 ng/mL human basic fibroblast growth factor (bFGF; R&D Systems, Minneapolis, MN, USA; cat. no. 233-FB-025/CF). Cells were seeded on Matrigel-coated 6 cm tissue culture dishes at a density of 1.5 × 104 cells/cm2

(8) Low concentration press cake protein isolates preserve biological activity during lyophilization and spray dryingFrontiers in NutritionJune 18, 2025Kristina Egger, Lisa Schenzle, Afonso Urich Andreina Isabel, Manuel Zettl, Aleksandra Fuchs, Harald Pichlerfetal bovine serum (FBS; ThermoFisher #26140079) and 1 ng/mL human FGF basic/FGF2/bFGF (R&D Systems #233-FB-025/CF). After passage one, P-GM was changed to growth medium (GM) where Primocin is replaced by 1% Antibiotic-Antimycotic

(9) Low-cost food-grade alternatives for serum albumins in FBS-free cell culture mediaScientific reportsMay 1, 2025Schenzle L, Egger K, Spangl B, Hussein M, Ebrahimian A, Kuehnel H, Ferreira FC, Marques DMC, Berchtold B, Borth N, et al.fetal bovine serum (FBS; ThermoFisher #26140079) and 1 ng/mL human FGF basic/FGF2/bFGF (R&D Systems #233-FB-025/CF). After passage one, P-GM was changed to growth medium (GM) where Primocin is replaced by 1% Antibiotic-Antimycotic

(10) AKT1 as a therapeutic target for platinum-resistant SOX2 positive ovarian cancer cellsSci RepApril 29, 2025Mengyang Xue, Li Kang, Yunfeng Zhang, Xixia Yuan, Jiwen Li, Rong Zhang, Jiemin Wong236-EG01M), and 10 ng/ml recombinant human basic fibroblast growth factor (bFGF; R&D Systems Cat. no. 233-FB-025/CF), 1% B27 supplement (Thermo Fisher Scientific Cat. no.17504044), 1% insulin (Thermo Fisher Scientific

(11) Neural regulation of H3K27me3 during the induction of patterning competency in regenerating Axolotl limb cellsCommun BiolApril 24, 2025Michael J. Raymond, Matthew A. Cherubino, Warren A. Vieira, Sheyla Manon, Catherine D. McCuskerFGF8, or 300 ng/μL BMP2 + FGF8 of each growth factor (BMP2:355-BM-050/CF, FGF8a:4745-F8-050, FGFbasic:233-FB-025/CF, R&D Systems) and implanted into the wound site as previously described20. For growth factor inhibitor

(12) Mitochondrial fusion and cristae reorganization facilitate acquisition of cardiomyocyte identity during reprogramming of murine fibroblastsCell ReportsMarch 25, 2025Brian M. Spurlock, Yifang Xie, Yiran Song, Shea N. Ricketts, James Rock Hua, Haley R. Chi, Meenakshi Nishtala, Rustem Salmenov, Jiandong Liu, Li QianBuffer,Thermofisher,Cat# 89900 Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein,R&D Systems,Cat# 233-FB-025/CF Recombinant Human FGF-10 Protein,R&D Systems,Cat# 345-FG-025/CF Recombinant Human VEGF 165 Protein

(13) Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteriesEuropean Journal of Cell BiologyMarch 1, 2025Javier Abello, Ying Yin, Yonghui Zhao, Josh Maurer, Jihui Lee, Cherokee Bodell, Jahmiera Richee, Abigail J. Clevenger, Zarek Burton, Megan E. Goeckel, et al.hydrogel contains 2.5 mg/ml Collagen I from rat tail (Corning, Cat.#: 354249), FGF (R&D systems, Cat.#: 233-FB-025/CF), SCF (R&D systems, Cat.#: 255-SC-010/CF), IL3 (R&D systems, Cat.#: 203-IL-010/CF), SDF1a (Cat.#: 350-NS-010/CF)

