IFN-gamma Protein 485-MI-100 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIFN-gamma Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $503.00Supplier Page View Company Product Page
Catalog Number485-MI-100
SpeciesMouse
Molecule NameIFN-gamma
Size100 ug
Source/Expression SystemE. coli-derived Recombinant Mouse IFN-gamma Protein
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFN-g, If2f, IMD69, Ifg, IFG, IFI
FormatLyophilized from a 0.2 µm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP with BSA as a carrier protein
Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
ApplicationsBioactivity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 100 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1Redox BiologyJune 1, 2026Nicholas L. Arp, Uzziah S. Urquiza, Marcel Morgenstern, Jonathan H. Schrope, James A. Votava, Steven V. John, Jack J. Stevens, Felicia Deng, Anna Huttenlocher, Joshua J. Coon, et al.Escherichia coli O111:B4 (Sigma-Aldrich, L3024) and 30 ng/mL recombinant mouse interferon-γ (IFNγ; R&D Systems, 485-MI-100) for the indicated durations; RAW264.7 cells were treated with 50 ng/mL LPS and 10 ng/mL IFNγ for the

(2) Organ injury in systemic autoimmunity is mediated by stem-like CD8+ T cells arising from tissue-draining lymph nodesImmunityApril 20, 2026Jafar Al Souz, Yulong Wei, Can Cui, Rihao Qu, Jin-Young Choi, Fang Wang, Steven J. Moioffer, Julia Barrett, Irene Chernova, Manvitha Nadella, et al.Cell Stain Kit,Invitrogen,Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier,R&D Systems,Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80–235) Protein with carrier,R&D Systems,Cat#410-MT-025 Recombinant Mouse IL-10 Protein

(3) Discovery of metabolites produced by reactions between central carbon metabolites and cysteine that mark inflammatory macrophagesbioRxivMarch 23, 2026Nicholas L. Arp, Felicia Deng, Jorgo Lika, Gretchen L. Seim, Paulo Falco Cobra, Carlos Mellado Fritz, Steven V. John, Swetha Rathinaraj, Bridget E. Shields, Daniel Amador-Noguez, et al.ng/mL LPS (E. coli O111:B4; Sigma-Aldrich, L3024) and 30 ng/mL recombinant mouse IFNγ (R&D Systems, 485-MI-100) for the indicated durations. For alternative activation, BMDMs were treated with with 20 ng ml−1 IL-4

(4) Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in miceNature MicrobiologyMarch 19, 2026Li Yang, Robert C. Peery, Shirui Zhou, Xiaomin Chen, Leah M. Farmer, Fabiola Gutierrez, Stephanie Fowler, Lanjing Zhang, Julia M. Salamat, Karen Riggins, et al.days. For IFNγ treatment, GF-derived organoids were incubated with recombinant mouse IFNγ (R&D Systems, 485-MI-100) at 0.1 ng ml−1 for 0, 24, 48, 72 or 96 h before collection. For single-agent treatment, organoids were

(5) MAPK Pathway Mutations Emerge in Mutant-IDH1 Inhibitor-Resistant Cholangiocarcinoma and Attenuate the Interferon ResponseClinical Cancer ResearchMarch 2, 2026Jinkai Wan, Hatice Duygu Saatcioglu, Haley Ellis, Elia Aguado-Fraile, Qin Xu, Hiroshi Kondo, Robert T. Manguso, Ilaria Gritti, Bryanna L. Norden, Carolina Noble, et al.(Recombinant Human IFN-gamma Protein, R&D Systems, cat. #285-IF-100; or Recombinant Mouse IFN-gamma Protein, R&D Systems, cat. #485-MI-100). Note: RAS inhibitors were added at least 6 hours before IFNγ treatment to pre-reduce MAPK signaling. Immunoblotting Cell

(6) Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitisbioRxivFebruary 7, 2026Charlotte R. Wayne, Ugur Akcan, Travis E. Faust, Violetta Duran-Laforet, Danny Jamoul, Luca Bremner, Nicole Ampatey, Büşra T. Akcan, Sarah J. Ho, Bogoljub Ciric, et al.switched to 2% FBS without growth factor supplements. Media containing either mouse IFNγ (R&D Systems, 485-MI-100), mouse IL-17A (R&D Systems, 7956-ML-025), mouse GM-CSF (R&D Systems, 415-ML-010) or vehicle (PBS with

(7) Lymphotoxin-driven cancer cell eradication by tumoricidal CD8 + TILbioRxivJanuary 16, 2026Hongyan Xie, Aiping Jiang, Aonkon Dey, Joseph W. Dean, Jonathan J. Perera, Neal P. Smith, Alex C. Y. Chen, Seth Anderson, Angelina M. Cicerchia, Yi Sun, et al.#285-IF-100), human TNFα (160 ng/mL, R&D Systems, #10291-TA-100), mouse IFNγ (40 ng/mL, R&D Systems, #485-MI-100). For inhibitor studies, cells were pretreated for 2 hours with the indicated doses of ruxolitinib (MedChemExpress,

(8) Islet-intrinsic sex differences in inflammatory signaling contribute to autoimmune diabetes susceptibilitybioRxivJanuary 16, 2026Kierstin L. Webster, Armando A. Puente, Jacob R. Enriquez, Soumyadeep Sarkar, Ernesto S. Nakayasu, Bobbie-Jo M. Webb-Robertson, Lisa M. Bramer, Kayla Figatner, Sarida Pratuangtham, Wenting Wu, et al.401-ML-010), 50 ng/mL mouse TNF-α (R&D Systems; 410-MT-010), and 100 ng/mL mouse IFN-γ (R&D Systems; 485-MI-100) for 18 hours, or with 17-β estradiol (E2) (Millipore Sigma, E2758) (concentration and duration detailed

