Fig 1: Secreted proteins regulated by food-deprivation.(A, B) Secreted protein genes differentially expressed between AGRP and POMC neurons (A) or AGRP or POMC neurons FD/fed (B). Genes mentioned in the text are labeled with an asterisk. (C) Double smFISH for Agrp and Ccl17. Scale, 10 μm. (D, E) Population counts (bars: mean values) (D) and cumulative probability distributions (E) of Ccl17 puncta per cell volume in AGRP neurons (p = 1.5e−20, ks-test). Fed, n = 144; FD, n = 230; 3 mice per condition. (F) Food intake at start of light period 1-hr and 2-hr after intracerebroventricular injection of either saline or recombinant CCL17 (500 ng). Rank-sum test. (G) Cre-dependent viral vector for cell type-specific Ccl17 overexpression in AGRP neurons. hSyn: synapsin promoter. Black and white triangles denote heterotypic loxP sites for stable inversion of Ccl17-IRES-GFP. (H) Schematic for viral transduction and cell-type specific overexpression of Ccl17 in the brains of AgrpCre mice. (I) Representative image showing Ccl17-IRES-GFP-transduced AGRP neurons. Scale, 100 µm. (J) Body weight change from pre-injection weight (starting age: 8 weeks) in AgrpCre mice expressing Ccl17-IRES-GFP or BFP (2-way ANOVA, one factor repeated measures, transgene: F1,65 = 12.0, p = 0.004; time: F5,65 = 14.8, p < 0.001; interaction: F5,65 = 14.8, p < 0.001). BFP data is same as Figure 5I. Holm-Sidak correction for multiple comparisons. AGRPCcl17 n = 9 mice, AGRPBFP n = 7 mice. Data is mean ± s.e.m. n.s., p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.DOI: http://dx.doi.org/10.7554/eLife.09800.014