Fig 1: The effect of IL‐7 and sCD127 on CD127 expression on CD4+ and CD8+ T‐cells in healthy C57BL/6 mice. (A) Experimental timeline of healthy age‐matched female C57BL/6 mice. Mice were divided into four groups, and treated for 5 consecutive days either with sCD127 alone, IL‐7 alone, a combined dose of sCD127 and IL‐7 (preincubated at 37°C for 30 min), or PBS. (B) Flow cytometric analysis of CD127 expression on CD4+ and CD8+ T‐cells. (C) The proportion of CD4+ T‐cells expressing CD127 (C, left), and CD8+ T‐cells expressing CD127 (C, right) in the blood before treatment (Day 3), 6 h posttreatment (6 Hrs), and 24 h posttreatment (24 Hrs). (D) CD127 MFI in CD4+ T‐cells (D, left) and CD8+ T‐cells (D, right) normalized to CD127 MFI of a PBS‐treated group. Data are pooled from two independent experiments, n = 8 mice per group. Data represent mean ± SD and statistical analysis was completed using a one‐way ANOVA for more than two groups, and an unpaired nonparametric Mann–Whitney test was used for comparisons between the two groups. p Values are listed as either not significant (ns, p > .05) or significant, with p values grouped as follows: *p < .05, **p < .01, ***p < .001, or ****p < .0001. An asterisk above a column bar represents a statistically significant difference between the indicated treatment and PBS‐treated mice at the same time point. ANOVA, analysis of variance; PBS, phosphate‐buffered saline