(14) Targeting AKT as a promising strategy for SOX2-positive, chemoresistant osteosarcomaBone ResFebruary 24, 2025Yujie Liu, Li Kang, Jing Luo, Minglei Yang, Da Wang, Juelan Ye, Xinghai Yang, Wei Wan, Jiemin Wong, Jianru Xiao236-EG-01M), and 10 ng/mL human recombinant basic fibroblast growth factor (bFGF) (R&D Systems, Cat. No. 233-FB-025/CF) in a humidified 5% CO2 incubator at 37 °C. The serum-free medium was changed every other day. Sphere

(15) Small extracellular vesicles derived from umbilical cord mesenchymal stem cells alleviate radiation-induced cardiac organoid injuryStem Cell Research & TherapyDecember 20, 2024Hu Cao, Liang Yue, Jingyuan Shao, Fanxuan Kong, Shenghua Liu, Hongyu Huai, Zhichao He, Zhuang Mao, Yuefeng Yang, Yingxia Tan, et al.attachment 96-well plates (Corning) in CDM medium [24] supplemented with bFGF (30 ng/mL, R&D Systems, 233-FB-025/CF), LY294002 (5 µM, Tocris, 1130), activin A (50 ng/mL, Sigma‒Aldrich, SRP3003), BMP4 (10 ng/mL, R&D Systems,

(16) Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytesNature communicationsNovember 27, 2024Lim AA, Pouyabahar D, Ashraf M, Huang K, Lohbihler M, Murareanu BM, Chang ML, Kwan M, Alibhai FJ, Tran T, et al.

(17) Dissecting TGF-β-induced glioblastoma invasion with engineered hyaluronic acid hydrogelsAPL BioengineeringJune 13, 2024Kwasi Yeboa Amofa, Katherine Michelle Patterson, Jessica Ortiz, Sanjay Kumarsupplement (Gibco, 17504-044), 20 ng/ml EGF (R&D Systems, 236-EG-01M), and 20 ng/ml FGF (R&D Systems, 233-FB-025/CF). For 3D HA gel experiments, the medium was supplemented with 0.1% penicillin/streptomycin (Thermo Fisher Scientific

(18) Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1bioRxiv : the preprint server for biologyApril 1, 2024Johnson D, Colijn S, Richee J, Yano J, Burns M, Davis AE, Pham VN, Saric A, Jain A, Yin Y, et al.

(19) Evaluation of Zonulin Expression and Its Potential Clinical Significance in GlioblastomaCancers (Basel)January 14, 2024Roberta Repossi, Rita Martín-Ramírez, Fuensanta Gómez-Bernal, Lilian Medina, Helga Fariña-Jerónimo, Rebeca González-Fernández, Pablo Martín-Vasallo, Julio Plata-BelloR&D systems, McKinley Place NE, MN, USA) 20 ng/mL, basic fibroblast growth factor (bFGF, Catalog No. 233-FB-025/CF; R&D systems, McKinley Place NE, MN, USA) 20 ng/mL, and B-27 additive 1X (Catalog No. A3353501; Gibco,

(20) Pharmacological inhibition of MYC to mitigate chemoresistance in preclinical models of squamous cell carcinomaTheranosticsJanuary 1, 2024Liu S, Qin Z, Mao Y, Wang N, Zhang W, Wang Y, Chen Y, Jia L, Peng X.236-EG-01M), and human recombinant basic fibroblast growth factor (bFGF; 10 ng/mL; R&D Systems, Cat# 233-FB-025/CF), in an incubator at 37 °C with a 5% CO2 atmosphere. Extreme Limiting-dilution assay and the orthotopic

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FGF basic/FGF2/bFGF (146 aa) Protein

FGF basic/FGF2/bFGF (146 aa) Protein

FGF basic/FGF2/bFGF (146 aa) Protein

FGF basic/FGF2/bFGF (146 aa) Protein

Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein, CF from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein, CF from R&D Systems, a Bio-Techne Brand

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