Fig 1: Elevated P-selectin levels in peripheral blood plasma and retina in EIOP-induced experimental glaucoma mice. (A) Elevated plasma sP-selectin levels in the glaucoma mice (n = 10) compared with controls (Ctrls) (n = 6). (B, C) Retinal transcriptomic analysis of the publicly available GEO dataset GSE297955 showed increased expression of the genes encoding Selp and Selplg. (D, E) Retinal Selp and Selplg mRNA expression levels in glaucomatous mice 20 days after EIOP induction compared with Ctrl mice (Selp: glaucoma, n = 6; Ctrl, n = 11; Selplg: glaucoma, n = 6; control, n = 8). n = number of RNA test; one retina sample has two retinas, which are from two distinct mice. All the results represent one of the three independent experiments. Statistical analyses were conducted using (A) Welch's t-test, (D, E) the Mann–Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001. Boxplots represent the median and IQR. Gene symbols: Selp, P-selectin; Selplg, P-selectin glycoprotein ligand 1.