(9) MAPK Pathway Mutations Emerge in Mutant IDH1 Inhibitor–Resistant Cholangiocarcinoma and Attenuate the IFN ResponseClin Cancer ResDecember 29, 2025Jinkai Wan, Hatice Duygu Saatcioglu, Haley Ellis, Elia Aguado-Fraile, Qin Xu, Hiroshi Kondo, Robert T. Manguso, Ilaria Gritti, Bryanna L. Norden, Carolina Noble, et al.(Recombinant Human IFN-gamma Protein, R&D Systems, cat. #285-IF-100; or Recombinant Mouse IFN-gamma Protein, R&D Systems, cat. #485-MI-100). Note: RAS inhibitors were added at least 6 hours before IFNγ treatment to pre-reduce MAPK signaling. Immunoblotting Cell

(10) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia Lei100 ng/mL LPS (00-4976-93, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and 20 ng/mL IFNγ (485-MI-100, R&D Systems, Minneapolis, MN, USA) for M1, and 20 ng/mL IL-4 (404-ML-010, R&D Systems, Minneapolis,

(11) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia Lei100 ng/mL LPS (00-4976-93, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and 20 ng/mL IFNγ (485-MI-100, R&D Systems, Minneapolis, MN, USA) for M1, and 20 ng/mL IL-4 (404-ML-010, R&D Systems, Minneapolis,

(12) Decoding adult murine pancreatic islet cell diversity through cell type-resolved proteomics and phosphoproteomicsCommun BiolOctober 17, 2025Marvin Thielert, Adrian Villalba, Vincenth Brennsteiner, Maria Wahle, Constantin Ammar, Andreas-David Brunner, Alexis Fouque, Chloé Lourenço, Masaya Oshima, Latif Rachdi, et al.(#15140122, ThermoFisher Scientific). Next, islets were treated with or without IFNγ (5 ng/ml) for 20 h (#485-MI-100, R&D Systems, Minneapolis, MN, USA). For IFNγ treatment, pooled islets from 3 mice per condition were

(13) Leukocyte-type 12/15-lipoxygenase is essential for timely inflammation-resolution and effective tissue regeneration following skeletal muscle injuryMolecular metabolismOctober 1, 2025Sharma B, Lu X, Rehman H, Figueiredo VC, Davis C, Van Remmen H, Kuang S, Brooks SV, Maddipati KR, Markworth JF.

(14) Neutrophil Extracellular Traps Modulate Recruitment and Immunosuppression of Macrophages in Pancreatic AdenocarcinomaCancer Immunol ResOctober 1, 2025Hillary G. Pratt, Alyson M. Stevens, Michael Sestito, Mercy Ojetunde, Abby D. Ivey, Nicole E. Mihalik, Kayla J. Steinberger, Britney Niemann, E. Hannah Hoblitzell, Edwin Wan, et al.24-well plate at 30,000 cells per well overnight. The cells were then treated with IFNγ (Bio-Techne, 485-MI-100) diluted in the corresponding serum-free medium. Media were collected after 24 hours and centrifuged

(15) Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophilseLifeSeptember 12, 2025Ravesloot-Chavez MM, Van Dis E, Fox D, Anaya-Sanchez A, Espich S, Nguyenla XH, Rawal S, Samani H, Ballinger M, Thomas HF, et al.

(16) Vector-free intra-airway in vivo epigenetic editingTrends in biotechnologySeptember 1, 2025Yano N, Ramar M, Gregory DJ, Fedulov AV.

(17) Macrophage–Kruppel-Like Transcription Factor 6 Signaling Promotes Experimental AtherogenesisThe American Journal of PathologySeptember 1, 2025Hang Pong Ng, Atif Zafar, Rachel Diamond-Zaluski, Gun-Dong Kim, Kartik Bhat, Owen Meadows, Yashwant Pantra, E. Ricky Chan, Jonathan D. Smith, Ganapati H. Mahabeleshwarfactor (416-ML), mouse tumor necrosis factor (TNF)-α protein (210-TA-100), and mouse interferon-γ (485-MI-100) were obtained from R&D Systems (Minneapolis, MN). The high-glucose Dulbecco's modified Eagle's medium

(18) Evidence that mitochondria in macrophages are destroyed by microautophagyNature communicationsAugust 30, 2025Lu SL, Chen S, Noda K, Li Y, Tsai CY, Omori H, Kato Y, Zhang Z, Chen B, Tokuda K, et al.

(19) Endolysosomal damage surveillance enables rapid inflammasome sensing of pathogensCell reportsAugust 26, 2025Shivcharan S, Younis DA, Wright SS, Wang C, Behl B, Shumba P, Delgado KN, Chawla AK, Alto NM, Panjwani N, et al.16008 Carbenicillin antibiotic,Boston Bioproducts,Cat# P-760 Recombinant mouse IFN-γ,R&D Systems,Cat# 485-MI-100 Gentamicin,Gibco,Cat#15750-060 Streptomycin sulfate,Sigma-Aldrich,Cat# S6501-100G Clarity ECL HRP

(20) Inflammatory cytokine interleukin-18 prevents murine miscarriage by inducing appropriate inflammationScientific reportsAugust 21, 2025Horii Y, Ino H, Sasaki F, Ishibashi M, Koike E, Kawamoto Y, Flavell RA, Ohashi R, Suzuki S, Negishi Y, et al.

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Recombinant Mouse IFN-gamma Protein

IFN-gamma Protein

IFN-gamma Protein

IFN-gamma Protein

IFN-gamma Protein from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (542)

Reviews

IFN-gamma Protein from R&D Systems, a Bio-Techne Brand

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