Fig 1: The inflammatory markers IL-18, CXCL1, and OSM do not modulate the NKCC1 activity. a Schematic illustration of the inflammatory markers in cocktail 2 (IL-18, CXCL1, and OSM) and their corresponding receptors. b 86Rb+ efflux from the rat choroid plexus as a function of time in the control setting (black, n = 3) or with exposure to cocktail 1 (blue, n = 4) containing IL-18 (200 ng/ml), CXCL1 (100 ng/ml), and OSM (200 ng/ml). The y-axis represents the natural logarithm of the 86Rb+ amount left in the choroid plexus at time T (AT) divided by the initial amount at time 0 (A0). c 86Rb+ efflux rate constant in the control setting (0.79 ± 0.10 min−1, n = 3) or with exposure to cocktail 2 (0.80 ± 0.16 min−1, n = 4) containing IL-18 (200 ng/ml), CXCL1 (100 ng/ml), and OSM (200 ng/ml). P = 0.94. d 86Rb+ efflux rate constant in the control setting (0.90 ± 0.03 min−1, n = 3) or with exposure to high concentrations of cocktail 2 (0.94 ± 0.07 min−1, n = 4, IL-18 (500 ng/ml), CXCL1 (500 ng/ml), and OSM (500 ng/ml)), P = 0.32. Error bars in b–d represent standard deviation and statistical significance was tested with an unpaired two-tailed t-test. NS, not significant